Plant Protect. Sci., 2017, 53(1):25-31 | DOI: 10.17221/62/2016-PPS

Genetic population structure of Bemisia tabaci in Spain associated with Tomato leaf curl New Delhi virus - short communicationShort Communication

Dirk JANSSEN*, Almudena SIMON, Oscar CRESPO, Leticia RUIZ
Andalusian Institute for Research and Training in Agriculture, Fisheries, Foods and Organic Production (IFAPA), Centro La Mojonera, La Mojonera, Spain

Tomato leaf curl New Delhi virus (ToLCNDV) originates from Asia where it is persistently transmitted by indigenous cryptic species of the whitefly Bemisia tabaci. The virus has recently invaded Spain, Tunisia, and Italy, and to investigate whether whitefly species new to the Mediterranean are involved, 35 populations were collected during 2015 from different crops in different regions of southern Spain. Comparison of partial mitochondrial cytochrome oxidase I sequences from the collected whiteflies revealed the existence of 7 different haplotypes belonging to the Mediterranean-Q1 cryptic species. ToLCNDV was detected in 15 populations collected from tomato, zucchini, and melon crops and from 5 different localities. The results suggest that MED-Q1 is also responsible for the current spread of ToLCNDV in Spain.

Keywords: whitefly; begomovirus; zucchini squash

Published: March 31, 2017  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
JANSSEN D, SIMON A, CRESPO O, RUIZ L. Genetic population structure of Bemisia tabaci in Spain associated with Tomato leaf curl New Delhi virus - short communication. Plant Protect. Sci.. 2017;53(1):25-31. doi: 10.17221/62/2016-PPS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. CABI (2015): Bemisia tabaci (MEAM1) (silverleaf whitefly). Datasheet. Invasive Species Compendium. Available at www.cabi.org/isc/datasheet/8925
    2. Calvert L.A., Cuervo M., Arroyave J.A., Constantino L.M., Bellotti, A., Frohlich, D. (2001): Morphological and mitochondrial DNA marker analyses of whiteflies (Homoptera: Aleyrodidae) colonizing cassava and beans in Colombia. Annals of the Entomological Society of America, 94: 512-519. Go to original source...
    3. Cenis J.L., Pérez P., Fereres A. (1993): Identification of aphid (Homoptera, Aphididae) species and clones by random amplified polymorphic DNA. Annals of the Entomological Society of America, 86: 545-550. Go to original source...
    4. De Barro P.J., Driver F., Trueman J.W., Curran J. (2000): Phylogenetic relationships of world populations of Bemisia tabaci (Gennadius) using ribosomal ITS1. Molecular Phylogenetics and Evolution, 16: 29-36. Go to original source... Go to PubMed...
    5. De Barro P.J., Ahmed M.Z. (2011): Genetic networking of the Bemisia tabaci cryptic species complex reveals pattern of biological invasions. PLoS ONE, 6 (10), e25579. doi: 10.1371/journal.pone.0025579 Go to original source... Go to PubMed...
    6. De Barro P.J., Liu S.S., Boykin L.M., Dinsdale A.B. (2011): Bemisia tabaci: a statement of species status. Annual Review of Entomology, 56: 1-19. Go to original source... Go to PubMed...
    7. EFSA Panel on Plant Health (2013): Scientific opinion on the risks to plant health posed by Bemisia tabaci species complex and viruses it transmits for the EU territory. EFSA Journal, 11: 3162. Go to original source...
    8. Frohlich D.R., Torres-Jerez I., Bedford L.D., Markham P.G., Brown J.K. (1999): A phylogeographical analysis of the Bemisia tabaci species complex based on mitochondrial DNA markers. Molecular Ecology, 8:1683-1691. Go to original source... Go to PubMed...
    9. Gil-Salas F.M., Morris J., Colyer A., Budge G., Boonham N., Cuadrado I.M., Janssen D. (2007): Development of real-time RT-PCR assays for the detection of Cucumber vein yellowing virus (CVYV) and Cucurbit yellow stunting disorder virus (CYSDV) in the whitefly vector Bemisia tabaci. Journal of Virological Methods, 146: 45-51. Go to original source... Go to PubMed...
    10. Gómez-Menor J. (1943): Contribución al conocimiento de los Aleyródidos de España. (Hem. Homoptera). 1a nota. Eos, 19: 173-209.
    11. Gueguen G., Vavre F., Gnankine O., Peterschmitt M., Charif D., Chiel E., Gottlieb Y., Ghanim M., Zchori-Fein E., Fleury F. (2010): Endosymbiont metacommunities, mtDNA diversity and the evolution of the Bemisia tabaci (Hemiptera: Aleyrodidae) species complex. Molecular Ecology, 19: 4365-4376. Go to original source... Go to PubMed...
    12. Guirao P., Beitia F., Cenis J.L. (1997): Biotype determination of Spanish populations of Bemisia tabaci (Hemiptera: Aleyrodidae). Bulletin of Entomological Research, 87: 587-593. Go to original source...
    13. Jones D.R. (2003): Plant viruses transmitted by whiteflies. European Journal of Plant Pathology, 109: 195-219. Go to original source...
    14. Juárez M., Tovar R., Fiallo-Olivé E., Aranda M.A., Gosálvez B., Castillo P., Moriones E., Navas-Castillo J. (2014): First detection of Tomato leaf curl New Delhi virus infecting zucchini in Spain. Plant Disease, 98: 857. Go to original source... Go to PubMed...
    15. Maruthi, M.N., Rekha A.R., Muniyappa V. (2007): Pumpkin yellow vein mosaic disease is caused by two distinct begomoviruses. Complete viral sequences and comparative transmission by an indigenous Bemisia tabaci and the introduced B-biotype. EPPO Bulletin, 37: 412-419. Go to original source...
    16. Mizutani T., Daryono B.S., Ikegami M., Natsuaki K.T. (2001): First report of Tomato leaf curl New Delhi virus infecting cucumber in Central Java, Indonesia. Plant Disease, 95: 1485. Go to original source... Go to PubMed...
    17. Mnari-Hattab M., Zammouri S., Belkadhi M.S., Bellon Doña D., Ben Nahia E., Hajlaoui M.R. (2015): First report of Tomato leaf curl New Delhi virus infecting cucurbits in Tunisia. New Disease Reports, 31: 21. Go to original source...
    18. Moya A., Guirao P., Cifuentes D., Beitia F., Cenis J.L. (2001): Genetic diversity of Iberian populations of Bemisia tabaci (Hemiptera: Aleyrodidae) based on random amplified polymorphic DNA-polymerase chain reaction. Molecular Ecology, 10: 891-897. doi: 10.17221/62/2016-PPS Go to original source...
    19. Padidam M., Beachy R.N., Fauquet C.M. (1995): Tomato leaf curl geminivirus from India has a bipartite genome and coat protein is not essential for infectivity. Journal of General Virology, 76: 25-35. Go to original source... Go to PubMed...
    20. Panno S., Iacono G., Davino M., Marchione S., Zappardo V., Bella P., Tomassoli L., Accotto G.P., Davino S. (2016): First report of Tomato leaf curl New Delhi virus affecting zucchini squash in an important horticultural area of southern Italy. New Disease Reports, 33: 6. Go to original source...
    21. Ruiz M.L., Simón A., Velasco L., García M.C., Janssen D. (2015): First report of Tomato leaf curl New Delhi virus infecting tomato in Spain. Plant Disease, 99: 894. Go to original source...
    22. Sanchez, J.L., Caoili, B.L. (2015): Identification of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) biotypes using the molecular marker, cytochrome c oxidase I (COI) gene. Philippine Entomologist, 29: 136-156.
    23. Tamura K., Stecher G., Peterson D., Filipski A., Kumar, S. (2013): MEGA6: Molecular Evolutionary Genetics. Analysis Version 6.0. Molecular Biology and Evolution, 30: 2725-2727. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content