Mapping of Disease-Related Genes in Japanese Pear using a Molecular Linkage Map with RAPD Markers

Hiroyuki Iketani; Kazuyuki Abe; Toshiya Yamamoto; Kazuo Kotobuki; Yoshihiko Sato; Toshihiro Saito; Osamu Terai; Nagao Matsuta; Tateki Hayashi

doi:10.1270/jsbbs.51.179

Research papers

Mapping of Disease-Related Genes in Japanese Pear using a Molecular Linkage Map with RAPD Markers

Hiroyuki Iketani, Kazuyuki Abe, Toshiya Yamamoto, Kazuo Kotobuki, Yoshihiko Sato, Toshihiro Saito, Osamu Terai, Nagao Matsuta, Tateki Hayashi

Author information

Hiroyuki Iketani
National Institute of Fruit Tree Science
Present address: National Agricultural Research Organization
Kazuyuki Abe
National Institute of Fruit Tree Science
Present address: Yamagata Horticultural Experiment Station
Toshiya Yamamoto
National Institute of Fruit Tree Science
Kazuo Kotobuki
National Institute of Fruit Tree Science
Yoshihiko Sato
National Institute of Fruit Tree Science
Toshihiro Saito
National Institute of Fruit Tree Science
Osamu Terai
National Institute of Fruit Tree Science
Present address: Nagasaki Prefectural Fruit Tree Experiment Station
Nagao Matsuta
National Institute of Fruit Tree Science
Tateki Hayashi
National Institute of Fruit Tree Science

Keywords: linkage map, Japanese pear, RAPD, pear scab, black spot, disease-related gene

JOURNAL FREE ACCESS

2001 Volume 51 Issue 3 Pages 179-184

DOI https://doi.org/10.1270/jsbbs.51.179

Details

Abstract

Molecular linkage maps of disease-related genes in the Japanese pear cultivars ‘Kinchaku’ and ‘Kosui’ with RAPD markers were constructed. Using the pseudotestcross method, two separate maps were constructed from the segregation data of 82 F₁ individuals. The linkage map for ‘Kinchaku’ consisted of 120 loci in 18 linkage groups spanning 768cM, while that for ‘Kosui’ contained 78 loci in 22 linkage groups extending over 508cM. The resistance allele of pear scab and the susceptibility allele of black spot were mapped in different linkage groups in ‘Kinchaku’. Compared with the reports on apple genome mapping, we estimated that these maps covered at least about a half of the total pear genome.

Corresponding author

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