Staphylococcus hyicus subsp. hyicus was isolated from 97 (23.7%) out of 408 healthy pigs, from 55 (18.9%) out of 290 healthy chickens and from 6 (6.2%) out of 96 bovine udders, respectively. All of the isolates (n=204) were positive in Tween 80 hydrolysis, heat-stable DNase and hyaluronidase, but negative in·hemolysin, clumping factor and coagulase. In particular, most of them showed strong reactions in gelatin hydrolysis and in the fibrinolysin test. In these properties, there was no difference as to the origin of the isolates.