Comparative analyses on expression of galectins1-4, 7-10 and 12 in first trimester placenta, decidua and isolated trophoblast cells in vitro

Laura Unverdorben¹*, Udo Jeschke¹*, Laura Santoso¹, Simone Hofmann¹, Christina Kuhn¹, Petra Arck² and Stefan Hutter<sup>1

1</sup>Department of Gynecology and Obstetrics, Ludwig Maximilians University of Munich, Munich, Germany and ²Department of Gynecology and Obstetrics, University of Hamburg, Hamburg, Germany
*Contributed equally to this work

Offprint requests to: Prof. Dr. Udo Jeschke, Department of Gynecology and Obstetrics, Maistrasse, Ludwig-Maximilians-University, Munich, Germany, Maistrasse 11, 80377 Munich, Germany. e-mail: udo.jeschke@med.uni-muenchen.de

Summary. Introduction: Galectins are members of the mammalian β-galactoside-binding proteins, which recognize Galβ1-4GlcNAc sequences of several cell surface oligosaccharides. Plenty of galectins are already described in human tissue, especially in placenta. Here, gal-1-4, 7-10 and gal-12 were investigated systematically in trophoblast and decidua cells of first trimester placentas. Material and methods: Within this study, 15 first trimester placentas after induced abortion (7th-14th week of gestation) were examined with immunohistology and immunofluorescence based on a scoring system. Moreover, isolated and cultivated trophoblast cells from the first trimester were analyzed and evaluated for expression of gal-1-4, gal-7-10 and gal-12 at mRNA and protein level with real-time RT-Polymerase chain Reaction/PCR (Taq-Man). Double immunofluorescence with trophoblast specific markers identified galectin expressing cells at the feto-maternal interface. Results: We could detect immunohistochemical staining of galectins 1-4, 7-10 and 12 in first trimester placenta: all examined galectins were found in the cytotrophoblast (CTB) and syncytiotrophoblast (SCT). Gal-1, -2, -3, -4, -7, -8, -9, -10 and -12 were identified in extravillous trophoblast cells (EVT) in immunohistology and immunoflourescence. The expression of gal-1, -9, -10, and gal-12 increased after 96h incubation in vitro without stimulation at mRNA level, while gal-2, -3, -4, -7 and -8 were decreased. Discussion and conclusion: This study describes a systematic analysis of the expression of gal-1-4, gal-7-10 and gal-12 in first trimester placentas and isolated trophoblast cells. Expression levels at mRNA level and the change within 96h cultivation in vitro indicate a possible influence on syncytium building of trophoblast cell on expression of galectins. Therefore, an interaction of galectins in vitro in syncytium building is possible. Histol Histopathol 31, 1095-1111 (2016)

Key words: Galectins, Placenta, Tophoblast, Decidua, First trimester

DOI: 10.14670/HH-11-739