Affiliation(s)
ABSTRACT
The Pomegranate (Punica Granatum), which belongs to the Lythraceae family, has been used for
centuries in traditional Greco-Arab and Islamic medicine of its vermifuge
properties and also to treat various diseases. The aim of this research was to investigate
the cytotoxicity and
antioxidant activities of Moroccan Pomegranate (peel, leaves, branches, flowers
and corolla). Further, the biological activities were correlated with
phytochemical contents of the plant extracts.
Methanolic extract from different parts of Punica Granatum was assessed for its
antiproliferative activity in two human cancer (breast and colon) cells lines (MBA-MD 231 and
HT-29), through MTT (3-(4,5-dimethyl-2- thiazolyl)-2,5-diphenyl-2H-tetrazolium
bromide) bioassay using cell viability and cytotoxicity indices. DPPH
(2,2-diphenyl-1-picrylhydrazyl) assay was conducted to screen the antioxidant
property of the extracts together with its phenolic and flavonoids content were
evaluated, as well. The methanolic
extract of Punica Granatum (peel,
leaves, branches, flowers and corolla) showed the highest antiproliferative
activity on MBA-MD 231 (IC50 was 133.53~233.32 μg/mL) and HT-29 (IC50 was 127.58~203.24 μg/mL) cells. Antioxidants contents are distributed as follows: peel
> leaf > flower > corolla > branches. The inhibitory activities required for
decreasing initial DPPH by 50% are 8.27, 9.9, 10.06, 11.67 and 13.28 µg/mL, respectively. These
results are in correlation with polyphenols content from corolla, peel, leaves,
flower and branches are 120.7, 115, 96.65, 90.73 and 64.67 mg GAE/g dw (mg gallic acid
equivalents per g dry weight) and flavonoids are 188.8, 221.7, 180.2, 193.7 and
158.5 mg QE/g dw (mg quercetin equivalents per g dry weight). Our results show that
the peel, flowers, corolla, leaves and branches of Moroccan Pomegranate may
contain a lot of bioactive compounds which are responsible for strong
antioxidant and cytotoxicity activities observed here. Our finding indicates
the possibility of using the extracts of this plant as source of natural antioxidant
and anticancer mainly for its abundant phenolic and flavonoid contents.
KEYWORDS
Punica Granatum,
phytochemical screening, polyphenol, flavonoids, antioxidant activity,
cytotoxic activity.
Cite this paper
Eddebbagh, M., et al. 2016. “Correlation of the Cytotoxic and Antioxidant Activities of Moroccan Pomegranate (Punica Granatum) with Phenolic and Flavonoid Contents.” Journal of Pharmacy and Pharmacology 4 (9): 511-519.
References