We investigated urinary N-acetyl-β-D-glucosaminidase NAG (EC 3.2.1.30), γ-glutamyl transpeptidase γ-GTP (EC 2.3.2.2) and glycyl-prolyl dipeptidyl aminopeptidase GP-DAP (EC 3.4.14.5) in dogs with heartworm disease and renal failure. In the renal failure dogs, the NAG, γ-GTP and GP-DAP index were significantly higher than those in the healthy dogs. In the heartworm disease dogs with normal chest X-rays (HW I), none of the enzyme values was significantly different from those of the healthy controls. In the dogs with heartworm disease showing abnormal heart shadows on their chest X-rays (HW II), enzyme values were significantly higher than those in the healthy dogs (P<0.01) and the HW I dogs (P<0.01). Thus, these urinary enzymes tests are available for the early detection of renal disorders.