Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing

GUNTER MEISTER; MARKUS LANDTHALER; YAIR DORSETT; THOMAS TUSCHL

doi:10.1261/rna.5235104

Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing

Laboratory of RNA Molecular Biology, The Rockefeller University, New York, New York 10021, USA

Abstract

A large number of miRNAs have recently been discovered in plants and animals. Development of reverse genetic approaches that act to inhibit microRNA function would facilitate the study of this new class of noncoding RNA. Here we show that 2′-O-methyl oligoribonucleotides, but not 2′-deoxyoligonucleotides specifically inactivate the RNAi activity associated with miRNA–protein complexes in human cell extracts as well as in cultured human cells.

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Footnotes

↵1 These authors contributed equally to this work.
Article and publication are at http://www.rnajournal.org/cgi/doi/10.1261/rna.5235104.
- Accepted December 11, 2003.
- Received November 18, 2003.