Breeding Science
Online ISSN : 1347-3735
Print ISSN : 1344-7610
ISSN-L : 1344-7610
Research papers
Fine mapping of stable QTLs related to eating quality in rice (Oryza sativa L.) by CSSLs harboring small target chromosomal segments
Jingjing Li, Wenwei Zhang, Hongkai Wu, Tao Guo, Xiaolu Liu, Xiangyuan Wan, Jiansheng Jin, Than Thi Thu Hanh, Nguyen Thi Nhu Thoa, Mingjiang Chen, Shijia Liu, Liangming Chen, Xi Liu, Jiankang Wang, Huqu Zhai, Jianmin Wan
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Keywords: Eating and cooking quality, Rapid viscosity analyzer profiles, QTL, Chromosome segment subsitutution lines
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Supplementary material

2011 Volume 61 Issue 4 Pages 338-346

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Abstract

Amylose content (AC) and viscosity profile are primary indices for evaluating eating and cooking qualities of rice grain. Using chromosome segment substitution lines (CSSLs), previous studies identified a QTL cluster of genes for rice eating and cooking quality in the interval R727–G1149 on chromosome 8. In this study we report two QTLs for viscosity parameters, respectively controlling setback viscosity (SBV) and consistency viscosity (CSV), located in the same interval using rapid viscosity analyzer (RVA) profile as an indicator of eating quality. Previously reported QTL for AC was dissected into two components with opposite genetic effects. Of four QTLs, qCSV-8 and qAC-8-2 had stable genetic effects across three and four environments, respectively. qSBV-8, qCSV-8 and qAC-8-1 partly overlapped, but were separated from qAC-8-2. Based on data from an Affymetrix rice GeneChip, two genes related to starch biosynthesis at the qAC-8-2 locus were chosen for further quantitative expression analysis. Both genes showed enhanced expression in sub-CSSLs carrying the target qAC-8-2 allele, but not in sub-CSSLs without the target qAC-8-2 allele, indicating their possible role in rice quality determination. Molecular markers closely linked to the two stable QTL provide the opportunity for marker-assisted selection (MAS) in breeding high quality rice.

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© 2011 by JAPANESE SOCIETY OF BREEDING
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