Establishment of the PCR System Specific to Salmonella spp. and Its Application for the Inspection of Food and Fecal Samples

Sou-ichi MAKINO; Hisao KURAZONO; Manas CHONGSANGUAM; Hideo HAYASHI; Hyeng-il CHEUN; Saori SUZUKI; Toshikazu SHIRAHATA

doi:10.1292/jvms.61.1245

Sou-ichi MAKINO, Hisao KURAZONO, Manas CHONGSANGUAM, Hideo HAYASHI, Hyeng-il CHEUN, Saori SUZUKI, Toshikazu SHIRAHATA

Author information

Sou-ichi MAKINO
Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan
Hisao KURAZONO
Department of Medical Technology, School of Health Sciences, Okayama University, Okayama 700-8558, Japan
Manas CHONGSANGUAM
Department of Microbiology, Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki 305-8557, Japan
Hideo HAYASHI
Department of Microbiology, Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki 305-8557, Japan
Hyeng-il CHEUN
Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan
Saori SUZUKI
Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan
Toshikazu SHIRAHATA
Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan

Keywords: food, PCR, salmonella

JOURNAL FREE ACCESS

1999 Volume 61 Issue 11 Pages 1245-1247

DOI https://doi.org/10.1292/jvms.61.1245

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Abstract

We established the PCR detection system specific to Salmonella species using Salmonella enterotoxin gene (stn). The detection limit was one bacterial cell per one gram of fecal and minced-meat samples using enrichment procedure by Tripticase soy broth or Salmonella enrichment broth, respectively. We concluded that this PCR system is useful for the practical application in the field of the public hygiene.

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