Czech J. Genet. Plant Breed., 2011, 47(10):S28-S34 | DOI: 10.17221/3250-CJGPB

New breeding tools impact Canadian commercial farmer fields

R.M. DePAUW1, R.E. KNOX1, D.G. HUMPHREYS2, J.B. THOMAS2, S.L. FOX2, P.D. BROWN2, A.K. SINGH1, C. POZNIAK3, H.S. RANDHAWA4, D.B. FOWLER3, R.J. GRAF4, P. HUCL3
1 Semiarid Prairie Agricultural Research Centre, Agriculture and Agri-Food Canada, Box 1030, Swift Current, S9H 3X2 Saskatchewan, Canada
2 Cereal Research Centre, AAFC, Winnipeg, MB, Canada
3 Crop Development Centre, University of Saskatchewan, Saskatoon, SK, Canada
4 Lethbridge Research Centre, AAFC, Lethbridge, AB, Canada

The high cost of cultivar development encourages efficiencies to reduce time and costs to develop cultivars. Doubled haploid (DH) technology and marker assisted breeding (MAB) are two such tools that improve efficiencies. Since 1997, twenty five wheat cultivars in seven market classes, developed using DH methods, have been registered by the Canadian Food Inspection Agency. These DH cultivars accounted for more than one third of the Canadian wheat acreage in 2009. The DH cultivar Lillian, eligible for grades of Canada Western Red Spring class and currently the most widely grown wheat cultivar in Canada, was developed using MAB to improve grain protein content with the Gpc-B1/Yr36 on chromosome 6BS introgressed from Triticum turgidum L. (Zhuk.) dicoccoides (Körn. Ex Asch. & Graebn). AC Andrew, a Canada Western Soft White spring DH cultivar, was the most widely grown cultivar in its class for the last two years. The new market class, Canada Western Hard White Spring wheat, is based entirely on DH cultivars. Goodeve, one of the first Canada Western Red Spring cultivars released with the gene Sm1 on chromosome 2BS for resistance to the orange wheat blossom midge (Sitodiplosis mosellana (Géhin)) was selected by application of the DNA marker WM1. Glencross, the first cultivar in the Canada Western Extra Strong wheat class with Sm1, was selected using the WM1 marker on haploid plants prior to doubling. Development of the durum wheat cultivars CDC Verona and Brigade involved the use of a marker for Cdu1, a major gene on chromosome 5B that regulates grain cadmium concentration. Marker technology permits a more strategic and integrated approach to breeding by quantifying the introgression of various key genes into advanced breeding material, identifying targeted loci in parents and following up with MAB in the progeny.

Keywords: doubled haploid; field-ready cultivars; marker assisted breeding; wheat

Published: December 31, 2011  Show citation

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DePAUW RM, KNOX RE, HUMPHREYS DG, THOMAS JB, FOX SL, BROWN PD, et al.. New breeding tools impact Canadian commercial farmer fields. Czech J. Genet. Plant Breed.. 2011;47(Special Issue):S28-34. doi: 10.17221/3250-CJGPB.
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References

  1. Aung T., Howes N.K., McKenzie R.I.H., TownleySmith T.F. (1995): Application of the maize pollen method for wheat doubled haploid (DH) generation in western Canadian spring wheat breeding programs. Annual Wheat Newsletter, 41: 70.
  2. Clarke J.M., Leisle D., Kopytko G.L. (1997): Inheritance of cadmium concentration in five durum wheat crosses. Crop Science, 37: 1722-1726. Go to original source...
  3. Clarke J.M., Knox R.E., DePauw R.M., Clarke F.R., McCaig T.N., Fernandez M.R., Singh A.K. (2009): Brigade durum wheat. Canadian Journal of Plant Science, 89: 505-509. Go to original source...
  4. Cuthbert P.A., Somers D.J., Thomas J.B., Cloutier S., Brulé-Babel A. (2006): Fine mapping Fhb1, a major gene controlling Fusarium head blight resistance in bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 112: 1465-1472. Go to original source... Go to PubMed...
  5. DePauw R.M., Townley-Smith T.F., Humphreys G., Knox R.E., Clarke F.R., Clarke J.M. (2005): Lillian hard red spring wheat. Canadian Journal of Plant Science, 85: 397-401. Go to original source...
  6. DePauw R.M., Knox R.E., Clarke F.R., Wang H., Fernandez M.R., Clarke J.M., McCaig T.N. (2007): Shifting undesirable correlations. Euphytica, 157: 409-415. Go to original source...
  7. DePauw R.M., Knox R.E., Thomas J.B., Smith M., Clarke J.M., Clarke F.R., McCaig T.N., Fernandez M.R. (2009): Goodeve hard red spring wheat. Canadian Journal of Plant Science, 89: 937-944. Go to original source...
  8. Fox S.L., Townley-Smith T.F., Humphreys D.G., McCallum B.D., Fetch T.G., Gaudet D.A., Gilbert J.A., Menzies J.G., Noll J.S., Howes N.K. (2006): Somerset hard red spring wheat. Canadian Journal of Plant Science, 86: 163-167. Go to original source...
  9. Gold J., Harder D., Townley-Smith T.F., Aung T., Procunier J. (1999): Development of a molecular marker for rust resistance genes Sr39 and Lr35 in wheat breeding lines. Electronic Journal of Biotechnology, 2: 1-6. Go to original source...
  10. Graf R.J., Hucl P., Orshinsky B.R., Kartha K.K. (2003): McKenzie hard red spring wheat. Canadian Journal of Plant Science, 83: 565-569. Go to original source...
  11. Humphreys D.G., Townley-Smith T.F., Czarnecki E., Lukow O.M., Fofana B., Gilbert J., McCallum B.D., Fetch T., Menzies J. (2007a): Kanata hard red spring wheat. Canadian Journal of Plant Science, 87: 879-882. Go to original source...
  12. Humphreys D.G., Townley-Smith T.F., Czarnecki E., Lukow O.M., Gilbert J., McCallum B.D., Fetch T., Noll J., Brown D. (2007b): Snowbird hard red spring wheat. Canadian Journal of Plant Science, 87: 301-305. Go to original source...
  13. Humphreys D.G., Townley-Smith T.F., Lukow O., McCallum B., Gaudet D., Gilbert J., Fetch T., Menzies J., Brown D., Czarnecki E. (2010): Burnside extra strong hard red spring wheat. Canadian Journal of Plant Science, 90: 79-84. Go to original source...
  14. Jauhar P.P., Xu S.S., Baenziger P.S. (2009): Haploidy in cultivated wheats: induction and utility in basic and applied research. Crop Science, 49: 737-755. Go to original source...
  15. Khan I.A., Procunier J.D., Humphreys D.G., Tranquilli G., Schlatter A.R., Marcucci-Poltri S., Frohberg R., Dubcovsky J. (2000): Development of PCR-based markers for a high grain protein content gene from Triticum turgidum ssp. dicoccoides transferred to bread wheat. Crop Science, 40: 518-524. Go to original source...
  16. Knox R.E., Clarke F.R. (2007): Molecular breeding approaches for enhanced resistance against fungal pathogens. In: Punja Z.K., De Boer S., Sanfacon H. (eds): Biotechnology and Plant Disease Management. CAB Int., Oxfordshire, UK, 321-357. Go to original source...
  17. Knox R.E., Clarke J.M., DePauw R.M. (2000): Dicamba and growth condition effects on doubled haploid production in durum wheat crossed with maize. Plant Breeding, 112: 289-298. Go to original source...
  18. Knox R.E., Pozniak C.J., Clarke F.R., Clarke J.M., Houshmand S., Singh A.K. (2009): Chromosomal location of the cadmium uptake gene (Cdu1) in durum wheat. Genome, 52: 741-747. Go to original source... Go to PubMed...
  19. McCallum B., DePauw R.M. (2008): A review of wheat cultivars grown in the Canadian prairies. Canadian Journal of Plant Science, 88: 649-677. Go to original source...
  20. Penner G.A., Clarke J., Bezte L.J., Leisle D. (1995): Identification of RAPD markers linked to a gene governing cadmium uptake in durum wheat. Genome, 38: 543-547. Go to original source... Go to PubMed...
  21. Pozniak C.J., Fox S.L., Knott D.R. (2009): CDC Verona durum. Canadian Journal of Plant Science, 89: 321-324. Go to original source...
  22. Sadasivaiah R.S., Perkovic S.M., Pearson D.C., Postman B., Beres B.L. (2004): Registration of AC Andrew wheat. Crop Science, 44: 696-697. Go to original source...
  23. Sherman J.D., Lanning S.P., Clark D., Talbert L.E. (2008): Registration of near-isogenic hard- textured wheat lines differing for presence of a high grain protein gene. Journal of Plant, 2: 162-164. Go to original source...
  24. Somers D.J., Humphreys G. (2009): State of QTL detection and marker-assisted selection in wheat improvement. In: Carver B. (ed.): Wheat Science and Trade. Wiley/Blackwell, Ames, 309-326. Go to original source...
  25. Thomas J.B., Fineberg N., Penner G., McCartney C., Aung T., Wise I., McCallum B. (2005): Chromosome location and markers of Sm1: a gene of wheat that conditions antibiotic resistance to orange wheat blossom midge. Molecular Breeding, 15: 183-192. Go to original source...
  26. Townley-Smith T.F., Humphreys D.G., Czarnecki E.M., Lukow O.M., McCallum B.D., Fetch Jr. T.G., Gilbert J., Menzies J.G., Brown P.D. (2010): Superb hard red spring wheat. Canadian Journal of Plant Science, 90: 347-352. Go to original source...
  27. Wiebe K., Pozniak C.J., Harris N., Faris J.D., Clarke J.M., Knox R.E., Taylor G. (2010): Targeted mapping of Cdu1, a major locus regulating grain cadmium concentration in durum wheat (Triticum turgidum L. var. durum). Theoretical and Applied Genetics, 121: 1047-1058. Go to original source... Go to PubMed...
  28. Webliography http://www.inspection.gc.ca/english/plaveg/seesem/seeseme.shtml - The Canadian Food Inspection Agency is responsible for the administration of the Seeds Act and Regulations to help to ensure that seeds sold in, imported into and exported from Canada meet established standards for quality and are labelled so that they are properly represented in the marketplace, and are registered prior to sale in Canada. http://www.pgdc.ca/committees_wrt.html - The Prairie Recommending Committee for Wheat, Rye and Triticale (PRCWRT) evaluates candidate cultivars of wheat, rye and triticale and makes recommendations to the Variety Registration Office, Canadian Food Inspection Agency regarding the suitability of the candidate for registration.

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