Journal of Sun Yat-Sen University(Medical Sciences). 2011, (01) Core

Mechanism and Effect of Tumor Necrosis Factor a on Expression of P-glycoprotein and Absorption of RisperidoneChinese Full Text

XU Li-ping1,XIE Yong-biao2*,ZHOU Zhi-ling2(1.Guangzhou Psychiatric Hospital,Guangzhou 510370,China;2.Guangdong General Hospital∥Guangdong Academy of Medical Sciences,Guangdong Mental Health Institute,Guangzhou 510080,China)

Abstract: 【Objective】To explore the effect and mechanism of tumor necrosis factor a（TNF-a） on the P-glycoprotein（P-gp） expression and the absorption of risperidone in brain capillary endothelium cells.【Methods】Mouse brain capillary endothelium cell line bEnd.3 was incubated with TNF-a,and then JNK phosphorylation was detected by western blot.After the cells were pre-treated with SP600125 for 1 h（DMSO as vehicle control） and added with TNF-a,P-gp expression was analyzed by Western blot.Finally,bEnd.3 cells were incubated with TNF-a for 24 h and then added with risperidone for another 24 h,the concentration of risperidone in medium supernatant was assayed by HPLC-MS.【Results】 TNF-a increased JNK phosphorylation in bEnd.3 cells.TNF-a enhanced the P-gp expression,which was inhibited by SP600125.And TNF-a increased the concentration of risperidone in medium supernatant in bEnd.3 cells（μg/L,1.17 ± 0.18 vs 0.86 ± 0.17,P = 0.010）.【Conclusion】 JNK pathway is involved in the process of TNF-a enhancing P-gp expression.TNF-a can decrease the absorption of risperidone in brain capillary endothelium cells.

Keywords：

TNF-a; P-glycoprotein; risperidone; blood-brain barrier;

DOI：
10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2011.0015
Series：
Subject：
Classification Code：
R741

Mobile Reading

Read on your phone instantly

Step 1
Scan QR Codes

"Mobile CNKI-CNKI Express" App

Step 2
Open“CNKI Express”

and click the scan icon in the upper left corner of the homepage.

Step 3
Scan QR Codes

Read this article on your phone.
HTML
CAJ Download
PDF Download

Contents

Mechanism and Effect of Tumor Necrosis Factor a on Expression of P-glycoprotein and Absorption of RisperidoneChinese Full Text

XU Li-ping1,XIE Yong-biao2*,ZHOU Zhi-ling2(1.Guangzhou Psychiatric Hospital,Guangzhou 510370,China;2.Guangdong General Hospital∥Guangdong Academy of Medical Sciences,Guangdong Mental Health Institute,Guangzhou 510080,China)

Read on your phone instantly

Citation Network

Related Literature