Molecular Identification of a Recent Type of Canine Distemper Virus in Japan by Restriction Fragment Length Polymorphism

Kenjiro OHASHI; Kiyoko IWATSUKI; Kazuya NAKAMURA; Takeshi MIKAMI; Chieko KAI

doi:10.1292/jvms.60.1209

Abstract

Restriction fragment length polymorphism analysis was used to differentiate recent field viruses of canine distemper virus (CDV) from vaccine strains. Virus genomes were amplified by using reverse transcriptase-polymerase chain reaction in part of the haemagglutinin gene. After digestion with EcoRV, the PCR products of recent field isolates were cut into two fragments that differ from the uncut form of old strains including all of vaccine strains. This method could be applied to fresh or stored brains, spleens and peripheral blood mononuclear cells of infected dogs. This molecular approach is useful for determining the causative agent of post-vaccinated CDV infection.

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この記事はクリエイティブ・コモンズ [表示 - 非営利 - 改変禁止 4.0 国際]ライセンスの下に提供されています。
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja

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