Re-visiting the tympanic membrane vicinity as core body temperature measurement site

Wui Keat Yeoh; Jason Kai Wei Lee; Hsueh Yee Lim; Chee Wee Gan; Wenyu Liang; Kok Kiong Tan

doi:10.1371/journal.pone.0174120

Abstract

Core body temperature (CBT) is an important and commonly used indicator of human health and endurance performance. A rise in baseline CBT can be attributed to an onset of flu, infection or even thermoregulatory failure when it becomes excessive. Sites which have been used for measurement of CBT include the pulmonary artery, the esophagus, the rectum and the tympanic membrane. Among them, the tympanic membrane is an attractive measurement site for CBT due to its unobtrusive nature and ease of measurement facilitated, especially when continuous CBT measurements are needed for monitoring such as during military, occupational and sporting settings. However, to-date, there are still polarizing views on the suitability of tympanic membrane as a CBT site. This paper will revisit a number of key unresolved issues in the literature and also presents, for the first time, a benchmark of the middle ear temperature against temperature measurements from other sites. Results from experiments carried out on human and primate subjects will be presented to draw a fresh set of insights against the backdrop of hypotheses and controversies.

Citation: Yeoh WK, Lee JKW, Lim HY, Gan CW, Liang W, Tan KK (2017) Re-visiting the tympanic membrane vicinity as core body temperature measurement site. PLoS ONE 12(4): e0174120. https://doi.org/10.1371/journal.pone.0174120

Editor: Andrej A. Romanovsky, St. Joseph’s Hospital and Medical Center, UNITED STATES

Received: July 7, 2016; Accepted: March 3, 2017; Published: April 17, 2017

Copyright: © 2017 Yeoh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Data Availability: All relevant data are within the paper and its Supporting Information files.

Funding: The author(s) received no specific funding for this work.

Competing interests: The authors have declared that no competing interests exist.

Introduction

Core body temperature (CBT) is an important and commonly used indicator of the human health and endurance performance. The characteristics of CBT and the ideal measurement site have attracted extensive research in both clinical and the physiological domains. CBT is generated from the well-perfused tissues of the vital organs [1, 2] and thus, it remains relatively stable since heat distribution within these tissues is attained at a fast rate. As a result, the CBT of a healthy human body rarely differs and it is very close to the temperature of blood flowing in the pulmonary artery [3, 4]. Thus, the blood temperature of the pulmonary artery (T_pa) is often taken as the gold standard in CBT measurement [3–6]. However, the measurement of T_pa is an invasive and high risk procedure, requiring the insertion of a pulmonary artery catheter. Thus, T_pa measurement is mainly restricted to critical care monitoring within a hospital environment [6], wherein cardiac or critically ill patients are being monitored round the clock.

Apart from the pulmonary artery, the esophagus and rectum are other sites to measure CBT [6–9]. The esophagus site requires rather invasive process to access, while the rectum site is rather inconvenient in term of its accessibility. These sites are inherently less risky compared to the pulmonary artery and certain considerations have to be observed in order to obtain accurate and consistent temperature measurements. Esophageal temperature (T_es) is accurate when the probe is positioned at the lower fourth of the esophagus which is closer to the heart and aorta. If the probe is located too high up in the esophagus, the reading will be affected by tracheal air due to the breathing process [10, 11]. T_es can be a good candidate for the CBT due to its responsiveness which is on-par with T_pa [12], but due to the sensitivity to proper probe placement, the distress caused to patient during measurement and the associated cost and time, the esophagus is also not a common site for CBT.

Rectal Temperature (T_rm) is considered as an indicator of deep tissue and critical tissue temperatures [10]. The large tissue mass surrounding the rectum provides for a stable temperature reading that is shielded from the surrounding environmental temperature [10, 13]. However, T_rm is not suitable as an indicator of CBT when there is a need to pick up rapid changes in the CBT [13, 14]. When the human’s body is at thermal equilibrium, T_rm is a reliable substitute of the CBT. But when the CBT is undergoing a transient period, T_rm is sluggish to pick it up as it exhibits slow response to changes in heat input and losses, and follows rather than leads the body’s thermoregulatory reactions [13, 14]. Hence, T_rm is limited in the ability to respond to thermal transients involving sudden or abrupt changes to CBT. For a critically ill patient, changes in CBT is crucial to determine the next treatment protocol [1, 3]. Such limitations have led to the suitability of T_rm to determine CBT being repeatedly questioned, though till now, the rectum is still a widely used CBT site due to the relatively efficient and stable measurements.

The aforementioned sites are not amenable towards general use or temperature monitoring in active personnel. Generally, all of them pose a certain amount of risk and discomfort (as well as embarrassment (e.g., T_rm measurement)) from the user perspectives and they can incur rather high costs. The ideal CBT site has, by far, remained elusive to-date. Thus, research continues to be undertaken to explore alternative temperature measurement sites which are conducive for general and continuous monitoring purposes, and are reliable sufficiently as an indicator of the CBT. One such site is the tympanic membrane. Tympanic membrane temperature (T_tm) measurement via infrared thermometry has been touted as a potential replacement for the invasive temperature measurement procedures discussed. The safe, least invasive and arguably most comfortable way of measuring T_tm, however, is not yet a de-facto standard to-date; not when there are still unresolved issues pertaining to its accuracy and stability relative to measurements from other sites [2, 5, 11, 15].

Benzinger [16–18] first demonstrated the feasibility of T_tm measurement as an indicator of CBT using a thermocouple temperature probe engaging the surface of a tympanic membrane with the ear canal sealed off from the environment. Benzinger hypothesized that the tympanic membrane, being in close proximity to the hypothalamus and to the internal carotid artery (and since the internal carotid arterial blood flow perfuses the tympanic membrane), is most suitable for accurate indication of CBT. To support his hypothesis, Benzinger produced measurements of T_tm obtained from his probe and measurement approach, showed them to be stable, reproducible and responsive to thermal stresses of various kinds, and also demonstrated T_tm to be more responsive than T_rm in indicating CBT.

On the other hand, subsequent studies by McCaffrey et al [19] and Nielsen [20] indicated that head cooling, particularly when the cooling is directed at the facial area, decreases T_tm. McCaffrey et al showed that by heating and cooling localized regions of the head, T_tm of human subjects were non-proportionally affected by changes in the skin temperature of the head. These results meant T_tm, by itself, is not a good indication of CBT since it is affected by other variables.

However, Brinnel and Cabanac [21], and Sato et al [22] furnished data to show that T_tm is still reliable for this purpose if the measurement spot on the tympanic membrane is chosen with care and an adequately directed probe is used to acquire the measurements. Brinnel and Cabanac suggested that the lower anterior quarter of the tympanic membrane (Fig 1) has a higher temperature on the surface of the tympanic membrane and a temperature measurement from a point in this region is least sensitive to head cooling. Sato et al performed similar experiments as Brinnel and Cabanac, and they determined the highest measured temperature spot on the tympanic membrane with a trial and error approach of probe positioning until the hotspot is found. They produced results, with the probe engaging this spot on the tympanic membrane, showing the measurements obtained were least sensitive to the effects of head cooling. However, they did not define the point of measurement.

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Fig 1. Measurement spot on the tympanic membrane.

Tympanic membrane of the right ear showing a temperature measurement spot at the lower anterior quarter (pointed by the arrow).

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These works were done with the probe engaging the surface of the tympanic membrane which is neither practical nor safe from perforation of the tympanic membrane when taking measurements from awake patients, especially when measurements are required continually. However, the need to engage the tympanic membrane can be understood since the surface of the tympanic membrane is not flat but conically shaped and a physical contact is essential to ensure that the measurements from all sites on the surface can be compared in a fair manner. The ideal spot which can be easily located for measurement has also not been clearly specified and substantiated by previous studies.

This paper was motivated by the above-mentioned issues and gaps in allowing T_tm to be used as an inference to CBT. In addition, the paper sought to explore, to the best of our knowledge for the first time, an alternate CBT measurement site in the middle ear cavity. Being located at the inner side of the tympanic membrane, we aimed to shed light on the existing gaps in the use of the ear as a CBT site. The middle ear cavity resembles a rectangular chamber with four walls, a ceiling and a floor. The lateral wall being the tympanic membrane, the ceiling is a thin plate of bony structure that separates the chamber from the cranial cavity and the brain, and the floor is another thin bony plate that separates the chamber from the jugular vein and the carotid artery. The posterior wall partly separates the chamber from the mastoid antrum. The anterior wall is the opening of the eustachian tube, which connects the chamber with the nasopharynx. The medial wall separates the chamber from the inner ear [23]. This site selection is appropriate since the middle ear cavity is very near to the brain (ceiling), jugular vein and carotid artery (floor), and it is surrounded by highly perfused tissues. The temperature at this cavity (T_me) can potentially reflects a stable temperature to infer CBT. The reason for the lack of effort in the exploration of this site may be attributed to the difficulty in the access to the middle ear. However, in recent years, advances in medical surgery have led to the simplification of the procedure to carry out a ventilation tube insertion on the tympanic membrane to an office-based procedure [24, 25] without any need for general anesthesia. A channel can thus be readily created to access the middle ear via the tube for temperature measurements. An alternate access path is also possible through the Eustachian tube [26].

Specifically, we aimed to: (1) examine the surface temperature distribution at a small but within constant proximity from the tympanic membrane, and the identification of a clear landmark for the measurement of T_tm. The results can be potentially useful in the design of tympanic membrane thermometers to infer close readings to CBT in the face of environmental cooling. (2) benchmark the middle ear temperature against current CBT sites (including the tympanic membrane), with a side objective to observe if T_me shows characteristics which can be observed from T_tm. The results can potentially be useful towards further exploration of the middle ear as a CBT site. Apart from these two issues, we verified the cooling effects on the measurements of T_tm, the relative responsiveness and repeatability of measurements in T_es and T_rm, and the correlation among temperatures from the various sites.

We applied for the necessary approval from the SingHealth Institutional Animal Care and Use Committee (IACUC) prior to the commencement of the experiments involving a live animal subject. The IACUC reviewed the experiments procedures and concluded that the experiments were proper and humane, hence we were given the approval (REF:2012/SHS/712) to proceed with the experiments as planned.

The paper is organized as follows. In Exploration Focal Points, we will lay down the two main issues to be explored in the paper, the background behind them and the potential outcomes from the investigations set forth. In the Design of Experiment section, we will highlight the experiments and procedures, subjects and instruments used. In the Results and Discussions section, the results from the experiments will be presented and discussed. Finally, conclusions will be drawn in the last section.

Exploration focal points

It should be acknowledged at the outset that the study of CBT is an extensive and challenging one. It is not realistic to expect an exhaustive and comprehensive set of results from any single and specific work due to the complexity and the nature of the human body and the constituent organs, as well as the dynamic interactions with endogenous and exogenous influence. The paper seeks to focus on two specific issues (1. Temperature Uniformity in the Tympanic Membrane Vicinity, and 2. Middle Ear Cavity as CBT Site) to revisit the tympanic membrane vicinity as a potential CBT site. These two issues will be clearly formulated with respect to the background work and gaps, and the exploration approaches projected to align with the experiments and results which will be presented in the ensuing subsections.

Temperature uniformity in the tympanic membrane vicinity and measurement point selection

While the outer ear is an excellent site for temperature measurements, it is well-known that the readings from commercial ear thermometers are lacking in repeatability and accuracy [2, 5, 11, 15]. One main reason for this phenomenon is the different ear anatomy from one person to the next, and thus the difficulty in the design of a general-purpose probe which can be inserted into the outer ear with good sealing from the environment and with the probe distal end well directed at a desirable measurement point. This has prompted us to first investigate on the ideal measurement point or region within the ear. It is important to orientate the probe to a specific ear anatomy. Previous studies [19, 20] have shown that arbitrary measurement points on the tympanic membrane can lead to measurements that are very sensitive to external effects such as face cooling.

Brinnel and Cabanac [21], and Sato et al [22] proposed probes engaging the tympanic membrane surface at restricted points on the tympanic membrane, but the exact location of these points were not clear nor in concurrence. Brinnel and Cabanac suggested a measurement spot at the lower anterior quarter of the tympanic membrane would have the highest temperature and is least sensitive to head or face cooling. Sato et al also sought to find the hottest spot on the tympanic membrane with trial and error by positioning the probe until the hotspot was found. The probe was engaged to the tympanic membrane at all the measurement points so that the results can be interpreted on a level field with respect to the convective heat losses which will be minimized with contact. This common requirement to physically engage the tympanic membrane is reasonable and sound since the tympanic membrane is not flat but conically shaped. Engaging the tympanic membrane is a way to ensure all temperatures are taken at the same proximity to the tympanic membrane in order to fairly select the hottest spot. However, in practice, it is unrealistic and unsafe to take T_tm measurement this way, with the probe engaging the tympanic membrane in a conscious individual.

The selection of the hottest spot is a common recommendation from the above mentioned studies [21, 22], though Sato et al did not put a boundary to the spot while Brinnel and Cabanac suggested the lower anterior quarter shown in Fig 1. Choosing the spot with the highest temperature is reasonable too since this is likely to be the point where it is most stable against environmental influence. However, it is noteworthy that these experiments were conducted in settings wherein the environment temperature was lower than the CBT. Should the opposite happen, heat flow would change direction and we could be seeking the coolest point instead when the body seeks to thermoregulate and maintain CBT against external heat stress.

Preliminary tests and simulation were done to explore the uniformity of temperature distribution on the tympanic membrane. A non-contact thermopile sensors array (TSA) was used to measure the surface temperature of the tympanic membrane of a healthy volunteer. A speculum of an appropriate size was first inserted into the ear canal to straighten it. The TSA was positioned within the speculum to measure the temperature of the exposed tympanic membrane. The Melexis MLX90620 TSA was used which contains 64 infrared pixels arranged in a 16 × 4 rectangle area with an optical field of view of 60° to detect far infrared (FIR) induced thermal radiation. It can measure temperatures without making direct contact with the tympanic membrane. Each pixel has a 16-bit temperature measurement resolution, and all pixels have been factory calibrated with an accuracy of ±1.5°C for temperature measurements within the range of 0 to 50°C. Fig 2(a) depicts the measurement results showing the flattened temperature uniformity map of the ear canal space wherein the dotted line rectangle contains the temperature measurements of the tympanic membrane. Here, our interest is to show up the temperature deviation across the tympanic membrane and the canal area. Thus, different shades of color were used represent the relative temperatures. Shades of blue represents the lowest temperature, followed by green, yellow and red (highest temperature). In this measurement, due to the anatomical constraint, only half of the tympanic membrane was exposed to the direct line of sight of the TSA. The measured temperatures are non-uniform across the view window including the tympanic membrane. The measurement results in Fig 2(b) show the effects of face cooling. In general, with the cooling, it can be observed that the tympanic membrane temperatures as well as those at the surrounding ear canal walls lowered in response to the cooling, though not at the same proportion. The region with the highest temperature is observed (and marked as “TMA”) within the demarcated tympanic membrane area. The “TMA” area in Fig 2(b) is observed to be less sensitive to the effects of face cooling.

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Fig 2. Temperature uniformity maps.

Temperature uniformity maps of the left ear: (a) without face cooling and (b) without face cooling.

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Finite Element Analysis (FEA) simulation was performed on a model of a human ear canal and middle ear compartment (Fig 3). The tissues and bones parameters were selected to closely match those of reported human tissues and bones at these two sites. The purpose of the FEA is to verify the temperature non-uniformity on the tympanic membrane surface and at 1mm above the surface. The tympanic membrane was modeled as a circular disc of 9mm in diameter and 0.1mm thick with malleus, incus and stapes bones attached from behind, and the front attached to the ear canal. The ear canal was modeled as an air filled hollow cylinder of 9mm in diameter and 25mm in length. The middle ear was modeled as a rectangular compartment with six walls, and the tympanic membrane was attached to the lateral wall. It is assumed that the superior wall is near to the brain, the inferior wall near to the carotid artery supplies the main heat source to the middle ear compartment and the perfused tissues surrounding the middle ear and the ear canal serve as heat sources. In the FEA simulation, the initial steady state temperatures of the malleus, incus and stapes bones location within the enclosed middle ear were correlated to the heat emitted from the brain, the carotid artery and the perfused tissues.

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Fig 3. Human ear model.

Human Ear Model used in Finite Element Analysis.

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The tympanic membrane temperature uniformity map of the tympanic membrane surface is shown in Fig 4(a). From this simulation, we observe the area near to the malleus bone registered the highest temperature. This may indicate that the point of maximum temperature may not necessarily always be sited in the lower anterior quadrant [21] and depending on individual ear anatomy may lie elsewhere. The temperature uniformity map at 1mm above the tympanic membrane surface is depicted in Fig 4(b). Here, while the temperatures may be lower generally due to convective losses, the uniformity has remained rather consistent with the that at the tympanic membrane surface (Fig 4(a)). It may not be necessary to physically engage the tympanic membrane for the purpose of locating the highest temperature point.

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Fig 4. FEA results.

FEA showing temperature uniformity: (a) on the tympanic membrane and (b) at 1mm above the tympanic membrane.

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With these observations, we next aimed to experimentally obtain temperatures at accessible and potential points on the tympanic membrane without a physical engagement of the tympanic membrane, but maintaining the same small but uniform proximity from probe to the tympanic membrane, using a modified probe capable of proximity sensing. We would verify the temperature uniformity of these points on the tympanic membrane, and from the results, select the most appropriate point for measurement. As potential candidates, we chose a point in front of the malleus bone (Point A), a point in the lower anterior quarter (Point B) and a point at the distal end of the ear canal nearest to the tympanic membrane (Point C) (Fig 5). Point A was chosen because the tissue near to the malleus is thicker and accordingly a likelihood of a higher concentration of blood vessels [27]. Our conjecture, following the FEA analysis, is that this point has a larger thermal mass than those further away from the malleus bone. Point B is at the lower anterior quarter of the tympanic membrane. It was selected as suggested by Brinnel and Cabanac [21]. Point C lies on the canal just adjoining to the tympanic membrane. It was selected to serve as a comparison to A and B measurements, and it would represent the point at which temperature is typically taken with a wrongly directed ear thermometer.

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Fig 5. Temperature measurement points for Experiment 1.

Tympanic membrane of the right ear with temperature measuring points labeled as A, B and C.

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Experiment 1 was conducted to enable this investigation. In Experiment 2 on a sedated primate, we verified the effects of facial cooling on T_tm by measuring T_tm in both ears but fanning only one side of the face. The effects on other CBT sites, including the esophagus and rectum, would also be studied.

The outcomes from this investigation will be beneficial in the design of ear thermometers to infer a temperature that is most representative of CBT.

Middle ear cavity as CBT site

As explained in the Introduction, the middle ear cavity is closest to the brain than the other CBT sites. It holds unexplored potential to be an usable CBT measurement site. Till recently, accessing the middle ear space is challenging and it is not a natural approach for the purpose of taking a temperature reading. However, with the advent in technology, it is now a simple office-based procedure [24, 25] to incise a small opening on the tympanic membrane and to maintain a small opening with a ventilation tube. An alternate access to the middle ear space is via the Eustachian tube which can be facilitated too in an office setting.

An experiment (Experiment 3) on a sedated primate was completed to log temperatures from the middle ear and other CBT sites (esophagus, rectum) under passive hyperthermia. In addition, it is known that the administration of general anesthesia will lower CBT [1, 9]. An interesting time window, when general anesthesia is removed during the experiment, is available for scrutiny into the relative responsiveness of the CBT candidates following a change in CBT due to the influence of general anesthesia.

From the results of these tests in Experiment 3, we compared the temperature measured at two different points on the tympanic membrane against the temperature measured from the middle ear (presumed as the CBT benchmark) to show the sensitivity of the T_tm measurements with respect to locations.

Design of experiments

The setup of the experiments and the procedures prescribed are highlighted to provide the background in the interpretation of the experiments and results subsequently.

Equipment

The key equipment used in these experiments include the temperature probes and the custom heating chamber. The details of these equipment are given in the ensuing subsections.

Temperature probes.

Clinical temperature probes (Tympanic Membrane: D-TM1A, Esophagus: D-OS4A, Rectum: D-RB3A and Middle Ear: D-N1205) supplied by Exacon Scientific A/S were used in the experiments. The thermistor-based probes for measuring T_tm, T_me, T_es and T_rm have a consistent measurement accuracy of ±0.1°C for a temperature range between 25 and 50°C. The National Instruments Data Acquisition (NI-DAQ) system was used with a custom-built temperature recording software for automated temperature data acquisition from the attached temperature probes. The sampling interval was set to 0.1 second. The probes were first calibrated by immersing them in a glass container with 500ml of distill water at an initial temperature of 30°C. The glass container was placed in a heating chamber with the interior air temperature set to 65°C. The water was slowly heated within the chamber until it reached the target temperature of 50°C. The variations in temperature were logged for each of the probes. Using the temperature profile of the T_rm probe as a reference, the calibration curves were derived for each of the temperature probes to ensure they were equalized to give homogeneous response in the experiments.

In Experiment 1, it was a deliberate intent that, unlike previous work, the probe would not physically engage the tympanic membrane at these points, but at a very small but consistent proximity to them to observe the temperature difference between them in a way more typical of how a tympanic membrane temperature is taken. Modification to the T_tm probe was done to achieve this. It was retrofitted with a Keyence Reflective Fibre Unit (Model: FU-46 attached to a matching Fibre Amplifier Model: FS-N11N) at 1mm behind the sensing tip (Fig 6). This allowed the handler of the probe to be prompted when the probe was brought to a distance of <1mm from the defined measurement point for temperature measurement.

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Fig 6. Modified clinical tympanic membrane probe.

Clinical tympanic membrane probe supplied by Exacon Scientific A/S retrofitted with a Keyence Reflective Fibre Unit for use in Experiment 1.

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Heat chamber and probes setup.

A custom-built heat chamber similar to a small size incubator was designed and fabricated for the purpose of elevating the primate’s body temperature (full body passive hyperthermia) under controlled environment (Fig 7) in Experiments 2 and 3. The primate was sedated with general anesthesia in the chamber with the body from the neck downwards fully enclosed within the chamber. Only the head was exposed to external ambient temperature (T_am) of the clinic. The trapped air in the interior of the chamber was heated with a 300W electric filament heater to reach a chamber temperature (T_ch = 65°C). Fans, controlled by an electronic thermostat, were used to circulate the heated air in-order to ensure temperature was distributed evenly T_ch ± 2°C within the chamber. T_ch was maintained until the primate’s esophageal or rectal temperature reached 42°C (the limit approved by IACUC), after which the heater was turned off before the chamber was opened to begin the cool-down process. Temperature probes were inserted into the primate’s body at defined measurement locations to measure the temperatures at these sites. Additional probes were used to measure T_ch and T_am. Measurements were sampled every 0.1 second interval at each of the temperature probes. The raw temperature data sampled from the temperature probes were processed to remove noises and calibrated to ensure uniform responses.

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Fig 7. Custom heating chamber.

Custom heating chamber constructed for use in Experiments 2–3.

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Procedures

The procedures behind the three controlled experiments are elaborated in the ensuing subsections.

Experiment 1.

In Experiment 1, temperature measurements using the probe (retrofitted with proximity sensing) were taken at the defined points (A, B and C) on the tympanic membrane surface of a healthy adult human volunteer.

The ear canal has a diameter of about 5–8 mm in adults. Even though the modified probe has an outer diameter of 2.3mm, there is limited manoeuvring space to accurately access more points and not cause distress to the volunteer. The three points defined earlier in the Exploration Focal Points section were located for measurements; Point A in the vicinity of the malleus bone, Point B in the lower anterior quarter, and Point C at the distal end of the ear canal closest to the tympanic membrane as shown in Fig 5.

Under the view of a surgical microscope in a clinical setting with an ambient temperature of 22.5°C, an otolaryngologist guided the modified probe to the three points. The proximity sensor provided an alert when the probe reached a preset value of 1mm from the tympanic membrane. The same proximity was observed for measurements at all the three points. Steady state temperatures were logged after the measurements have stabilized.

Experiment 2.

Experiment 2 was conducted to observe the effects of facial cooling on T_tm, T_es and T_rm measurements. Probes were inserted by a veterinarian into a sedated primate to acquire real-time temperatures at these sites when it is placed within the heat chamber set to a temperature of T_ch = 65°C. At the mid-point during the passive hyperthermia process, convection skin cooling was initiated at the head area near to the left ear (left face fanning) by blowing cold air at T_am = 20°C with a small electric fan. The head area near to the right ear was shielded from the cooling process. Four temperature probes were used throughout the experiment: (a) Left tympanic membrane probe touching the left tympanic membrane’s surface for measuring T_tm,L, (b) Right tympanic membrane probe touching the right tympanic membrane’s surface for measuring T_tm,R, (c) Rectal probe was inserted via the anal sphincter for measuring T_rm, and (d) Esophageal probe was inserted into the esophagus for measuring T_es.

Experiment 3.

In Experiment 3, the temperature of middle ear cavity was measured, for the first time, on its suitability as an inference to CBT. There were two parts to this experiment: Experiment 3A and Experiment 3B. In Experiment 3A, T_me was benchmarked against T_tm and T_rm with the sedated primate undergoing full body hyperthermia within the chamber set to T_ch = 65°C. Note that Experiment 2 and Experiment 3 were carried out on the same primate but the trials were separated by two months in order to allow sufficient time for primate to recover. Three temperature probes were used: (a) A needle probe to perforate the left tympanic membrane to reach the middle ear cavity just in front of the malleus bone for measuring T_me, (b) Tympanic membrane probe engaging the right tympanic membrane’s surface to measure T_tm, and (c) rectal probe inserted via the anal sphincter to measuring T_rm.

Experiment 3B presented an ideal opportunity in the experiment setting to trigger a change in the CBT and to observe the relative measurements at various sites including the middle ear. The CBT trigger (approved by IACUC) was achieved by halting the administration of general anesthesia for 15 minutes, 2 hours and 51 minutes into the experiment. An additional probe was inserted into the primate’s esophagus to measure T_es. Thus, a total of four temperature probes were used to each measure T_me, T_tm, T_es and T_rm throughout this experiment.

Two different spots were selected on the tympanic membrane for the measurements of T_tm in Experiments 3A and 3B. By comparing with T_me, the sensitivity of the measurement points on T_tm could also be observed.

Ethics consideration

Experiment 1 required non-contact temperature measurements from the surfaces of the ear canal and tympanic membrane of a healthy adult human. This procedure was conducted on one of the researcher who also co-authored this paper. The researcher has volunteered and consented to be part of the experiment. Approval from the Institutional Review Board (IRB) was not sought or required as the volunteer is a co-author (with no conflict of interest) and no other volunteers who were not part of the research were engaged for the experiment. The temperature measurements were performed without any physical contacts to the volunteer’s body surface by an Otolaryngologist (co-author), with no physical risk of ear injury.

A live adult primate (breed: Cynomolgus monkey, age: 6 years, weight: 5kg) was engaged for two rounds of experiments involving: (a) elevation of primate body temperature up to 42°C, (b) measurement of temperatures from the rectum and esophagus, (c) measurement of temperature from the surface of the tympanic membrane, and (d) measurement of temperature from middle ear cavity. Approval (REF: 2012/SHS/712) from SingHealth IACUC was obtained for the purpose of conducting Experiments 2 and 3 involving a live adult primate. The experiments were conducted in SingHealth Experimental Medicine Center animal facility that conformed to the National Advisory Committee for Laboratory Animal Research (NACLAR) guidelines.

Throughout each experiment runtime, the primate was sedated with inhaled Isoflurane anesthetic agent (general anesthesia) and its vital signs were closely monitored by a veterinarian. The primate was provided with appropriate medical treatments and adequate nutrition. Sufficiently long rest duration was also enforced on the primate in-order to allow it to rest and recuperate before being subjected to another round of experiment of the similar nature.

Results and discussions

The results from the experiments are interpreted and analyzed according to the two focal points of the paper.

Temperature uniformity in the tympanic membrane vicinity and measurement point selection

The ambient temperature T_am, T_tm,A at point A and T_tm,B at point B of the tympanic membrane, as well as the ear canal temperature T_cn at Point C were measured as 22.5, 37.0, 36.4 and 36.1°C respectively at steady state. As suggested by Sato et al [22], the spot with the higher temperature is the preferred spot since this point is likely to be the least sensitive to ambient disturbances. An interesting observation from the results is that Point B registered a lower temperature than Point A, and Point B is within the lower anterior quadrant of the tympanic membrane suggested by Brinnel and Cabanac [21] to capture a temperature measurement. These results show that arbitrary point selected in this region may not necessarily return a highest temperature compared to other points on the tympanic membrane surface. If such a point does exist in this quadrant, it may require a search over the zone to pick up the highest temperature point. On the other hand, Point A which is at the malleus bone can be more easily located to focus the probe at. This is also intuitive since Point A is at the part of the tympanic membrane which is thicker compared to the lower anterior quadrant and that may translate into a higher thermal resistance against ambient disturbances, as the FEA also showed.

The stability of T_tm,A was additionally studied for resilience against temperature rise at the skin from peripheral vasodilation. The subject did a very short exercise of push-ups and sit-ups lasting at a leisurely pace over not more than 5 minutes, to induce metabolism to increase at the legs, hands and stomach muscles. Over this short duration, the body’s thermoregulatory system is expected to seek a balance between the maintenance of the body’s core temperature and the release of perspiration to cool the body. Thus, vasodilation will occur to cause an increase in blood flow to transport excess heat generated from affected muscle tissues to the skin. Measurement taken at the canal (Point C) registered a rise to 36.7°C while T_tm,A remained resilient at the same 37.0°C.

The sensitivity and effects of facial cooling on the temperatures from the various measurement sites can be observed from the results of Experiment 2. The initial temperature readouts from the temperature probes attached to the primate’s body were: (1) Rectum, T_rm = 37.2°C, (2) Esophagus, T_es = 36.8°C, (3) Left-Tympanic Membrane surface, T_tm,L = 36.2°C, and (4) Right-Tympanic Membrane surface T_tm,R = 36.1°C. 45 minutes into the experiment, convection skin cooling was introduced at the head area near to the left ear (left face fanning). Fig 8 shows the collected temperature readings at the four measurements points on the primate’s body. For clarity of view and interpretation, we split the plot into the two different test scenarios: (1) X—Full Body Passive Hyperthermia only, and (2) Y—Full Body Passive Hyperthermia with Left Face Fanning. T_es served as the reference temperature to compare against the T_tm,L and T_tm,R.

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Fig 8. Temperature measurements results from Experiment 2.

Primate body temperature measurements with Esophageal, Rectal and Tympanic Membrane (Left TM and Right TM) Temperatures plots. Experiment results are split into two test scenarios for Experiment 2: (1) X—Full Body Hyperthermia, and (2) Y—Full Body Hyperthermia with Left Face Fanning.

https://doi.org/10.1371/journal.pone.0174120.g008

The relationships between T_tm,L and T_es, and between T_tm,R and T_es over the full time period of Experiment 2 (X + Y test scenario) can be explained by using the correlation and Bland-Altman analyses. The details of the analyses are furnished in Figs A1–A6 in S1 File. Correlation analyses indicate strong correlations between T_es and both T_tm,L (Pearson correlation coefficient (r) = 0.982, Sum of Squared Error (SSE) = 0.25, Probability in support of a null hypothesis (p)<0.001) and T_tm,R (r = 0.996, SSE = 0.15, p < 0.001). Bland-Altman analyses show the mean differences (MD) between these temperatures (T_tm,L,T_es) and (T_tm,R,T_es) are −1.408°C and −0.963°C, and the 1.96SD are −0.768°C and −0.606°C, respectively. The dynamic response of T_es, T_tm,L and T_tm,R are stable and steadily increasing with chamber heating. Both T_tm,L and T_tm,R indicate very strong correlations with T_es with r → 1 even with left face fanning throughout the experiment though the correlation is higher on the right side. However, the respective SSE and MD vary with SSE = 0.25 and MD = 1.408°C for T_tm,L, and SSE = 0.15 and MD = 0.963°C for T_tm,R. Notably, there is a 40% in reduction of SSE, and 31.6% in reduction of |MD| for T_tm,R, compared to T_tm,L. Thus, the effects of face fanning on T_tm can be observed from this analysis.

The earlier analysis was done to compare T_tm in both ears with one side of the face undergoing fanning. It will be interesting too to examine the correlation on the side with fanning, before and after the onset of fanning. This analysis will show too if it is feasible to adopt a single CBT inference model for T_tm in the presence of such ambient interactions. To this end, we partitioned the experimental data in Fig 8 into two sets corresponding to the X and Y tests scenarios (i.e., at t = 45 minutes) and performed the similar correlation and Bland-Altman analyses. Correlation analyses demonstrate strong correlation between the T_es and both T_tm,L (r = 0.994, SSE = 0.033, P < 0.001) and T_tm,R (r = 0.995, SSE = 0.036, P < 0.001) for X, and T_tm,L (r = 0.98, SSE = 0.21, P < 0.001) and T_tm,R (r = 0.998, SSE = 0.078, P < 0.001) for Y. Bland-Altman analyses indicate values of MD and 1.96SD between the T_es and both T_tm,L (MD = −0.978°C, 1.96SD = −0.647°C), and T_tm,R (MD = −0.999°C, 1.96SD = −0.763°C) for X, and T_tm,L (MD = −1.56°C, 1.96SD = −1.141°C), and T_tm,R (MD = −0.95°C, 1.96SD = −0.562°C) for Y. The values of SSE and |MD| prior to the introduction of left face fanning (X test scenario) are relatively small at 0.033 and 0.978°C respectively for left ear, and at 0.036 and 0.999°C respectively for right ear, and both left and right ear have very similar values. With the introduction of fanning (Y test scenario), SSE and |MD| are 0.21 and 1.56°C respectively for left ear, and at 0.078 and 0.95°C respectively for right ear. We compare data for both ears during fanning and observe that SSE and |MD| are relatively higher for the left ear than the right ear. This issue highlights the difficulty of using a soft approach to infer the CBT solely from T_tm when it is affected by other factors such as environmental disturbances in this case. Measurements of these disturbances are necessary to serve as additional inputs to the model. To minimize these effects, a spot on the tympanic membrane which is resilient to these effects would be necessary.

Middle ear cavity as CBT site

Fig 9 reflects temperature measurements from Experiment 3A at the various sites: (1) Rectum, measuring T_rm (reference point), (2) Left middle ear cavity, measuring T_me, and (3) Right tympanic membrane, measuring T_tm,R.

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Fig 9. Temperature measurements results from Experiment 3A.

T_rm, T_me and T_tm,R vs. Time plots that depict behavior of each measurement site in Experiment 3A (No general anesthesia disruption).

https://doi.org/10.1371/journal.pone.0174120.g009

Correlation analyses between T_tm,R and T_rm, and between T_me and T_rm indicate very strong correlations (r → 1) between the T_rm and both the T_tm,R (r = 0.9997, SSE = 0.03, p < 0.001) and the T_me (r = 0.9997, SSE = 0.029, p < 0.001). Bland-Altman analyses show the MD between these temperatures (T_tm,R − T_rm and T_me − T_rm) are −0.968°C and −0.931°C, and the 1.96SD are −0.855°C and −0.835°C, respectively. From the analyses, we observe for all the temperature pairs, their correlation coefficient r → 1, which suggest strong correlation for each temperature pair, indicating T_me and T_tm,R correlate very well with T_rm. The details of the analyses are furnished in Figs B1–B2 in S1 File.

In Experiment 3B, the effects of general anesthesia disruption and the corresponding change in CBT were observed from temperature measurements at various sites. The procedures of Experiment 3A were again carried out and in addition, a probe was inserted into the esophagus to measure T_es. At 2 hours and 51 minutes into the experiment, the administration of general anesthesia was stopped for 15 minutes. Fig 10 shows the measurements at all the selected sites during the time window when general anesthesia was disrupted.

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Fig 10. Temperature measurements results from Experiment 3B.

T_rm, T_me, T_tm,R and T_es vs. Time plots that depict behavior of each measurement site with general anesthesia being disrupted in Experiment 3B.

https://doi.org/10.1371/journal.pone.0174120.g010

It is well-known that the administration of general anesthesia will lower CBT [1, 9]. This was observed at the start of all experiments when general anesthesia was administered. Temperature measurements at all sites in steady state were well below the healthy temperature range. After general anesthesia was halted in Experiment 3B, we observed from Fig 10, a clear and steeper change in the gradient of T_me (marked with a circle in the figure) showing an increase in the rate of change of T_me. This observation is consistent with the presumption that the primate’s body thermoregulation function was gradually being restored with the removal of general anesthesia. This phenomenon is only observed in the T_me measurements and not evident from a visual inspection of other measurements over the same duration.

We split the data in Fig 10 into two measurement groups separated at t = 2.85 hours (2 hours and 51 minutes) boundary: (1) G₁—Primate was administered with general anesthesia in a continuous and controlled manner, and (2) G₂—General anesthesia administration to the primate was halted for 15 minutes.

The data in G₁ group was first analyzed. Correlation analysis demonstrates strong correlation between T_es and T_me (r = 0.995, SSE = 0.009, p < 0.001). Bland-Altman analysis shows the mean difference between temperatures T_es and T_me is +0.812°C, and the 1.96SD is +0.851°C. The data in G₂ group was next analyzed. Correlation analysis still demonstrates a strong correlation between T_es and T_me (r = 0.971, SSE = 0.02, p < 0.001). Bland-Altman analysis shows the mean difference between temperatures T_es and T_me is +0.852°C, and the 1.96SD is +0.915°C.

For comparison purpose, similar analyses were conducted for (T_es, T_tm,R) for both G₁ and G₂ data groups. The details of the conducted analyses are furnished in Figs B3–B6 in S1 File. A summary of the analyses results are shown in Table 1 with consistent p < 0.001 for all. We observe from the table, r ≥ 0.99, and SSE ≈ 0.01 for all the temperature pairs in the G₁ group, showing they are very highly correlated for temperature data in the G₁ group.

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Table 1. Correlations and Bland-Altman analyses results for Experiment 3B.

https://doi.org/10.1371/journal.pone.0174120.t001

We observe a small drop in r, and increase in SSE values when we analyzed the data from G₂ group for the correlation pairs of (T_es, T_me). Other correlation pairs consistently maintain r ≥ 0.99 and SSE ≈ 0.01 in both G₁ and G₂ groups. Thus, there is a deviation in T_me measurements following the general anesthesia change which is not seen in the other temperature measurements.

In addition, from the results of Experiments 3A and 3B, we compare the differences between T_tm,R and T_me over two separate tests. Measurements of T_tm,R were taken at two different points. We observed from Experiment 3A, the T_me and T_tm,R measurements were closely identical and the temperature measurements from Experiment 3B showed a notably greater difference between them with T_me being the higher measurement in both cases (Fig 10). In Experiments 3A and 3B, the temperature measurement points at the right tympanic membrane were selected differently. The primate’s ear canal is very restrictive (much more so than the human) to the insertion of the probe. There is no luxury of space to visually position the probe. In Experiment 3A, the vet could feel the presence of the bone when he engaged the tympanic membrane with the probe, but in Experiment 3B, there was no ‘hard’ contact feel. Thus, two distinctly different points could be invariably used in the experiments. Hence, the same observation as in Experiment 1 can be made that the T_tm measurement is sensitive to the measurement point.

We also observed from Experiment 3B, T_tm,R measurement was higher that T_es, but in Experiment 2, T_tm,R was consistently lower than T_es. It has been reported that the T_tm is higher than T_es [11, 12, 21]. The differentials between T_tm,R and T_rm are rather similar in both experiments, but the differentials between T_es and T_rm are different. It has been noted in previous studies that T_es measurement is sensitive to the placement of the probe and that a lower temperature can be obtained if it is too high up in the esophagus [10, 11].

Other observations

The results from these experiments suggested that T_tm can be used to infer CBT if the probe is directed at a point on the tympanic membrane where stable measurements can be obtained. Otherwise, the effects on the measurements (such as head cooling) should be considered and used to compensate the actual measurements. The latter may not be efficiently viable from a practical perspective.

The measurements from the middle ear cavity were more responsiveness to changes in CBT. Inferencing a faster dynamical variable T_me from a slower T_tm is not easily achieved as it may require the computation of higher derivatives of the slower measurements and this is not practically viable if there exists significant measurement noise. A calibrated relationship between the two variables in the steady state may be more realistic in a soft model. However, this would require a situation wherein temperatures can be allowed to settle down with no additional heat stress or sink occurring in the process, which may or may not be detectable. Dynamically inferencing the CBT from a more sluggish measurement is thus a challenge which has not been truly resolved. Direct measurements of temperature from the middle ear cavity may represent one approach when real-time and dynamic indications of the CBT is needed.

Conclusions

In this paper, we revisited the tympanic membrane vicinity as a potential measurement site for core body temperature (CBT). Two issues were explicitly targeted and experiments were carried out to verify our hypotheses and previous observations. First, the non-uniformity of temperature on the tympanic membrane surface was shown using a probe positioned at a pre-defined proximity to the tympanic membrane at predefined points. A point in front of the malleus bone (A), a point in the lower anterior quadrant (B) and a point at the distal end of the ear canal (C) were selected for evaluation. Point A registered the highest steady state temperature and showed stability in indicating the CBT. Facial cooling has been verified to affect tympanic membrane temperatures, but this sensitivity can be reduced by directing the measurement probe at the desired point. Secondly, the middle ear cavity was explored for the first time as a CBT site. The rapid responsiveness of the middle ear temperature towards a change in the CBT was observed relative to other sites. From the measurements, it is generally difficult to infer this change in middle ear temperature from the temperatures measured at the other sites. The middle ear cavity appears to be a potential CBT site to explore when real-time continuous measurements of CBT are necessary.

Supporting information

S1 File. Correlation and Bland-Altman analyses for Experiments 2 and 3.

The file contains Figs A1–A6 (Appendix A) that represent Analytical results for Experiment 2, and Figs B1–B6 (Appendix B) that represent Analytical results for Experiment 3.

https://doi.org/10.1371/journal.pone.0174120.s001

(PDF)

Acknowledgments

We would like to express our thanks to our colleagues in the Mechatronics & Automation Laboratory, Department of Electrical and Computer Engineering, National University of Singapore for their assistance in ensuring the successful executions of the experiments.

Author Contributions

Conceptualization: WKY JKWL HYL KKT.
Data curation: WKY.
Formal analysis: WKY JKWL HYL KKT.
Investigation: WKY JKWL HYL CWG KKT.
Methodology: WKY JKWL HYL KKT.
Project administration: WKY CWG KKT.
Resources: WKY JKWL HYL CWG KKT.
Software: WKY KKT.
Supervision: KKT JKWL HYL.
Validation: WKY JKWL HYL KKT.
Visualization: WKY CWG WYL.
Writing – original draft: WKY JKWL HYL KKT.
Writing – review & editing: WKY JKWL HYL KKT.

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Figures

Abstract

Introduction

Exploration focal points

Temperature uniformity in the tympanic membrane vicinity and measurement point selection

Middle ear cavity as CBT site

Design of experiments

Equipment

Temperature probes.

Heat chamber and probes setup.

Procedures

Experiment 1.

Experiment 2.

Experiment 3.

Ethics consideration

Results and discussions

Temperature uniformity in the tympanic membrane vicinity and measurement point selection

Middle ear cavity as CBT site

Other observations

Conclusions

Supporting information

S1 File. Correlation and Bland-Altman analyses for Experiments 2 and 3.

Acknowledgments

Author Contributions

References