A survey of Prototheca bovis infection in dairy farms of the          Hubei province, China

Jialin LI; Xiabing CHEN; Erguang JIN; Guiqiang WANG; Lijun WU; Zhiyong SHAO; Pingmin WAN; Changmin HU; Junhua LI; Jie CHEN; Mingxing DING

doi:10.1292/jvms.21-0068

Pathology

A survey of Prototheca bovis infection in dairy farms of the Hubei province, China

Jialin LI, Xiabing CHEN, Erguang JIN, Guiqiang WANG, Lijun WU, Zhiyong SHAO, Pingmin WAN, Changmin HU, Junhua LI, Jie CHEN, Mingxing DING

Author information

Jialin LI
Institute of Animal Husbandry and Veterinary Science, Wuhan Academy of Agricultural Sciences, Wuhan 430208, China
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China
Xiabing CHEN
Institute of Animal Husbandry and Veterinary Science, Wuhan Academy of Agricultural Sciences, Wuhan 430208, China
Erguang JIN
Institute of Animal Husbandry and Veterinary Science, Wuhan Academy of Agricultural Sciences, Wuhan 430208, China
Guiqiang WANG
Livestock and Poultry Breeding Center of Hubei Province, Wuhan 430070, China
Lijun WU
Institute of Animal Husbandry and Veterinary Science, Wuhan Academy of Agricultural Sciences, Wuhan 430208, China
Zhiyong SHAO
Institute of Animal Husbandry and Veterinary Science, Wuhan Academy of Agricultural Sciences, Wuhan 430208, China
Pingmin WAN
Institute of Animal Husbandry and Veterinary Science, Wuhan Academy of Agricultural Sciences, Wuhan 430208, China
Changmin HU
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China
Junhua LI
Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
Jie CHEN
Institute of Animal Husbandry and Veterinary Science, Wuhan Academy of Agricultural Sciences, Wuhan 430208, China
Mingxing DING
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China

Keywords: bovine mastitis, dairy farm, histopathological change, pathogen identification, Prototheca bovis

JOURNAL OPEN ACCESS

2021 Volume 83 Issue 8 Pages 1248-1255

DOI https://doi.org/10.1292/jvms.21-0068

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Abstract

Bovine mastitis, caused by Prototheca bovis, has received much attention worldwide. To investigate the status of P. bovis infection in dairy farms of Hubei, we collected 1,158 milk samples and 90 environmental samples from 14 dairy farms of Hubei, China. The isolates were identified with traditional biological methods and molecular biological techniques, and their pathogenicity was tested through mice infection experiments. Isolates from 57 milk and 20 environmental samples were identified as P. bovis. The mice infection tests proved that the isolated P. bovis could cause mastitis in mice, manifesting as severe red swelling of the mammary glands. Histopathological analysis of tissue sections showed necrosis and nodules lesions formed in the infected mice mammary tissue, accompanied by macrophage and neutrophil infiltration. These results suggested the existence of pathogenic P. bovis in dairy farms of the Hubei province, China, with brewer’s grains and fresh feces possibly playing important roles in the spread of this disease.

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