Construction of BAC libraries derived from the ascidian Ciona intestinalis

Mari Kobayashi; Masaru Matsuda; Shuichi Asakawa; Nobuyoshi Shimizu; Yoshitaka Nagahama; Yutaka Satou; Nori Satoh

doi:10.1266/ggs.77.283

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Construction of BAC libraries derived from the ascidian Ciona intestinalis

Mari Kobayashi, Masaru Matsuda, Shuichi Asakawa, Nobuyoshi Shimizu, Yoshitaka Nagahama, Yutaka Satou, Nori Satoh

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JOURNAL FREE ACCESS

2002 Volume 77 Issue 4 Pages 283-285

DOI https://doi.org/10.1266/ggs.77.283

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Abstract

Large insert genomic bacterial artificial chromosome (BAC) libraries were constructed from a basal chordate, the ascidian Ciona intestinalis. Insert analyses of randomly selected clones indicated that in the first library the mean insert size was 135 kb and predicted a 15-fold coverage of the Ciona genome, and in the second library the mean insert size was 165 kb and predicted a 5-fold coverage of the genome. These first large insert genomic libraries of the ascidian should increase the speed of genomic analyses of basal chordates.

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