Vet Med - Czech, 2006, 51(5):303-310 | DOI: 10.17221/5550-VETMED

Antibiotic resistance of Salmonella spp. isolates from pigs in the Czech Republic

F. Sisak1, H. Havlickova1, H. Hradecka1, I. Rychlik1, I. Kolackova2, R. Karpiskova2
1 Veterinary Research Institute, Brno, Czech Republic
2 National Institute of Public Health, Centre for the Hygiene of Food Chains, Brno, Czech Republic

A total of 126 Salmonella spp. isolates from pigs belonging to 13 serotypes (Typhimurium, Derby, Infantis, Enteritidis, Agona, Kaapstad, London, Montevideo, Bredeney, Give, Oritamerin, Schwarzengrund and Tennessee) were tested for sensitivity to 14 antibiotics. Resistance to 1-8 antibiotics was demonstrated in 64 isolates (59.8%), classified into seven serotypes with the most frequent being Salmonella typhimurium (n = 54). S. typhimurium strains were found to be the most resistant to streptomycin (91.5%), sulphonamides (88.1%), ampicillin (86.4%), tetracycline (84.7%) and chloramphenicol (83.0%), displaying the ACSSuT phenotype. In all strains of this phenotype (n = 27), the gene for integrase (int1) and resistance genes blaPSE-1, floR, aadA2, sul1 and tetG were detected by PCRs. In some of the strains, additional resistance to amoxycillin/clavulanic acid, sulphamethoxazole/trimethoprim, nalidixic acid and enrofloxacin was found.

Keywords: Salmonella serotypes; pig; S. typhimurium; phage type DT104; antibiotic; multiresistance; genes of resistance

Published: May 31, 2006  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Sisak F, Havlickova H, Hradecka H, Rychlik I, Kolackova I, Karpiskova R. Antibiotic resistance of Salmonella spp. isolates from pigs in the Czech Republic. CAAS Agricultural Journals. 2006;51(5):303-310. doi: 10.17221/5550-VETMED.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Akkina J.E., Hogue A.T., Angulo F.J., Johnson R., Petersen K.E., Saini P.K., Fedorka-Cray P.J., Schlosser W.D. (1999): Epidemiologic aspects, control, and importance of multiple-drug resistant Salmonella Typhimurium DT104 in the United States. Journal of the American Veterinary Medical Association, 214, 790-798. Go to PubMed...
    2. Anderson E.S., Ward L.R., Saxe M.J., de Sa J.D. (1977): Bacteriophage-typing designations of Salmonella typhimurium. Journal of Hygiene, 78, 297-300. Go to original source... Go to PubMed...
    3. Anonymous (2003): Directive 2003/99/EC of the European Parliament and of the Council of November 2003 on the monitoring of zoonoses and zoonotic agents, amending Council Decision 90/424/EEC and repealing Council Directive 92/117/EEC. Official Journal of the European Union, L325, 31-40.
    4. Baggesen D.L., Aarestrup F.M. (1998): Characterisation of recently emerged multiple antibiotic-resistant Salmonella enterica serovar Typhimurium DT104 and other multiresistant phage types from Danish pig herds. The Veterinary Record, 143, 95-97. Go to original source... Go to PubMed...
    5. Baggesen D.L., Wegener H.C. (1994): Phage types of Salmonella enterica ssp. enterica serovar Typhimurium isolated from production animals and humans in Denmark. Acta Veterinaria Scandinavica, 35, 349-354. Go to original source... Go to PubMed...
    6. Cloeckaert A., Schwarz S. (2001): Molecular characterization, spread and evolution of multidrug resistance in Salmonella enterica Typhimurium DT104. Veterinary Research, 32, 301-310. Go to original source... Go to PubMed...
    7. Davis M.A., Hancock D.D., Besser T.E. (2002): Multiresistant clones of Salmonella enterica: The importance of dissemination. The Journal of Laboratory and Clinical Medicine, 140, 135-141. Go to original source... Go to PubMed...
    8. Faldynova M., Pravcova M., Sisak F., Havlickova H., Kolackova I., Cizek A., Karpiskova R., Rychlik I. (2003): Evolution of antibiotic resistance in Salmonella enterica serovar Typhimurium strains isolated in the Czech Republic between 1984 and 2002. Antimicrobial Agents and Chemotherapy, 47, 2002-2005. Go to original source... Go to PubMed...
    9. Farrington L.A., Harvey R.B., Buckley S.A., Droleskey R.E., Nisbet D.J., Inskip P.D. (2001): Prevalence of antimicrobial resistance in Salmonellae isolated from market-age swine. Journal of Food Protection, 64, 1496-1502. Go to original source... Go to PubMed...
    10. Gebreyes W.A., Altier C. (2002): Molecular characterization of multidrug-resistant Salmonella enterica subsp. enterica serovar Typhimurium isolates from swine. Journal of Clinical Microbiology, 40, 2813-2822. Go to original source... Go to PubMed...
    11. Gebreyes W.A., Davies P.R., Morrow W.E., Funk J.A., Altier C. (2000): Antimicrobial resistance of Salmonella isolates from swine. Journal of Clinical Microbiology, 38, 4633-4636. Go to original source... Go to PubMed...
    12. Gebreyes W.A., Davies P.R., Turkson P.K., Morrow W.E., Funk J.A., Altier C., Thakur S. (2004): Characterization of antimicrobial-resistant phenotypes and genotypes among Salmonella enterica recovered from pigs on farms, from transport trucks, and from pigs after slaughter. Journal of Food Protection, 67, 698-705. Go to original source... Go to PubMed...
    13. Glynn M.K., Bopp C., Dewitt W., Dabney P., Mokhtar M., Angulo F.J. (1998): Emergence of multidrug-resistant Salmonella enterica serotype Typhimurium DT104 infections in the United States. The New England Journal of Medicine, 338, 1333-1338. Go to original source... Go to PubMed...
    14. Humphrey T. (2001): Salmonella Typhimurium definitive type 104. A multi-resistant Salmonella. International Journal of Food Microbiology, 67, 173-186. Go to original source... Go to PubMed...
    15. Johnson J.M., Rajic A., McMullen L.M. (2005): Antimicrobial resistance of selected Salmonella isolates from food animals and food in Alberta. The Canadian Veterinary Journal, 46, 141-146.
    16. Karpiskova R., Benes D., Dedicova D. (1999): Emergence of multidrug resistant Salmonella thyphimurium DT 104 in the Czech Republic. Eurosurveillance, 4, 56-58. Go to original source... Go to PubMed...
    17. Malorny B., Schroeter A., Helmuth R. (1999): Incidence of quinolone resistance over the period 1986 to 1998 in veterinary Salmonella isolates from Germany. Antimicrobial Agents and Chemotherapy, 43, 2278-2282. Go to original source... Go to PubMed...
    18. Mateu E.M., Martin M., Darwich L., Mejia W., Frias N., Garcia Pena F.J. (2002): Antimicrobial susceptibility of Salmonella strains isolated from swine in Catalonia, Spain. The Veterinary Record, 150, 147-150. Go to original source... Go to PubMed...
    19. Molbak K., Baggesen D.L., Aarestrup F.M., Ebbesen J.M., Engberg J., Frydendahl K., Gerner-Smidt P., Petersen A.M., Wegener H.C. (1999): An outbreak of multidrugresistant, quinolone-resistant Salmonella enterica serotype Typhimurium DT104. The New England Journal of Medicine, 341, 1420-1425. Go to original source... Go to PubMed...
    20. Murphy T.M., McNamara E., Hill M., Rooney N., Barry J., Egan J., O'Connell A., O'Loughlin J., McFaddyen S. (2001): Epidemiological studies of human and animal Salmonella typhimurium DT104 and DT104b isolates in Ireland. Epidemiology and Infection, 126, 3-9. Go to original source... Go to PubMed...
    21. NCCLS (2002): Performance Standards for Antimicrobial Disc and Dilution Susceptibility Tests for Bacteria Isolated from Animals. Approved Standard. 2nd ed. NCCLS document M31-A2. 81 pp.
    22. Nielsen B., Alban L., Stege H., Sorensen L.L., Mogelmose V., Bagger J., Dahl J., Baggesen D.L. (2001): A new Salmonella surveillance and control programme in Danish pig herds and slaughterhouses. Berliner und Munchener Tierarztliche Wochenzeitschrift, 114, 323-326. Go to original source...
    23. Oliveira C.J., Carvalho L.F., Fernandes S.A., Tavechio A.T., Menezes C.C., Domingues F.J., Jr. (2002): Antimicrobial resistance of Salmonella serotypes isolated from slaughter-age pigs and environmental samples. Microbial Drug Resistance, 8, 407-411. Go to original source... Go to PubMed...
    24. Petersen A., Aarestrup F.M., Angulo F.J., Wong S., Stohr K., Wegener H.C. (2002): WHO global salm-surv external quality assurance system (EQAS): an important step toward improving the quality of Salmonella serotyping and antimicrobial susceptibility testing worldwide. Microbial Drug Resistance, 8, 345-353. Go to original source... Go to PubMed...
    25. Poppe C., Smart N., Khakhria R., Johnson W., Spika J., Prescott J. (1998): Salmonella typhimurium DT104: a virulent and drug-resistant pathogen. The Canadian Veterinary Journal, 39, 559-565.
    26. Rajic A., McFall M.E., Deckert A.E., Reid-Smith R., Manninen K., Poppe C., Dewey C.E., McEwen S.A. (2004): Antimicrobial resistance of Salmonella isolated from finishing swine and the environment of 60 Alberta swine farms. Veterinary Microbiology, 104, 189-196. Go to original source... Go to PubMed...
    27. Sisak F., Havlickova H., Karpiskova R., Rychlik I. (2004): Prevalence of Salmonellae and their resistance to antibiotics in slaughtered pigs in the Czech Republic. Czech Journal of Food Science, 22, 230-236. Go to original source...
    28. Sramova H., Karpiskova R., Benes C. (2003): Multiresistant Salmonella enterica subspecies enterica serovar Typhimurium DT104 in the Czech Republic 2000- 2001 (in Czech). Epidemiologie, Mikrobiologie, Imunologie, 52, 66-71.
    29. Swanenburg M., Berends B.R., Urlings H.A., Snijders J.M., Van K.F. (2001): Epidemiological investigations into the sources of Salmonella contamination of pork. Berliner und Munchener Tierarztliche Wochenzeitschrift, 114, 356-359. Go to original source...
    30. Threlfall E.J., Frost J.A., Ward L.R., Rowe B. (1994): Epidemic in cattle and humans of Salmonella typhimurium DT 104 with chromosomally integrated multiple drug resistance. The Veterinary Record, 134, 577. Go to original source... Go to PubMed...
    31. Threlfall E.J., Ward L.R., Rowe B. (1997): Increasing incidence of resistance to trimethoprim and ciprofloxacin in epidemic Salmonella typhimurium DT104 in England and Wales. Eurosurveillance, 2, 81-84. Go to original source... Go to PubMed...
    32. van der Wolf P.J., Bongers J.H., Elbers A.R., Franssen F.M., Hunneman W.A., van Exsel A.C., Tielen M.J. (1999): Salmonella infections in finishing pigs in The Netherlands: bacteriological herd prevalence, serogroup and antibiotic resistance of isolates and risk factors for infection. Veterinary Microbiology, 67, 263-275. Go to original source... Go to PubMed...
    33. Van Winsen R.L., Van Nes A., Keuzenkamp D., Urlings H.A., Lipman L.J., Biesterveld S., Snijders J.M., Verheijden J.H., Van, K.F. (2001): Monitoring of transmission of Salmonella enterica serovars in pigs using bacteriological and serological detection methods. Veterinary Microbiology, 80, 267-274. Go to original source... Go to PubMed...
    34. Yang S.J., Park K.Y., Kim S.H., No K.M., Besser T.E., Yoo H.S., Kim S.H., Lee B.K., Park Y.H. (2002): Antimicrobial resistance in Salmonella enterica serovars Enteritidis and Typhimurium isolated from animals in Korea: comparison of phenotypic and genotypic resistance characterization. Veterinary Microbiology, 86, 295-301. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content