Gene Expression in Blastocysts Following Direct Injection of DNA Into Testis

Shyoso OGAWA; Katsuhiko HAYASHI; Norihiro TADA; Masahiro SATO; Takashi KURIHARA; Makoto IWAYA

doi:10.1262/jrd.41.379

Research Notes

Gene Expression in Blastocysts Following Direct Injection of DNA Into Testis

Shyoso OGAWA, Katsuhiko HAYASHI, Norihiro TADA, Masahiro SATO, Takashi KURIHARA, Makoto IWAYA

Author information

Keywords: Testis-mediated gene transfer, Transgenic embryo, Expression of exogenous DNA, β-Galactosidase gene, Neomycin resistance gene, PCR, Mice

JOURNAL FREE ACCESS

1995 Volume 41 Issue 4 Pages 379-382

DOI https://doi.org/10.1262/jrd.41.379

Details

Abstract

Liposome-encapsulated plasmid pMT-neo/MT-β-gal DNA containing a neomycin resistance gene and a E. coli β-galactosidase gene was injected through a needle into mature mouse testes bilaterally 3 times at 4-day intervals. Eighty percent of blastocysts derived from eggs fertilized by spermatozoa of male mice injected with the DNA exhibited expression of β-galactosidase gene and the presence of pMT-neo/MT-β-gal DNA. Thus, direct injection of foreign DNA into the testis will provide a novel and convenient tool for production of transgenic animals.

Corresponding author

Register with J-STAGE for free!