Vet Med - Czech, 2012, 57(4):193-197 | DOI: 10.17221/5916-VETMED

Molecular detection of antimicrobial resistance genes in E. coli isolated from slaughtered commercial chickens in Iran

H. Momtaz1, E. Rahimi1, S. Moshkelani2
1 Faculty of Veterinary Medicine, ShahreKord Branch, Islamic Azad University, ShahreKord, Iran
2 Young Researchers Club, ShahreKord Branch, Islamic Azad University, ShahreKord, Iran

This study was carried out to detect the distribution of antibiotic-resistant genes in Escherichia coli isolates from slaughtered commercial chickens in Iran by PCR. The investigated genes included aadA1, tet(A), tet(B), dfrA1, qnrA, aac(3)-IV, sul1, blaSHV, blaCMY, ere(A), catA1 and cmlA. According to biochemical experiments, 57 isolates from 360 chicken meat samples were recognized as E. coli. The distribution of antibiotic-resistance genes in the E. coli isolates included tet(A) and tet(B) (52.63%), dfrA1, qnrA, catA1 and cmlA (36.84%) and sul1 and ere(A) (47.36%), respectively. Nine strains (15.78%) were resistant to a single antimicrobial agent and 11 strains (19.29%) showed resistance to two antimicrobial agents. Multi-resistance which was defined as resistance to three or more tested agents was found in 64.91% of E. coli strains. The results indicate that all isolates harbour one or more of antibiotic resistance genes and that the PCR technique is a fast, practical and appropriate method for determining the presence of antibiotic-resistance genes.

Keywords: Escherichia coli; chicken meat; antibiotic-resistance genes; PCR; Iran

Published: April 30, 2012  Show citation

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Momtaz H, Rahimi E, Moshkelani S. Molecular detection of antimicrobial resistance genes in E. coli isolated from slaughtered commercial chickens in Iran. Vet Med-Czech. 2012;57(4):193-197. doi: 10.17221/5916-VETMED.
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