Identification of Quantitative Trait Loci Controlling Cold Tolerance at the Reproductive Stage in Yunnan Landrace of Rice, Kunmingxiaobaigu

Luyuan Dai; Xinghua Lin; Changrong Ye; Kazuo Ise; Koji Saito; Akira Kato; Furong Xu; Tengqiong Yu; Duanpin Zhang

doi:10.1270/jsbbs.54.253

Research paper

Identification of Quantitative Trait Loci Controlling Cold Tolerance at the Reproductive Stage in Yunnan Landrace of Rice, Kunmingxiaobaigu

Luyuan Dai, Xinghua Lin, Changrong Ye, Kazuo Ise, Koji Saito, Akira Kato, Furong Xu, Tengqiong Yu, Duanpin Zhang

Author information

Luyuan Dai
Crop Genetic Resources Institute, Yunnan Academy of Agricultural Sciences
Huazhong Agricultural University
Xinghua Lin
Huazhong Agricultural University
Changrong Ye
Crop Genetic Resources Institute, Yunnan Academy of Agricultural Sciences
Kazuo Ise
Japan International Research Center for Agricultural Sciences
Koji Saito
National Agricultural Research Center for Hokkaido Region
Akira Kato
National Agricultural Research Center for Hokkaido Region
Furong Xu
Crop Genetic Resources Institute, Yunnan Academy of Agricultural Sciences
Tengqiong Yu
Crop Genetic Resources Institute, Yunnan Academy of Agricultural Sciences
Duanpin Zhang
Huazhong Agricultural University

Keywords: Oryza sativa L., cold tolerance, Kunmingxiaobaigu, quantitative trait loci (QTLs), reproductive stage

JOURNAL FREE ACCESS

2004 Volume 54 Issue 3 Pages 253-258

DOI https://doi.org/10.1270/jsbbs.54.253

Details

Abstract

This study focused on a Yunnan landrace of rice, Kunmingxiaobaigu, to identify quantitative trait loci (QTLs) controlling cold tolerance at the reproductive stage using DNA markers. A linkage map with 16 linkage groups covering 1,354.4 cM with a total of 122 markers was constructed based on an F₂ population consisting of 250 individuals of a cross between Kunmingxiaobaigu and a Japanese variety, Towada. Nine QTLs were detected by interval mapping in the F₂ population. The nine QTLs were distributed on chromosomes 1 (2 QTLs), 3, 4, 6 (2 QTLs), 7, 10 and 12. The total variance explained by these nine QTLs was 54.2%, while the variance explained by a single QTL ranged from 5.0% to 37.8%. The validity of four QTLs on chromosomes 3, 6 (2 QTLs) and 7 was confirmed by single-marker analysis in the F₃ population and they were designated as qRCT3, qRCT6a, qRCT6b and qRCT7, respectively. The QTLs qRCT3, qRCT6a and qRCT6b were reported here for the first time. The QTL qRCT7 displayed the largest effect, reaching a value of 20.6%.

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