Two species of Fragaria, F. nubicola Lindl. ex Lacaita and F. daltoniana J. Gay were cytologically analyzed. Both were diploid (2n=14) and had similar karyotypes consisting of 4 metacentric pairs, 2 submetacentric pairs, and one subtelocentric pair of SAT-chromosomes. The last pair commonly had the shortest length in complements. Furthermore, one plant of F. daltoniana with a small chromosome was observed and details and origin of the karyotype were also described and discussed.