A new approach to Cas9-based genome editing in Aspergillus niger that is precise, efficient and selectable
Fig 1
Design and application of cDNA006 for disruption of albA.
(A) of cDNA006 contains pyrG flanked by 300bp repeats at 1000 bp homology arms to albA. After integration, pyrG is excised by homologous recombination in the presence of 5-FOA. (B) and (C) Phenotypes obtained before 5-FOA and after 5-FOA. (D) Representative sequence showing the integration of the stop codon at the albA locus in a white colony.