Mapping quantitative trait loci for heat tolerance at anthesis in rice using chromosomal segment substitution lines

Lei Zhao; Jianguo Lei; Yingjin Huang; Shan Zhu; Hongping Chen; Renliang Huang; Zhiqin Peng; Qinghua Tu; Xianhua Shen; Song Yan

doi:10.1270/jsbbs.15084

Research Papers

Mapping quantitative trait loci for heat tolerance at anthesis in rice using chromosomal segment substitution lines

Lei Zhao, Jianguo Lei, Yingjin Huang, Shan Zhu, Hongping Chen, Renliang Huang, Zhiqin Peng, Qinghua Tu, Xianhua Shen, Song Yan

Author information

Lei Zhao
Rice National Engineering Laboratory (Nanchang), Jiangxi Provincial Key Laboratory of Rice Physiology and Genetics, Rice Research Institute, Jiangxi Academy of Agricultural Sciences
College of Agronomy, Jiangxi Agricultural University
Key Laboratory of Agriculture Responding to Climate Change
Jianguo Lei
Rice National Engineering Laboratory (Nanchang), Jiangxi Provincial Key Laboratory of Rice Physiology and Genetics, Rice Research Institute, Jiangxi Academy of Agricultural Sciences
College of Agronomy, Jiangxi Agricultural University
Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education
Yingjin Huang
College of Agronomy, Jiangxi Agricultural University
Key Laboratory of Agriculture Responding to Climate Change
Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education
Shan Zhu
Rice National Engineering Laboratory (Nanchang), Jiangxi Provincial Key Laboratory of Rice Physiology and Genetics, Rice Research Institute, Jiangxi Academy of Agricultural Sciences
College of Agronomy, Jiangxi Agricultural University
Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education
Hongping Chen
Rice National Engineering Laboratory (Nanchang), Jiangxi Provincial Key Laboratory of Rice Physiology and Genetics, Rice Research Institute, Jiangxi Academy of Agricultural Sciences
Renliang Huang
Rice National Engineering Laboratory (Nanchang), Jiangxi Provincial Key Laboratory of Rice Physiology and Genetics, Rice Research Institute, Jiangxi Academy of Agricultural Sciences
Zhiqin Peng
Rice National Engineering Laboratory (Nanchang), Jiangxi Provincial Key Laboratory of Rice Physiology and Genetics, Rice Research Institute, Jiangxi Academy of Agricultural Sciences
College of Agronomy, Jiangxi Agricultural University
Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education
Qinghua Tu
Rice National Engineering Laboratory (Nanchang), Jiangxi Provincial Key Laboratory of Rice Physiology and Genetics, Rice Research Institute, Jiangxi Academy of Agricultural Sciences
Jiangxi Seed Administration
Xianhua Shen
Rice National Engineering Laboratory (Nanchang), Jiangxi Provincial Key Laboratory of Rice Physiology and Genetics, Rice Research Institute, Jiangxi Academy of Agricultural Sciences
Song Yan
Rice National Engineering Laboratory (Nanchang), Jiangxi Provincial Key Laboratory of Rice Physiology and Genetics, Rice Research Institute, Jiangxi Academy of Agricultural Sciences

Keywords: rice (Oryza sativa L.), heat tolerance, anthesis stage, CSSLs, QTL, qPSL^ht4.1

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Supplementary material

2016 Volume 66 Issue 3 Pages 358-366

DOI https://doi.org/10.1270/jsbbs.15084

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Abstract

To study the genetic basis of heat tolerance at anthesis, a set of chromosome segment substitution lines (CSSLs) derived from Sasanishiki (japonica ssp. heat susceptible) and Habataki (indica spp. heat tolerant) were used for analysis across three high temperature environments. Spikelet fertility (SF), daily flowering time (DFT) and pollen shedding level (PSL) under high temperature (HT) were assessed. Eleven related QTLs were detected, of which, two QTLs qSF^ht2 and qSF^ht4.2 for spikelet fertility were identified on chromosomes 2 and 4. Four QTLs qDFT3, qDFT8, qDFT10.1 and qDFT11 for daily flowering time were detected on chromosomes 3, 8, 10 and 11. The other five QTLs qPSL^ht1, qPSL^ht4.1, qPSL^ht5, qPSL^ht7 and qPSL^ht10.2 on chromosomes 1, 4, 5, 7 and 10, respectively, were found had effects both on spikelet fertility and pollen shedding level. Of the 11 QTLs, 8 were overlapped with QTLs reported by others, 3 QTLs qPSL^ht4.1, qPSL^ht7 and qPSL^ht10.2 identified in this study were novel. The stability of qPSL^ht4.1 was further verified at different temperatures, which could be used to improve the pollen shedding and pollen growth on stigma for rice heat-tolerance breeding.

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