Creating clear and informative image-based figures for scientific publications
Fig 3
Selecting magnification and using insets.
(A) Magnification and display detail of images should permit readers to see features related to the main message that the image is intended to convey. This may be the organism, tissue, cell, or a subcellular level. Microscope images [18] show D. melanogaster ovary (A1), ovarian egg chamber cells (A2), and a detail in egg chamber cell nuclei (A3). (B) Insets or zoomed-in areas are useful when 2 different scales are needed to allow readers to see essential features. It is critical to indicate the origin of the inset in the full-scale image. Poor and clear examples are shown. Example images were created based on problems observed by reviewers. Images show B1, B2, B3, B5: Protostelium aurantium amoeba fed on germlings of Aspergillus fumigatus D141-GFP (green) fungal hyphae, dead fungal material stained with propidium iodide (red), and acidic compartments of amoeba marked with LysoTracker Blue DND-22 dye (blue); B4: Lendrum-stained human lung tissue (Haraszti, Public Health Image Library); B6: fossilized Orobates pabsti [19].