[Purpose] The purpose of this study was to determine the relationship between an accelerometer system and a foot pressure sensor system for measuring gait characteristics during walking in healthy adults. [Subjects and Methods] Thirty-five healthy participants with no neurological, musculoskeletal, or cardiopulmonary disorders volunteered for this study. Gait characteristics were measured while participants walked freely along a 10-m walkway using two different measurement systems simultaneously. The first analysis system was based on center of mass using a wireless tri-axial accelerometer and the second system was a foot pressure sensor system. [Results] There was a significant and high correlation between the two systems with respect to gait velocity and cadence. The stride length as a percentage of the stride height measured with the center of mass system was significantly and highly correlated with stride length and stride velocity that was measured with the foot pressure system. Furthermore, stride length from the center of mass system was significantly and highly correlated with stride length and stride velocity from the foot pressure system. [Conclusion] A gait analysis based on a center of mass system is a valid method to assess the effectiveness of therapeutic interventions in a clinical setting.