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Data from: Molecular reassessment of diaporthalean fungi associated with strawberry with Paraphomopsis obscurans gen. et comb. nov. (Melanconiellaceae), the cause of leaf blight

dataset
posted on 2023-12-19, 14:15 authored by Dhanushka Udayanga, Shaneya D. Miriyagalla, Dimuthu S. Manamgoda, Kim S. Lewers, Alain Gardiennet, Lisa A. Castlebury

The generic placement of the strawberry leaf blight fungus, formerly known as Phomopsis obscurans has always been subject to uncertainty. These datasets provide the phylogenetic evidence based on four DNA markers (28S rDNA/LSU, ITS, TEF1 and RPB2) that support the establishment of a monotypic new fungal genus Paraphomopsis. Datasets include the single gene sequence alignments for the LSU, ITS, TEF1 and RPB2 markers, and the complete combined phylogenetic dataset and phylogenetic tree files for each single gene and combined analysis. The updated multi-gene datasets and trees for the Diaporthales provide the evidence to distinguish the leaf blight pathogen (Paraphomopsis obscurans) from the taxa associated with leaf blotch (Gnomoniopsis fragariae) and petiole blight and root rot (Paragnomonia fragariae).


Resources in this dataset:

  • Resource Title: Combined LSU_ITS_RPB_TEF1 DNA sequence alignment_Diaporthales.

    File Name: 1_Combined_LSU_ITS_RPB_TEF1_DNA sequence alignment_Diaporthales.txt


  • Resource Title: Combined LSU_ITS_RPB_TEF1 ML tree Diaporthales.

    File Name: 2_Combined tree_LSU_ITS_RPB_TEF1_ML tree_Diaporthales.txt


  • Resource Title: ITS_DNA sequence alignment Diaporthales.

    File Name: 3_ITS_DNA sequence alignment_Diaporthales.txt


  • Resource Title: ITS single gene ML tree Diaporthales.

    File Name: 4_ITS_single gene ML tree_Diaporthales.txt


  • Resource Title: LSU_DNA sequence alignment Diaporthales.

    File Name: 5_LSU_DNA sequence alignment_Diaporthales.txt


  • Resource Title: LSU_single gene ML tree Diaporthales.

    File Name: 6_LSU_single gene ML tree_Diaporthales.txt


  • Resource Title: RPB2_DNA sequence alignment Diaporthales.

    File Name: 7_RPB2_DNA sequence alignment_Diaporthales.txt


  • Resource Title: RPB2_single gene ML tree Diaporthales.

    File Name: 8_RPB2_single gene ML tree_Diaporthales.txt


  • Resource Title: TEF1_DNA sequence alignment Diaporthales.

    File Name: 9_TEF1_DNA sequence alignment_Diaporthales.txt


  • Resource Title: TEF1_single gene ML tree Diaporthales.

    File Name: 10_TEF1_single gene ML tree_Diaporthales.txt

Funding

USDA-ARS: 8042-22000-298-00D

USDA-ARS: 8042-21220-257-00-D

History

Data contact name

Castlebury, Lisa

Data contact email

lisa.castlebury@usda.gov

Publisher

Ag Data Commons

Intended use

These datasets can be used as reference barcodes to identify the strawberry leaf blight fungus.

Temporal Extent Start Date

2015-01-01

Temporal Extent End Date

2018-01-01

Theme

  • Not specified

Geographic Coverage

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Geographic location - description

North America; Europe

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

fungi; strawberries; new combination; leaf blight; Diaporthe; genetic markers; ribosomal DNA; internal transcribed spacers; sequence alignment; genes; trees; pathogens; leaf blotch; petioles; root rot

OMB Bureau Code

  • 005:18 - Agricultural Research Service

OMB Program Code

  • 005:040 - National Research

ARS National Program Number

  • 303

Primary article PubAg Handle

Pending citation

  • No

Public Access Level

  • Public

Preferred dataset citation

Udayanga, Dhanushka; Miriyagalla, Shaneya D.; Manamgoda, Dimuthu S.; Lewers, Kim S.; Gardiennet, Alain; Castlebury, Lisa A. (2020). Data from: Molecular reassessment of diaporthalean fungi associated with strawberry with Paraphomopsis obscurans gen. et comb. nov. (Melanconiellaceae), the cause of leaf blight. Ag Data Commons. https://doi.org/10.15482/USDA.ADC/1518737