Cross-Species Utility of Microsatellite Markers in Trichostrongyloid Nematodes

Nicholas D. Temperley; Lucy M. I. Webster; Aileen Adam; Lukas F. Keller; Paul C. D. Johnson

doi:10.1645/GE-1624.1

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Nicholas D. Temperley,^1,2 Lucy M. I. Webster,^2,3 Aileen Adam,² Lukas F. Keller,^2,4 Paul C. D. Johnson^2,5

¹Division of Genetics and Genomics, Roslin Institute (Edinburgh), Roslin, Midlothian EH25 9PS, U.K. nick.temperley@bbsrc.ac.uk
²Division of Environmental and Evolutionary Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, U.K.
³School of Biological Sciences, University of Aberdeen, Aberdeen, AB24 2TZ, U.K.
⁴Zoological Museum, University of Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland
⁵Robertson Centre for Biostatistics, Boyd Orr Building, University of Glasgow, University Avenue, Glasgow G12 8QQ, U.K.

Abstract

The development of microsatellite markers for parasitic nematodes has been hampered by technical difficulties in isolation and PCR amplification. We have investigated the potential for circumventing these problems using microsatellites from 3 trichostrongyloid species on a panel of 7 species. Ten of the 22 PCR primer pairs tested amplified in species other than the target species, usually in closely related species, and 2 new variable loci were discovered in the sheep parasite Trichostrongylus vitrinus. This study provides evidence that cross-species testing of microsatellite primers can be an effective alternative to isolation de novo.

Citation Download Citation

Nicholas D. Temperley, Lucy M. I. Webster, Aileen Adam, Lukas F. Keller, and Paul C. D. Johnson "Cross-Species Utility of Microsatellite Markers in Trichostrongyloid Nematodes," Journal of Parasitology 95(2), 487-489, (1 April 2009). https://doi.org/10.1645/GE-1624.1

Published: 1 April 2009

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