All Issue

2014 Vol.23, Issue 1 Preview Page
Effect of Red LEDs during Healing and Acclimatization Process on the Survival Rate and Quality of Grafted Tomato Seedlings

토마토 접목묘의 순화과정에서 적색LED광조사가 접목 활착율과 묘소질에 미치는 영향

Ngoc-Thang Vu1
,
Young-Shik Kim2
,
Ho-min Kang1
,
Il-Seop Kim1*
넉탕 넉탕1
,
김 영식2
,
강 호민1
,
김 일섭1*
1Department of Horticulture, Kangwon National University, Chuncheon 200-701, Korea
2Department of Plant and Food Science, Sangmyung University, Cheonan 330-720, Korea
1강원대학교 원예학과
2상명대학교 식물식품공학과
*Corresponding author : kimilsop@kangwon.ac.kr
31 January 2014. pp. 43-49
PDF XML Citation
Abstract

This study was designed to determine the effect of red LEDs during healing and acclimatization process on the survival rate and quality of grafted tomato seedlings. Red LEDs and no light (darkness) were used for treating three rootstock cultivars, which are ‘B-Blocking’, ‘Kanbarune’, ‘High-power’ in healing room. Results showed that survival rates of grafted seedlings in red LEDs were higher than those in no light treatment. Significant variation on survival rates of rootstock cultivars was observed in no light treatment but there was not significant variation in red LEDs treatment. Light treatment also reduced the percentage of infected plants, except for the ‘Kanbarune’ cultivar. Seedling quality in red LEDs was better than that in no light treatment by improving growth parameters such as plant height, leaf length, leaf width, leaf area, fresh and dry weight of shoot and root. Light treatments and rootstock cultivars did not affect number of leaves, leaf chlorophyll value and T/R ratio of seedlings, but seedlings in red LEDs were significantly more compact than those in no light treatment. Moreover, the root morphology of seedlings such as total root surface area, total root length, and number of toot tips in red LEDs was also greater than that in no light treatment.

Keywords
growth parameters
infected plant
plug seedlings
rootstock
scion

본 연구는 토마토 플러그묘의 접목후, 순화과정에서 단기간 광조사 여부가 접목 활착율과 묘소질에 미치는 영향을 구명하기 위하여 실시 되었다. 공시 품종으로 ‘B-Blocking’, ‘Kanbarune’, ‘High-Power’의 3종 류 대목 품종에 방울토마토 ‘Choice’ 품종을 각각 접목한 뒤, 활착실에서 10일간 적색LED 광조사구와 무처리 (대조구)로 나누어 접목 활착율과 묘소질을 조사하였다. 적색 LED 광조사구의 접목 활착율은 대목품종에 관계 없이 무처리구에 비해 높았다. 광조사구 접목 활착율은 세 품종 모두 90% 이상으로 품종간의 유의성이 없었 으나, 무처리구의 접목 활착율은 35~72% 정도로 품종에 따른 차이가 매우 컸다. 활착기간 중 곰팡이병 이병 율도 ‘Kanbarune’를 제외하고 광조사구가 무처리구에 비해 저하하는 경향을 보였으나, 품종간 유의차는 나타나 지 않았다. 묘소질은 경경, 엽수, 엽록소 함량을 제외한 모든 생육지표에서 LED광 조사구가 우수한 결과를 나타내었으나, 처리에 따른 대목 품종간 유의차는 전반적으로 나타나지 않았다. 지하부 생육에서 평균 근경을 제외한 뿌리 표면적, 전 근장, root tip수는 광조사구가 무처리구에 비해 모든 품종에서 우세하였다.

키워드
생육지표
이병
플러그묘
접수
대목
References
1
Arsenault, J.L., S. Pouleur, C. Messier, and R. Guay. 1995. WinRHIZO, a root-measuring system with a unique overlap correction method. HortScience 30:906 (Abtract).
10.21273/HORTSCI.30.4.906D
2
Afshari, R.T., R. Fngoshtari, and S. Kalantari. 2011. Effect of light and different plant growth regulators on induction of callus growth in rapeseed (Brassica napus L.) genotypes. Plant Omics Journal 4:60-67.
3
Buck, J.W., W.B. Dong, and D.S. Mueller. 2010. Effect of light exposure on in vitro germination and germ tube growth of eight species of rust fungi. Mycologia. 102:1134-1140.
10.3852/09-28320943512
4
Davis, A.R., P. Perkins-Veazie, Y. Sakata, S. L'opez-Galarza, J.V. Maroto, S.G. Lee, Y.C. Huh, Z.Y. Sun, A. Miguel, R.K. Stephen, R. Cohen, and J.M. Lee. 2008. Cucurbit Grafting. Critical Reviews in Plant Sciences 27:50-74.
10.1080/07352680802053940
5
Goins, G.D., N.C. Yorio, M.M. Sanwo, and C.S. Brown. 1997. Photomorphogenesis, photosynthesis, and seed yield of wheat plants grown under red light-emitting diodes (LEDs) with without supplemental blue lighting. J. Exp. Bot. 48:1407-1413.
10.1093/jxb/48.7.140711541074
6
Jang, Y.A., E. Goto, Y. Ishigami, B. Mun, and C.H. Chun. 2011. Effect of light intensity and relative humidity on photosynthesis, growth and graft-take of grafted cucumber seedlings during healing and acclimatization. Hort. Enviro. Biotechnol. 52:331-338.
10.1007/s13580-011-0009-8
7
Jiao, Y., O.S. Lau, and X.W. Deng. 2007. Light-regulated transcriptional networks in higher plants. Nat. Rev. Genet. 8:217-230.
10.1038/nrg204917304247
8
Johkan, M., M. Oda, and G. Mori. 2008. Ascobic acid promotes graft-take in sweet pepper plants (Capsicum annuum L.). Scientia Horticulturae 116:343-347.
10.1016/j.scienta.2008.02.004
9
Johkan, M., K. Mitukuri, S. Yamasaki, G. Mori, and M. Oda. 2009. Causes of defoliation and low survival rate of grafted sweet pepper plants. Scientia Horticulturae 119:103-107.
10.1016/j.scienta.2008.07.015
10
Kim, I.S., C.H. Zhang, H.M. Kang, and B. Mackay. 2008. Control of stretching of cucumber and tomato plug seedlings using supplemental light. Hort. Environ. Biotechnol. 49:287-292.
11
Lee, J and M. Oda. 2003. Grafting of herbaceous vegetable and ornamental crops. Horticultural Reviews 28:61-124.
10.1002/9780470650851.ch2
12
Lee, J.M., C. Kubota, S.J. Tsao, Z. Bie, P. HoyosEchevarria, L. Morra, and M. Oda. 2010. Current status of vegetable grafting: Diffusion, grafting techniques, automation. Scientia Horticulturae 127:93-105.
10.1016/j.scienta.2010.08.003
13
Li, H., Z. Xu, and C. Tang. 2010. Effect of light-emitting diodes on growth and morphogenesis of upland cotton (Gossypiumhirsutum L.) plantles in vitro. Plant Cell Tissue Organ Cult. 103:155-163.
10.1007/s11240-010-9763-z
14
Moon, H.K. and A.M. Stomp. 1997. Effects of medium components and light on callus induction, growth, and frond regeneration in Lemna gibba (Duckweed). In Vitro Cell. Dev. Biol-Plant 33:20-25.
10.1007/s11627-997-0035-5
15
Moon, H.K., S.K. Park, Y.W. Kim, and C.S. Kim. 2006. Growth of Tsuru-rindo (Tripterospermum japonicum) cultured in vitro under various sources of light-emitting diode (LED) irradiation. J. Plant. Biol. 49:174-179.
10.1007/BF03031014
16
Neff, M.M., C. Fankhauser, and J. Chory. 2000. Light: an indicator of time and place. Genes & Develoment 14:257-271.
17
Nobuoka, T., T. Nishimoto, and K. Toi. 2005. Wind and light promote graft-take and growth of grafted tomato seedlings. J. Japan. Soc. Hort. Sci. 74:170-175.
10.2503/jjshs.74.170
18
Oda, M., K. Tsuji, and H. Sasaki. 1993. Effect of hypocotyls morphology on survival rate and growth of cucumber seedlings grafted on Cucurbita spp. J. Japan. Soc. Hort. Sci. 26:259-263.
19
Oda, M., K. Tsuji, K. Ichmura, and H. Sasaki. 1994. Factors affecting the survival of cucumber plants grafted on pumpkin plants by horizontal grafting at the hypocotyl level. Bul. Natl. Res. Inst. Veg. Ornamental Plants, Tea 9:51-60.
20
Oda, M. 1999. Grafting of vegetables to improve greenhouse production. Food and Fertilizer Technology Centre Extension Bulletin. 480:1-11.
21
Oda, M., M. Maruyama, and G. Mori. 2005. Water transfer at graft union of tomato plants grafted onto Solanum rootstocks. J. Japan. Soc. Hort. Sci. 74:458-463.
10.2503/jjshs.74.458
22
Ogata, T., Y. Kabashima, S. Shiozaki, and S. Horiuchi. 2005. Regeneration of the vascular bundle at the graft interface in auto-and hetero grafts with juvenile nucellar seedlings of satsuma mandarin, yuzu and trifoliate orange. J. Japan. Soc. Hort. Sci. 74:214-220.
10.2503/jjshs.74.214
23
Rivero. R.M., J.M. Ruiz, and L. Romero. 2003. Role of grafting in horticultural plants under stress conditions. Food, Agriculture & Environment. 1:70-74.
24
Salehi, R., A. Kashi, S.G. Lee, Y.C. Huh, J.M. Lee, M. Babalar, and M. Delshad. 2009. Assessing the survival and growth performance of Iranian melons to grafting onto Cucurbita rootstocks. Kor. J. Hort. Sci. Technol. 27:1-6.
25
Vu, N.T., C.H. Zhang, Z.H. Xu, Y.S. Kim, H.M. Kang, and I.S. Kim. 2013. Enhanced graft-take ratio and quality of grafted tomato seedlings by controlling temperature and humidity conditions. Protected Horticulture and Plant Factory 22:146-153.
10.12791/KSBEC.2013.22.2.146
26
Vu, N.T., Y.S. Kim, H.M. Kang, and I.S. Kim. 2014. Influence of short-term irradiation during pre- and post-grafting period on the graft-take ratio and quality of tomato seedlings. Hort. Environ. Biotechnol. 55:27-35.
10.1007/s13580-014-0115-5
27
Wang, H., M. Gu, J. Cui, K. Shi, Y. Zhou, and J. Yu. 2009. Effects of light quality on CO2 assimilation, chlorophyll-fluorescence quenching, expression of Calvin cycle genes and carbohydrate accumulation in Cucumis sativus. J. Photochem. Photobiol. B: Biol. 96:30-37.
10.1016/j.jphotobiol.2009.03.01019410482
28
Yu, H. and B.L. Ong. 2003. Effect of radiation quality on growth and photosynthesis of Acacia mangium seedlings. Photosynthetica. 41:349-355.
10.1023/B:PHOT.0000015458.11643.b2
29
Zhang, C., I.J. Chun, Y.C. Park, and I.S. Kim. 2003. Effects of timings and light intensities of supplemental red light on the growth characteristics of cucumber and tomato plug seedlings. J. Bio-Env. Con. 12:173-179.
Information
  • Publisher :The Korean Society for Bio-Environment Control
  • Publisher(Ko) :(사)한국생물환경조절학회
  • Journal Title :Protected Horticulture and Plant Factory
  • Journal Title(Ko) :시설원예ㆍ식물공장
  • Volume : 23
  • No :1
  • Pages :43-49
  • Received Date : 2014-01-23
  • Revised Date : 2014-02-24
  • Accepted Date : 2014-03-11
  • DOI :https://doi.org/10.12791/KSBEC.2014.23.1.043
Journal Informaiton Journal of Bio-Environment Control Journal of Bio-Environment Control
Online Submission
  • NRF
  • KOFST
  • crossref crossmark
  • crossref cited-by
  • crossref funder-registry
  • crosscheck
  • orcid
  • open access
  • ccl
Journal Informaiton Journal Informaiton - close