Plant Protect. Sci., 2019, 55(3):191-201 | DOI: 10.17221/38/2018-PPS

Characterisation and pathogenicity of Cryphonectria parasitica on sweet chestnut and sessile oak trees in SerbiaOriginal Paper

Dragan Karadžić1, Zlatan Radulović2, Katarzyna Sikora3, Zoran Stanivuković4, Vesna Golubović Ćurguz5, Tomasz Oszako3, Ivan Milenković1,6
1 Department of Forestry, Faculty of Forestry, University of Belgrade, Belgrade, Serbia
2 Institute of Forestry, Belgrade, Serbia
3 Department of Forest Protection, Forest Research Institute - IBL, Sękocin Stary, Poland
4 Department of the Integral Protection of Forest Ecosystems, Faculty of Forestry, University of Banja Luka, Banja Luka, Bosnia and Herzegovina
5 Department of Landscape Architecture and Horticulture, Faculty of Forestry, University of Belgrade, Belgrade, Serbia
6 Phytophthora Research Centre, Mendel University in Brno, Brno, Czech Republic

The presence of Cryphonectria parasitica (Murrill) M.E. Barr was studied in six natural and planted stands of sweet chestnut in Serbia. The fungus was detected on the sweet chestnut in five localities and on the sessile oak in one locality. In total, 77 isolates from the sweet chestnut and five isolates from the sessile oak were obtained. Based on the culture morphology, all the obtained isolates were proven to be free from Cryphonectria (Saccardo) Saccardo & D. Saccardo hypovirus. The isolates of C. parasitica from the sweet chestnut were compatible with three different vegetative compatibility types, EU-12, EU-2, and EU-1, while the isolates from the sessile oak belonged to EU-12. After inoculation in laboratory conditions, the isolate from the sweet chestnut and sessile oak caused the decline in 88 and 76% of the sweet chestnut plants, respectively. In the case of the sessile oak, both isolates caused the decline in 52% of the plants. In field conditions, both isolates were aggressive to sessile oak trees after previous bark wounds and they were statistically significantly different compared to the control trees. The isolate from the sweet chestnut caused significantly larger cankers compared to both the isolate from the sessile oak and the control.

Keywords: chestnut blight fungus; oak decline; sequencing; vegetative compatibility type diversity; aggressiveness

Published: September 30, 2019  Show citation

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Karadžić D, Radulović Z, Sikora K, Stanivuković Z, Golubović Ćurguz V, Oszako T, Milenković I. Characterisation and pathogenicity of Cryphonectria parasitica on sweet chestnut and sessile oak trees in Serbia. Plant Protect. Sci.. 2019;55(3):191-201. doi: 10.17221/38/2018-PPS.
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    References

    1. Adamčíková K., Kobza M., Juhásova G. (2010): Characteristics of the Cryphonectria parasitica isolated from Quercus in Slovakia. Forest Pathology, 40: 443-449. Go to original source...
    2. Adamčíková K., Ondrušková E., Kádasi-Horáková M., Botu M., Kobza M., Achim G. (2015): Distribution and population structure of the chestnut blight fungus in Romania. Plant Protection Science, 51: 141-149. Go to original source...
    3. Bissegger M., Rigling D., Heiniger U. (1997): Population structure and disease development of Cryphonectria parasitica in European chestnut forests in the presence of natural hypo-virulence. Phytopathology, 87: 50-59. Go to original source... Go to PubMed...
    4. Dallavalle E., Zambonelli A. (1999): Epidemiological role of Cryphonectria parasitica isolated from hosts other than chestnut. European Journal of Forest Pathology, 29: 97-102. Go to original source...
    5. EFSA Panel on Plant Health (PLH) (2014): Scientific opinion on the pest categorisation of Cryphonectria parasitica (Murrill) Barr. EFSA Journal 2014, 12: 3859. doi: 10.2903/j.efsa.2014.3859 Go to original source...
    6. Gardes M., Bruns T.D. (1993): ITS primers with enhanced specificity for Basidiomycetes - application to the identification of mycorrhizae and rusts. Molecular Ecology, 2: 113-118. Go to original source... Go to PubMed...
    7. Glišić M.V. (1975): Sweet chestnut (Castanea sativa) in Serbia and its biological and ecological variability. Glasnik Instituta za šumarstvo i drvnu industriju, 36 (Special Issue): 1-211. (in Serbian with French and German summary)
    8. Griffin G.J. (1986): Chestnut blight and its control. Horticultural Reviews, 8: 291-336. Go to original source...
    9. Gryzenhout M., Wingfield B.D., Wingfield M.J. (2009): Taxonomy, Phylogeny, and Ecology of Bark-inhabiting and Tree-pathogenic Fungi in the Cryphonectriaceae. St. Paul, APS Press.
    10. Hall T.A. (1999): BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41: 95-98.
    11. Karadžić D., Milenković I. (2013): Cryphonectria parasitica (Murill.) Barr occurrence of parasitic fungus on sessile oak in Serbia. Šumarstvo, 1-2: 1-8. (in Serbian with English abstract and summary)
    12. Kirk P.M., Cannon P.F., Minter D.W., Stalpers J.A. (2008): Dictionary of the Fungi. 10th Ed. Wallingford, CABI Publishing.
    13. Krstić M. (1950): Endothia parasitica in our country. Zaštita bilja, 2: 113-116. (in Serbian)
    14. Krstin L., Novak-Agbaba S., Rigling D., Ćurković-Perica M. (2011): Diversity of vegetative compatibility types and mating types of Cryphonectria parasitica in Slovenia and occurrence of associated Cryphonectria hypovirus 1. Plant Pathology, 60: 752-761. Go to original source...
    15. Krstin L., Novak-Agbaba S., Rigling D., Krajacic M., Curcovic-Perica M. (2008): Chestnut blight fungus in Croatia: Diversity of vegetative compatibility types, mating types and genetic variability of associated Cryphonectria hypovirus 1. Plant Pathology, 57: 1086-1096. Go to original source...
    16. Marinković P., Karadžić D. (1985): The most important diseases in nurseries, plantations and forests of Kosovo. Šumarstvo, 2-3: 29-38. (in Serbian with English summary)
    17. Mlinarec J., Ježić M., Ćosić J., Ćurković-Perica M. (2018): Multilocus PCR assay reveals high diversity of vegetative compatibility types in populations of Cryphonectria parasitica in Croatia. Plant Pathology, 67: 741-749. Go to original source...
    18. Myteberi I.F., Lushaj A.B., Keča N., Lushaj A.B., Lushaj B.M. (2013): Diversity of Cryphonectria parasitica, hypovirulence, and possibilities for biocontrol of chestnut canker in Albania. International Journal of Microbiology Research and Reviews, 1: 11-21.
    19. Nash B.L., Stambaugh W.J. (1982): Disease incidence, symptomology, and vegetative compatibility type distribution of Endothia parasitica on oak and chestnut hosts in North Carolina. In: Clay Smith H., MacDonald W.L. (eds): Proceedings of the USDA Forest Service American Chestnuts Cooperators Meeting, Jan 5-7, 1982, Morgantown, USA: 74-82.
    20. Perlerou C., Diamandis S. (2006): Identification and geographic distribution of vegetative compatibility types of Cryphonectria parasitica and occurrence of hypovirulence in Greece. Forest Pathology, 36: 413-421. Go to original source...
    21. Radócz L. (2001): Study of subpopulations of the chestnut blight (Cryphonectria parasitica) fungus in the Carpathian basin. Forest Snow and Landscape Research, 76: 368-372.
    22. Radócz L., Tarcali G. (2005): Identification of natural infection of Quercus spp. by chestnut blight fungus (Cryphonectria parasitica). Acta Horticulturae, 693: 617-619. Go to original source...
    23. Radulović Z. (2013): Most often mycosis and pseudomycosis on chestnut, with special review to the species of Cryphonectria parasitica (Murrill) Barr. [Ph.D. Thesis.] Belgrade, University of Belgrade. (in Serbian with English summary)
    24. Risteski M., Milev M., Rigling D., Milgroom M.G., Bryner S.F., Sotirovski K. (2013): Distribution of chestnut blight and diversity of Cryphonectria parasitica in chestnut forests in Bulgaria. Forest Pathology, 43: 437-443. Go to original source...
    25. Robin C., Heiniger U. (2001): Chestnut blight in Europe: Diversity of Cryphonectria parasitica, hypovirulence and biocontrol. Forest, Snow and Landscape Research, 76: 361-367.
    26. Robin C., Anziani C., Cortesi P. (2000): Relationship between biological control, incidence of hypovirulence, and diversity of vegetative compatibility types of Cryphonectria parasitica in France. Phytopathology, 90: 730-737. Go to original source... Go to PubMed...
    27. Shannon C.E., Weaver W. (1949): The Mathematical Theory of Communication. Urbana, University of Illinois Press.
    28. Sotirovski K., Papazova-Anakieva I., Grunwald N., Milgroom M. (2004): Low diversity of vegetative compatibility types and mating type of Cryphonectria parasitica in the southern Balkans. Plant Pathology, 53: 325-333. Go to original source...
    29. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. (2013): MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30: 2725-2729. https://doi.org/10.17221/38/2018-PPS Go to original source... Go to PubMed...
    30. Trestic T., Uscuplic M., Colinas C., Rolland G., Giraud A., Robin C. (2001): Vegetative compatibility type diversity of Cryphonectria parasitica populations in Bosnia-Herzegovina, Spain and France. Forest Snow and Landscape Research, 76: 391-396.
    31. Turchetti T., Maresi G., Santagada A. (1991): Cryphonectria parasitica blight on different hosts in the Cilento region. Monti E Boschi, 42: 54-58. (in Italian)
    32. Tziros G.T., Nakopoulou Z.G., Diamandis S. (2015): Cryphonectria parasitica, the chestnut blight fungus, causes cankers on Quercus frainetto in Greece. Australasian Plant Disease Notes, 10: 19. doi: 10.1007/s13314-015-0171-4 Go to original source...
    33. White T.J., Bruns T., Lee S., Taylor J. (1990): Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis M.A., Gelfand D.H., Sninsky J.J., White T.J. (eds): PCR Protocols: A Guide to Methods and Applications. San Diego, Academic Press: 315-322. Go to original source...

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