MiRNA-181b suppresses IGF-1R and functions as a tumor suppressor gene in gliomas
- Zhu-mei Shi1,7,
- Xie-feng Wang1,7,
- Xu Qian2,7,
- Tao Tao1,
- Lin Wang2,
- Qiu-dan Chen2,
- Xi-rui Wang3,
- Lei Cao4,
- Ying-yi Wang1,
- Jun-xia Zhang1,
- Tao Jiang5,
- Chun-sheng Kang6,
- Bing-hua Jiang2,
- Ning Liu1,8 and
- Yong-ping You1,8
- 1Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China
- 2State Key Lab of Reproductive Medicine, Department of Pathology and Cancer Center, Nanjing Medical University, Nanjing 210029, China
- 3Department of Neurosurgery, Cangzhou Central Hospital, Cangzhou 061001, Hebei Province, China
- 4Department of Neurosurgery, Xuzhou Central Hospital, Affiliated Hospital of Medical College of Southeast University, Xuzhou 221009, Jiangsu, China
- 5Department of Neurosurgery, Tiantan Hospital, Capital Medical University, Beijing 100050, China
- 6Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, China
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↵7 These authors contributed equally to this work.
Abstract
MicroRNAs (miRNAs) are single-stranded, 18- to 23-nt RNA molecules that function as regulators of gene expression. Previous studies have shown that microRNAs play important roles in human cancers, including gliomas. Here, we found that expression levels of miR-181b were decreased in gliomas, and we identified IGF-1R as a novel direct target of miR-181b. MiR-181b overexpression inhibited cell proliferation, migration, invasion, and tumorigenesis by targeting IGF-1R and its downstream signaling pathways, PI3K/AKT and MAPK/ERK1/2. Overexpression of IGF-1R rescued the inhibitory effects of miR-181b. In clinical specimens, IGF-1R was overexpressed, and its protein levels were inversely correlated with miR-181b expression. Taken together, our results indicate that miR-181b functions in gliomas to suppress growth by targeting the IGF-1R oncogene and that miR-181b may serve as a novel therapeutic target for gliomas.
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Footnotes
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↵8 Corresponding authors
E-mail YYPL9{at}njmu.edu.cn
E-mail liuning0853{at}126.com
- Received August 13, 2012.
- Accepted January 14, 2013.
- Copyright © 2013 RNA Society
