Recommendations from a Satellite Meeting

Vol. 94, Suppl. I: 429-432, 1999

International Symposium to commemorate the 90th anniversary of the discovery of Chagas disease,

April 11-16 1999, Rio de Janeiro, Brazil

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During this symposium the standardization of the nomenclature of Trypanosoma cruzi strains was discussed, in a parallel session, with a view to facilitating the use and understanding of a common nomenclature that would serve not only taxonomists but the general community of researchers working with T. cruzi.

The diversity in the behavior and morphology of T. cruzi isolates was soon recognized after the discovery of Chagas disease. Since then a variety of biochemical and molecular techniques have revealed the great genetic diversity present in strains of this parasite. Different investigators have described this diversity by using various terms. Correlation between this diversity and the complex epidemiological and clinical manifestations of the disease has however been hindered by the lack of a common nomenclature.

Recent studies have indicated a convergence among investigators regarding the clustering of strains of T. cruzi, into two principal groups. This consensus, together with the report of a meeting on the standardization of methods for T. cruzi classification held in Panama (unpublished document TDR/EPICHA-TCC/85.3 Geneva, WHO, 1985), form the basis of the following recommendations.

1. Isolates and strains of T. cruzi should be named as recommended by the meeting on Chagas disease held in Panama [Rev Soc Bras Med Trop 18 (Supl.): 1985] and the WHO expert committee on the control of Chagas disease (WHO Technical Report Series on Control of Chagas Disease, No. 811, Geneva, 1991) (see Annex A).

2. Isolates and strains of T. cruzi after their characterization by molecular techniques, such as multilocus enzyme electrophoresis (MLEE) and random amplification of polymorphic DNA (RAPD), or genetic loci, such as mini exon genes and 24Sa rDNA, should be classified into the principal groups as follows:

a) strains should be designated as T. cruzi I when they are equivalent to Zymodeme 1 (Miles et al. 1977, 1978, Barrett et al. 1980), Type III (Andrade 1974), Lineage 2 (Souto et al. 1996), Group 1 (Tibayrenc 1995) Ribodeme II/III (Clark & Pung 1994) or similar;

b) strains should be designated as T. cruzi II when they are equivalent to Zymodeme 2 (Miles et al. 1977, 1978, Barrett et al. 1980), Zymodeme A (Romanha et al. 1979), Type II (Andrade 1974), Lineage 1 (Souto et al. 1996), Group 2 (Tibayrenc 1995), Ribodeme I (Clark & Pung 1994) or similar;

c) strains that have not been previously characterized or whose characterization is uncertain can be designated as T. cruzi (without the group designation suffix);

d) the designation of apparent hybrid strains such as those classified as Chilean Zymodeme 2b (Miles et al. 1984), Zymodeme B (Romanha et al. 1979), Type I (Andrade 1974), Group 1/2 typed by 24Sa rDNA (Souto et al. 1996), genotype 39 (Tibayrenc 1995) will be decided later after further studies. A similar situation exists for strains equivalent to Zymodeme 3 (Miles et al. 1978, 1981);

e) T. cruzi I and T. cruzi II will be referred to as groups, while further studies on their evolution and inter- and intra-relationships are carried out and an improved terminology is developed, such as discrete typing unit (DTU) (Tibayrenc 1998);

f) among the institutions offering identification services and listed in Annex 1 of the WHO report mentioned above, the Laboratory of Parasitic Diseases, Department of Tropical Medicine, at the Instituto Oswaldo Cruz, Fiocruz, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brasil, E-mail: octaviof@gene.dbbm.fiocruz.br, tel/fax: +55-21-280.3740, has offered to type uncharacterized strains into the principal groups.

3. The editors of scientific journals should request authors to use the correct nomenclature for strains of T. cruzi (see Annex A) in their manuscripts and where possible designate their strains T. cruzi I, T. cruzi II, or T. cruzi.

4. A further meeting should be held to implement a strain bank(s), a web-site on the internet as well as improve the designation of strain names in this document. In the meantime the web-site of the Memórias do Instituto Oswaldo Cruz where the online version of this document will be held has offered to post a list of strains classified into groups (see Annex B) and to update the list periodically as new results are provided.

Participants: Drs Alejandro Luquetti, Aluízio Prata, Alvaro Moncayo, Alvaro Romanha, Ana Jansen, Bianca Zingales, Carlos Morel, Carlos Ponce, Egler Chiari, Elisa Cupolillo, Elisa Ponce, Felipe Guhl, Hooman Momen, José Rodrigues Coura, Mario Steindel, Michael Miles, Michel Tibayrenc, Octavio Fernandes, Riva Oliveira, Rodrigo Zeledón, Sonia Andrade, Toby Barrett, Vanize Macêdo, Zigman Brener.

Annex A

Designation of isolates and strains

The code for the designation of strains and isolates should consist of four elements, separated by oblique strokes (examples of the four-element code are given in Annex B). The four elements are:

1. the kind of host animal or vector from which the strain was isolated. A four-letter code should be used, the class to which the animal belongs (M for Mammalia) followed by three letters indicating the generic name of the mammalian host or 000 if the host has not yet been identified (in Annex 1 of the WHO report Table A 1.1 gives the code letters to be used for mammalian genera). The WHO report did not provide the code letters for the triatomine vector. Therefore a suggestion for the coding of the vectors is proposed in Annex C;

2. the country in which the isolation was made. The country of isolation is indicated by a two letter code (in Annex 1 of the WHO report Table A 1.2 gives the code letters to be used);

3. the year of isolation. This is indicated by the full four digits. The previous system of using only two digits does not distinguish between the year 2000 and strains whose year of isolation is unknown. These isolates should be referred to as 0000;

4. the laboratory designation (e. g. laboratory code and serial number).

Annex B

Examples of strain designations using the four-element code (followed by the T. cruzi group designation)

MHOM/CO/0000/Colombia (T. cruzi I)

MHOM/BR/1978/Sylvio-X10 (T. cruzi I)

MDID/BR/1982/Dm-28c (T. cruzi I)

TINF/CL/1945/Tulahen (T. cruzi I)

MHOM/BR/1950/Y (T. cruzi II)

MHOM/BR/1962/Berenice (T. cruzi II)

MHOM/BR/1977/Esmeraldo (T. cruzi II)

MHOM/PE/1963/Peru (T. cruzi)

MHOM/BR/1974/12-SF (T. cruzi)

MHOM/BR/1968/Can-III (T. cruzi)

Annex C

Suggested table of species codes for labelling T. cruzi isolates according to the insect host.

Species included

- all New World Triatominae;

Species not included

- insects other than Triatominae;

- Triatominae known only from the Old World (Linshcosteus carnifex, L. chota, L. confumus, L. costalis, L. kali, Triatoma amicitiae, T. bouvieri, T. cavernicola, T. leopoldi, T. migrans, T. pugasi, T. sinica);

Names included

- names recognized as valid by Lent and Wygodzinsky (1979);

- names considered to be junior synonyms by Lent and Wygodzinsky (1979) but which have been in use since 1979;

- names validly published since 1979, whether or not they have subsequently been placed in synonymy;

- for species attributed to more than one genus since 1979, each binomial is listed, but with the same specific code;

- names with incorrect spellings in Lent and Wygodzinsky (1979) have been corrected as follows:

i) Dipetalogaster maximus (Uhler) is listed as Dipetalogaster maxima (Uhler) - mandatory change, article 34 (b), International Code of Zoological Nomenclature, third edition 1985 (-gaster is feminine);

ii) Cavernicola pilosa Barber (incorrect original spelling) is listed as Cavernicola pilosus Barber - justified emendation, Article 32 (d) (- cola is masculine).

Formation of three-letter species codes

The following simple rules were found to be sufficient for assigning a unique code to each of the species listed.

I) The first three letters of the specific name were used (e.g. BAR for Triatoma barberi) unless this would result in duplication.

II) If different names share an initial triplet, a letter unique to the rest of the specific name is chosen as the third letter of the triplet (e.g. COL for Triatoma costalimai and COC for Belminus costaricensis). If this is not possible, or if this would result in a triplet corresponding to the initial letters of another species on the list, one of the species is assigned a code as in I) above. Thus BRK for Triatoma brailovskyi and BRA for Triatoma brasiliensis. BRE is avoided in deference to Rhodnius brethesi and Triatoma breyeri.

III) If different species have the same specific name, the first letter of the genus is used as the third letter of the code (e.g. HEB for Belminus herreri and HEP for Panstrongylus herreri) unless the resulting triplet is preoccupied (thus LEV for Cavernicola lenti, because LEC is the code for Triatoma lecticularia).

Four-letter species codes

It is suggested that the first letter should be T for Triatominae rather than I for Insecta, to avoid duplication with the codes used for Leishmania isolates

Species Genus Sugessted Code arthuri Psammolestes ART arthurneivai Triatoma ARN barberi Triatoma BAR bassolsae Triatoma BAS bolivari Triatoma BOL borbai Microtriatoma BOR brailovskyi Triatoma BRK brasiliensis Triatoma BRA brethesi Rhodnius BRT breyeri Triatoma BRY bruneri Triatoma BRU carcavalloi Triatoma CAL carioca Parabelminus CAC carrioni Triatoma CAN chinai Panstrongylus CHI circummaculata Triatoma CIR coreodes Psammolestes COR costalimai Triatoma COL costaricensis Belminus COC cuspidatus Eratyrus CUS dalessandroi Rhodnius DAL deanei Triatoma DEA delpontei Triatoma DEL diasi Panstrongylus DIA dimidiata Triatoma DIM dispar Triatoma DIS domesticus Rhodnius DOM ecuadorensis Rhodnius ECU eratyrusiformis Triatoma ERA flavida Triatoma FLA funera Triatoma FUN gajardoi Mepraia GAJ galliardi Triatoma GAL garciabesi Triatoma GAR geniculatus Panstrongylus GEN gerstaeckeri Triatoma GER gomeznunezi Triatoma GOM goyovargasi Alberprosenia GOY guasayana Triatoma GUS guazu Triatoma GUZ guentheri Panstrongylus GUE hegneri Triatoma HEG herreri Belminus HEB herreri Panstrongylus HEP hirsuta Paratriatoma HIR howardi Panstrongylus HOW humeralis Panstrongylus HUM incrassata Triatoma INC indictiva Triatoma IND infestans Triatoma INF jurbergi Triatoma JUR laportei Belminus LAP lecticularia Triatoma LEC lenti Cavernicola LEV lenti Panstrongylus LEP lenti Triatoma LET lignarius Panstrongylus LIG limai Triatoma LIM longipennis Triatoma LON lutzi Panstrongylus LUT maculata Triatoma MAC malheiroi Alberprosenia MAL mansosotoi Microtriatoma MAN martinezi Torrealbaia MAR matogrossensis Triatoma MAG matsunoi Hermanlentia MAU matsunoi Triatoma MAU maxima Dipetalogaster MAX mazzottii Triatoma MAZ megistus Panstrongylus MEG melanocephala Triatoma MEC melanosoma Triatoma MES mexicana Triatoma MEX mucronatus Eratyrus MUC nasutus Rhodnius NAS neglectus Rhodnius NEG neivai Rhodnius NEI neotomae Triatoma NEO nigromaculata Triatoma NIG ninioi Triatoma NIN nitida Triatoma NIT obscura Triatoma OBS oliveirai Triatoma OLI pallescens Rhodnius PAC pallidipennis Triatoma PAP paraensis Rhodnius PAR patagonica Triatoma PAT peninsularis Triatoma PEN peruvianus Belminus PER petrochii Triatoma PET phyllosoma Triatoma PHY pictipes Rhodnius PIS picturata Triatoma PIR pilosus Cavernicola PIL pittieri Belminus PIT platensis Triatoma PLA prolixus Rhodnius PRX protracta Triatoma PRT pseudomaculata Triatoma PSE recurva Triatoma REC robustus Rhodnius ROB rubida Triatoma RUD rubrofasciata Triatoma RUS rubrovaria Triatoma RUV rufotuberculatus Panstrongylus RUF rugulosus Belminus RUG ryckmani Triatoma RYC salazari Psammolestes SAL sanguisuga Triatoma SAN scabrosa Bolbodera SCA sinaloensis Triatoma SIN sordida Triatoma SOR spinolai Mepraia SPI spinolai Triatoma SPI stali Rhodnius STA tertius Psammolestes TER tibiamaculata Triatoma TIB trinidadensis Microtriatoma TRI tupynambai Panstrongylus TUP turpiali Panstrongylus TUR venosa Triatoma VEN vitticeps Triatoma VIT williami Triatoma WIL wygodzinskyi Triatoma WYG yurupucu Parabelminus YUR

Notes: bibliographic references for names, descriptions and synonymies can be found in Carcavallo et al. 1998, Jurberg and Galvão 1997, Lent and Wygodzinsky 1979.

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