Plant Protect. Sci., 2010, 46(4):145-148 | DOI: 10.17221/10/2010-PPS

Analysis of genetic diversity and phylogeny of partial coat protein domain in Czech and Italian GFLV isolates

Aleš EICHMEIER, Miroslav BARÁNEK, Miroslav PIDRA
Faculty of Horticulture in Lednice, Mendeleum - Institute of Genetics, Mendel University in Brno, Lednice, Czech Republic

The genetic diversity of Grapevine fanleaf virus (GFLV) was evaluated in 4 isolates sampled from naturally infected grapevines from South Moravia (Czech Republic) and 2 Italian isolates from Bari (Italy). Conserved regions within sequences in databases were found and new primers corresponding to these regions were designed and tested for RT-PCR amplification of the CP codifying region. After sequencing of obtained amplicons the similarity of isolates was analysed via alignments of sequences and by means of dendrograms

Keywords: Grapevine fanleaf virus; grapevine; coat protein; sequence

Published: December 31, 2010  Show citation

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EICHMEIER A, BARÁNEK M, PIDRA M. Analysis of genetic diversity and phylogeny of partial coat protein domain in Czech and Italian GFLV isolates. Plant Protect. Sci.. 2010;46(4):145-148. doi: 10.17221/10/2010-PPS.
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