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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research
(Published
online : 14-07-2013)
19.
A study on bovine brucellosis in an organized dairy farm
- V. Bhanu Rekha, L. Gunaseelan, A. Subramanian and Gowry Yale
Vet World. 2013; 6(9):
681-685
doi:
10.14202/vetworld.2013.681-685
Abstract
Aim:
To assess the status of bovine brucellosis in an organized dairy
with a past history of Brucella abortions and where
Brucella control measures including test and removal,
calf-hood vaccination (already present adult animals were not
vaccinated), use of semen obtained from a screened bull and
general hygienic measures helps in the control of brucellosis in
the farm have been implemented for the past four years.
Materials and Methods: A total of 195 samples including 89
blood samples, 89 serum samples and 17 milk samples were collected
and analysed by isolation and identification, Polymerase chain
Reaction (PCR), Milk Ring Test (MRT), Rose Bengal Plate Test (RBPT),
Standard Tube Agglutination Test (STAT) and Enzyme Linked
Immnuno-Sorbent assay (ELISA).
Results:
On analysis, all the 89 blood and 17 milk samples turned out to be
negative for culture and PCR. MRT and ELISA tests on all the 17
milk samples and STAT on all the 89 serum samples were also
negative. The percent positives for Brucella antibodies in
serum samples were 4.5 and 6.7 by RBPT and ELISA, respectively. Of
the 17 vaccinated animals, 14 were negative by all the Brucella
antigen and antibody diagnostic tests employed. Amongst the
three vaccinated animals, one animal was positive by RBPT and
I-ELISA and, two animals were positive by I-ELISA alone. On the
other hand, of the 72 non-vaccinated animals, 65 were negative by
all the diagnostic tests employed, three animals were positive by
RBPT and 4 animals were positive by I-ELISA.
Conclusion: The results of our study indicated that a
combination of RBPT and I-ELISA can be successfully used for
screening for brucellosis when the prevalence is low.
Implementation of control measures including test and removal of
the affected, calf-hood vaccination, use of semen obtained from a
screened bull and general hygienic measures help in the control of
brucellosis in the farm.
Keywords: bovine brucellosis, control programmes, diagnostic
tests, ELISA, organized dairy farm, RBPT
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