Mitotic spindle positioning protein (MISP) deficiency exacerbates dextran          sulfate sodium (DSS)-induced colitis in mice

Koki HIURA; Takumi MARUYAMA; Masaki WATANABE; Kenta NAKANO; Tadashi OKAMURA; Hayato SASAKI; Nobuya SASAKI

doi:10.1292/jvms.22-0483

Laboratory Animal Science

Mitotic spindle positioning protein (MISP) deficiency exacerbates dextran sulfate sodium (DSS)-induced colitis in mice

Koki HIURA, Takumi MARUYAMA, Masaki WATANABE, Kenta NAKANO, Tadashi OKAMURA, Hayato SASAKI, Nobuya SASAKI

Author information

Koki HIURA
Laboratory of Laboratory Animal Science and Medicine, School of Veterinary Medicine, Kitasato University, Aomori, Japan
Takumi MARUYAMA
Laboratory of Laboratory Animal Science and Medicine, School of Veterinary Medicine, Kitasato University, Aomori, Japan
Department of Laboratory Animal Medicine, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan
Masaki WATANABE
Laboratory of Laboratory Animal Science and Medicine, School of Veterinary Medicine, Kitasato University, Aomori, Japan
Kenta NAKANO
Department of Laboratory Animal Medicine, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan
Tadashi OKAMURA
Department of Laboratory Animal Medicine, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan
Hayato SASAKI
Laboratory of Laboratory Animal Science and Medicine, School of Veterinary Medicine, Kitasato University, Aomori, Japan
Nobuya SASAKI
Laboratory of Laboratory Animal Science and Medicine, School of Veterinary Medicine, Kitasato University, Aomori, Japan

Keywords: dextran sulfate sodium-induced colitis, inflammatory bowel disease, inflammatory cytokines, mitotic spindle positioning protein

JOURNAL OPEN ACCESS

2023 Volume 85 Issue 2 Pages 167-174

DOI https://doi.org/10.1292/jvms.22-0483

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Abstract

Inflammatory bowel disease (IBD) is classified into two types: Crohn’s disease and ulcerative colitis. In IBD, the imbalance between the pro-inflammatory and anti-inflammatory cytokines prevents recovery from the inflammatory state, resulting in chronic inflammation in the colon. The mitotic spindle positioning protein (MISP) is localized to the apical membrane in the colon. In this study, we observed increased expression of MISP in the intestinal epithelial cells in dextran sulfate sodium (DSS)-induced colitis in mice. MISP-deficient mice receiving DSS showed significant exacerbation of colitis (e.g., weight loss, loss of the crypts). The intestinal epithelial cells of the MISP-deficient mice showed a trend towards decreased cell proliferation after DSS treatment. Reverse transcription followed by quantitative polymerase chain reaction revealed that the expression levels of Tgfb1, an anti-inflammatory cytokine, were significantly reduced in the colon of MISP-deficient mice compared with the wild-type mice regardless of DSS treatment. These findings indicate that MISP may play a role in the recovery of the colon after inflammation through its anti-inflammatory and proliferative activities, suggesting that MISP may be a new therapeutic target for IBD.

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