Production of GM-CSF Mediated by Cysteine Protease of Der f in Canine Keratinocytes

Tsuyoshi KIMURA; Machiko SEKIDO; Naoki CHIMURA; Sanae SHIBATA; Naho KONDO; Harumi KAMISHINA; Hiroaki KAMISHINA; Sadatoshi MAEDA

doi:10.1292/jvms.11-0522

Internal Medicine

Production of GM-CSF Mediated by Cysteine Protease of Der f in Canine Keratinocytes

Tsuyoshi KIMURA, Machiko SEKIDO, Naoki CHIMURA, Sanae SHIBATA, Naho KONDO, Harumi KAMISHINA, Hiroaki KAMISHINA, Sadatoshi MAEDA

Author information

Tsuyoshi KIMURA
The United Graduate School of Veterinary Sciences, Gifu University, 1–1 Yanagido, Gifu 501–1193, Japan
Machiko SEKIDO
Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1–1 Yanagido, Gifu 501–1193, Japan
Naoki CHIMURA
The United Graduate School of Veterinary Sciences, Gifu University, 1–1 Yanagido, Gifu 501–1193, Japan
Sanae SHIBATA
Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1–1 Yanagido, Gifu 501–1193, Japan
Naho KONDO
Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1–1 Yanagido, Gifu 501–1193, Japan
Harumi KAMISHINA
Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1–1 Yanagido, Gifu 501–1193, Japan
Hiroaki KAMISHINA
Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1–1 Yanagido, Gifu 501–1193, Japan
Sadatoshi MAEDA
Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1–1 Yanagido, Gifu 501–1193, Japan

Keywords: atopic dermatitis, canine, Der f, GM-CSF, keratinocytes

JOURNAL FREE ACCESS

2012 Volume 74 Issue 8 Pages 1033-1036

DOI https://doi.org/10.1292/jvms.11-0522

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Abstract

House dust mite (HDM) allergens are the most common allergens for induction of IgE-mediated hypersensitivity. Recently, epicutaneous sensitization with HDM allergens has been emphasized in the development of atopic dermatitis (AD) by producing various soluble factors in keratinocytes. Among the soluble factors, GM-CSF is a key molecule that activates Langerhans cells, antigen-presenting cells in the epidermis. In the present study, we investigated the effects of Dermatophagoides farinae (Der f) on GM-CSF production in a canine keratinocyte cell line, CPEK. CPEKs were found to produce GM-CSF upon stimulation by Der f. The GM-CSF production was suppressed by addition of a cysteine protease inhibitor. The present results suggest that cysteine protease-derived Der f may be an initiator of allergic inflammation by inducing the production of GM-CSF in keratinocytes.

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