Czech J. Anim. Sci., 2004, 49(3):99-107 | DOI: 10.17221/4286-CJAS

A relationship between the PCR-RFLP polymorphism in porcine MYOG, MYOD1 and MYF5 genes and microstructural characteristics of m. longissimus lumborum in Pietrain × (Polish Large White × Polish Landrace) crosses

D. Kłosowska, J. Kurył, G. Elminowska-Wenda, W. Kapela, K. Walasik, M. Pierzcha, D. Cieślak, J. Bogucka
1 , J. K 2, G. E -W 1, W. K 3, K. W 1, M. P
2 , D. C 2, J. B 1 1Department of Animal Histology, University of Technology and Agriculture, Bydgoszcz, Poland 2Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzębiec, Poland
3 Department of Pig Breeding, University of Technology and Agriculture, Bydgoszcz, Poland

Muscle fibre formation takes place during embryonic development and is regulated by the MyoD gene family, which consists of four genes, MYOD1, myogenin, MYF5 and MRF4. A relationship was studied between MYOD1, myogenin and MYF5 genotypes and microstructural characteristics of the m. longissimus lumborum in pigs - crosses: Pietrain × (Polish Large White × Polish Landrace). The data included 115 unrelated animals slaughtered at about 105 kg live body weight. Within 45 min after exsanguination, samples were taken from the m. longissimus lumborum, frozen in liquid nitrogen and later analysed for the diameter of slow-twitch oxidative, fast-twitch oxidative and fast-twitch low-oxidative fibres, their proportion in a bundle, the proportion of pathological changes and number of fibres per unit area. The RYR1 and MyoD genotypes were determined using the PCR-RFLP technique. A significant or highly significant relation was observed between the diameter of all types of muscle fibres and genotype RYR1 - the highest values were recorded for homozygotes TT (genetically stress-sensitive). A relation between MyoD genotypes and microstructural characteristics of the m. longissimus lumborum was analysed on a group of 93 animals of the genotype CC or CT at locus RYR1. Sex appeared to have no significant effect on the muscle microstructural traits in this group of animals. The content of fast-twitch oxidative fibres (FTO) was significantly related to the MYF5 genotype, whereas that of fast-twitch low-oxidative fibres (FT) was affected by the MYOD1 and MYF5/DdeI genotypes. The proportion of angular fibres in a bundle was related to MYF5/HinfI genotype. The results showed that MyoD genes could be considered as candidate genes for some microstructural characteristics of m. longissimus lumborum in pigs.

Keywords: MyoD genes; muscle fibres; pig; muscle microstructure

Published: March 31, 2004  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Kłosowska D, Kurył J, Elminowska-Wenda G, Kapela W, Walasik K, Pierzcha M, et al.. A relationship between the PCR-RFLP polymorphism in porcine MYOG, MYOD1 and MYF5 genes and microstructural characteristics of m. longissimus lumborum in Pietrain × (Polish Large White × Polish Landrace) crosses. Czech J. Anim. Sci.. 2004;49(3):99-107. doi: 10.17221/4286-CJAS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Ackermann M., Salomon F.V. (1991): Histochemische und morphometrische Befunde am m. longissimus des Schweines bei maligner Hyperthermie. Anat. Histol. Embryol., 20, 280-289.
    2. Anton I., Fésüs L., Zsolnai A. (2002): Simultaneous identification of two MspI polymorphisms of the porcine myogenin gene in Hungarian breeds. J. Anim. Breed. Genet., 119, 280-283. Go to original source...
    3. Bader R. (1987): Histological findings in skeletal muscles of pigs with different stress susceptibility. J. Vet. Med., 34, 452-464. Go to original source... Go to PubMed...
    4. Brocks L., Klont R.E., Buist W., de Greef K., Tieman M., Engel B. (2000): The effects of selection of pigs on growth rate vs leanness on histochemical characteristics of different muscles. J. Anim. Sci., 78, 1247-1254. Go to original source... Go to PubMed...
    5. Brooke M.H., Kaiser K. (1970): Muscle fibre types: How many and what kind? Arch. Neurol., 23, 369-370. Go to original source... Go to PubMed...
    6. Cassens R.G., Cooper C.C., Briskey E.J. (1969): Giant fibre in porcine muscle. Acta Neuropathol., 12, 300-304. Cieślak D., Kapelański W., Blicharski T., Pierzchała M. (2000): Restriction fragment length polymorphisms in myogenin and MYF3 genes and their influence on lean meat content in pigs. J. Anim. Breed. Genet., 117, 43-55. Go to original source...
    7. Cieślak D., Kurył J., Kapelański W., Pierzchała M., Grajewska S., Bocian M. (2002): A relationship between genotypes at MYOG, MYF3 and MYF5 loci and carcass meat and fat deposition traits in pigs. Anim. Sci. Pap. Rep., 20, 77-92.
    8. Comings D.E., MacMurray J.P. (2000): Molecular heterosis: a review. Mol. Genet. Met., 71, 19-31. Go to original source... Go to PubMed...
    9. Coppieters W., Van Zeaveren A., van de Weghe A., Peelman L., Bouquet Y. (1992): Rechtstreekse genotypering von stress (on) gevoelingheid bij verkens met behulp van DNA onderzoek. Vlaams Diergen. Tijds., 61, 68-72.
    10. Dubowitz V., Brooke M.H., Neville H.E. (1973): Muscle Biopsy: A Modern Approach. W.B. Saunders Company Ltd, London, Philadelphia, Toronto.
    11. Dwyer C.M., Fletcher J.M., Stickland N.C. (1993): Muscle cellularity and postnatal growth in the pig. J. Anim. Sci., 71, 3339-3343. Go to original source... Go to PubMed...
    12. Essen-Gustavsson B. (1995): Effects of physical stress on muscle fibre characteristics in pigs - relation to meat quality. In: Proc. 2nd Dummerstorf Muscle Workshop Muscle Growth and Meat Quality, Rostock, Germany, 65-76.
    13. Essen-Gustavsson B., Karlström K., Lundström K. (1992): Muscle fibre characteristics and metabolic response at slaughter in pigs of different halothane genotypes and their relation to meat quality. Meat Sci., 31, 1-11. Go to original source... Go to PubMed...
    14. Fiedler I., Ender K., Wicke M., Maak S., Lengerken G., Meyer W. (1999): Structural and functional characteristics of muscle fibres in pigs with different malignant hyperthermia susceptibility (MHS) and different meat quality. Meat Sci., 53, 9-15. Go to original source... Go to PubMed...
    15. Fujii J., Olsen K., Zorzato F., De Leon S., Khanna V.K., Weiler J.E., O'Brien P.J., Mac Lennan D.H. (1991): Identification of a mutation in porcine ryanodine receptor associated with malignant hyperthermia. Science, 253, 448-451. Go to original source... Go to PubMed...
    16. Gallant E.M. (1980): Histochemical observation on muscle from normal and malignant hyperthermia susceptible swine. Am. J. Vet. Res., 41, 1069-1071.
    17. Greenwood T.A., Kelsoe J.R. (2003): Promoter and intronic variants affect the transcriptional regulation of the human dopamine transporter gene. Genomics, 82, 511-520. Go to original source... Go to PubMed...
    18. Grounds M.D., Garre K.L., Lai M.C., Wright W.E., Bailharz M.W. (1992): Identification of skeletal muscle precursor cells in vivo by use of MyoD1 and myogenin probes. Cell. Tis. Res., 267, 99-104. Go to original source... Go to PubMed...
    19. Handel S.E., Stickland N.S. (1986): Giant muscle fibres in skeletal muscle of normal pigs. J. Comp. Pathol., 96, 447-457. Go to original source... Go to PubMed...
    20. Hasty P., Bradley A., Morris J.H., Edmondson D.G., Venuti J.M., Olson E.N., Klein W.H. (1993): Muscle deficiency and neonatal death in mice with a targeted mutation in the MYOG gene. Nature (Lond.), 364, 501-506. Go to original source... Go to PubMed...
    21. Hausman G., Campion D.R. (1986): Skeletal muscle cellularity and histochemistry in young lean and obese pigs. Growth, 50, 287-295. Go to PubMed...
    22. Henckel P., Oksbjerg N., Erlandsen E., Barton-Gade P., Bejerholm C. (1997): Histo- and biochemical characteristics of the longissimus dorsi muscle in pigs and their relationship to performance and meat quality. Meat Sci., 47, 311-321. Go to original source... Go to PubMed...
    23. Hughes S.M., Taylor J.M., Tapsco S.J., Gurley C.M., Carter W.J., Peterson C.A. (1993): Selective accumulation of MyoD and myogenin mRNAa in fast and slow adult skeletal muscle is controlled by innervation and hormones. Development, 118, 1137-1147. Go to original source... Go to PubMed...
    24. Karlsson A.H., Klont R.E., Fernandez X. (1999): Skeletal muscle fibres as factors for pork quality. Livest. Prod. Sci., 60, 255-269. Go to original source...
    25. Kawasaki E.S. (1990): Sample preparation from blood, cells and others fluids. In: Innis M.A., Gelfand D.H., Sninsky J.J., White T.J. (eds.): PCR Protocols: A Guide to Methods and Applications. Acad. Press, New York. 3-12. Go to original source...
    26. Kłosowska D., Grześkowiak E., Borzuta K., Borys A., Luther R., Elminowska-Wenda G., Hejnowska M. (1998): Influence of pig genotype on histochemical properties of longissimus dorsi muscle and some caracss and meat quality traits. In: Proc. 44th Int. Con. Meat Sci. Technol., Kraków (Poland), 912-913.
    27. Kłosowska D., Kłosowski B., Fiedler I., Wegner J. (1985): Veränderungen in der Fasertypenverteilung und muskelfasergrösse im m. longissimus dorsi der Schweine während des wachstums und Beziehungen zwischen histologischen Merkmalen und Schlachtkörperkriterien. Arch. Tierz., 28, 171-180.
    28. Kłosowska D., Kłosowski B., Kapelański W., Wegner J. (1994): Muscle composition and fibre characteristics in m. longissimus lumborum of the pigs of different breeds. In: Proc. 2nd Int. Conf. The influence of genetic and non-genetic traits on carcass and meat quality, Siedlce (Poland), 218-223.
    29. Kłosowska D., Kłosowski B., Nowak B. (1995): Histopathological changes in longissimus muscle of Pietrain pig and its crosses. In: Proc. 2nd Dummerstorf Muscle - Workshop Muscle Growth and Meat Quality, Rostock, Germany, 84-92.
    30. Knoll A., Nebola M., Dvořak J., Čepica S. (1997): Detection of a DdeI PCR/RFLP within intron 1 od the porcine MYOD1 (MYF3) locus. Anim. Genet., 28, 308-322.
    31. Koishi K., Zhang M., McLennan I.S., Harris A.J. (1995): MyoD protein accumulates in satellite cells and is neurally regulated in regenerating myotubes and skeletal muscle fibers. Dev. Dyn., 202, 244-254. Go to original source... Go to PubMed...
    32. Larzul C., Lefaucheur L., Ecolan P., Gogué J., Talmant A., Sellier P., Le Roy P., Monin G. (1997): Phenotypic and genetic parameters for longissimus muscle fibre characteristics in relation to growth, carcass and meat quality traits in Large White pigs. J. Anim. Sci., 75, 3126-3137. Go to original source... Go to PubMed...
    33. LeHir H., No A., Moore M. (2003): How introns influence and enhance eukaryotic gene expression. Trends Biochem. Sci., 28, 215-220. Go to original source... Go to PubMed...
    34. Maltin C.A., Warkub C.C., Ma hews K.R., Grant C.M., Porter A.D., Delday M.I. (1997): Pig muscle fiber characteristics as a source of variation in eating quality. Meat Sci., 47, 237-248. Go to original source... Go to PubMed...
    35. Miller L.R., Garwood V.A., Judge M.D. (1975): Factors affecting porcine muscle fibre type, diameter and number. J. Anim. Sci., 41, 66-77. Go to original source...
    36. Olson E.N. (1990): MyoD family: A paradigm for development? Gen. Dev., 4, 1454-1461. Go to original source... Go to PubMed...
    37. Rahelic S., Puac S. (1981): Fibre types in longissimus dorsi from wild and highly selected pig breeds. Meat Sci., 5, 451-455. Go to original source... Go to PubMed...
    38. Rehfeldt C., Fiedler I., Dietl G., Ender K. (2000): Myogenesis and postnatal skeletal muscle cell growth as influenced by selection. Livest. Prod. Sci., 66, 177-188. Go to original source...
    39. Rudnicki M.A., Schnegelsberg P.N., Stead R.H., Braun T., Arnold H.H., Jaenisch R. (1993): MyoD or Myf-5 is required for the formation of skeletal muscle. Cell, 75, 1351-1359. Go to original source... Go to PubMed...
    40. Sosnicki A. (1987): Histopathological observations of stress myopathy in m. longissimus in the pig and relationships with meat quality, fa ening and slaughter traits. J. Anim. Sci., 65, 584-596. Go to original source... Go to PubMed...
    41. Soumillion A., Erkens J.H.K., Lenstra J.A., Rettenberger G., Te Pas M.F.W. (1997): Genetic variation in the porcine myogenin gene locus. Mamm. Genome, 8, 564-568. Go to original source... Go to PubMed...
    42. Statistical Analysis Systems Institute (2001): SAS/STAT user's guide, version 8.2. SAS Institute Inc., Cary, NC, USA.
    43. Stratil A., Čepica S. (1999): Three polymorphisms in the porcine myogenic factor 5 (MYF5) gene detected by PCR-RFLP. Anim. Genet. 30, 79-80. Go to original source... Go to PubMed...
    44. Te Pas M.F.W., Visscher A.H. (1994): Genetic regulation of meat production by embryonic muscle formation - a review. J. Anim. Breed. Genet., 111, 404-412. Go to original source... Go to PubMed...
    45. Te Pas M.F.W., Hardens F.L., Soumillion A., Born L., Buist W., Meuwissen T.H.E. (1999a): Genetic variation at the porcine MYF-5 gene locus. Lack of association with meat production traits. Mamm. Genome, 10, 123-127. Go to original source... Go to PubMed...
    46. Te Pas M.F.W., Soumillion A., Hardens F.L., Verburg F.J., van den Bosch T.J., Galesloot P., Meuwissen T.H.E. (1999b): Influences of myogenin genotypes on birth weight, growth rate, carcass weight, backfat thickness and lean weight of pigs. J. Anim. Sci., 77, 2352-2356. Go to original source... Go to PubMed...
    47. Wegner J., Ender K. (1990): Mikrostrukturelle Grundlagen des Wachstums von Muskel und Fe gewebe und die Beziehung zu Fleischansatz und Fleischbeschaffenheit. Fleischwirtscha, 70, 337-340.
    48. Wegner J., Fiedler I., Kłosowska D., Kłosowski B., Ziegan B. (1993): Veranderunden der Muskelfasertypenverteilung in m. longissimus dorsi von Ebern wahrend des Wachstums, dagerstellt mit verschiedinen histochemischen Methoden. Anat. Histol. Embryol., 22, 355-359. Go to original source... Go to PubMed...
    49. Weiler U., Appell H.-J., Kermser M., Hofäcker S., Claus R. (1995): Consequences of selection on muscle composition. A comparative study on gracilis muscle in wild and domestic pigs. Anat. Histol. Embryol., 24, 77-80. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content