ISHS Acta Horticulturae 336: II International Symposium on In Vitro Culture and Horticultural Breeding
CELL SUSPENSION CULTURES IN STRAWBERY: GROWTH CHARACTERIZATION AND VARIABILITY |
| Authors: | F. Blando, A. Niglio, A. Frattarelli, S. Speranza, C. Damiano |
| DOI: | 10.17660/ActaHortic.1993.336.33 |
| Abstract:
An efficient and suitable protocol for cell suspension culture establishment has been defined in the strawberry cultivar Pajaro. Growth and viability characteristics of cells in culture have been analyzed; the sigmoid curve is defined in 20 days of culture, with an initial optimal concentration of 5ml/20 ml (cells/medium). The best medium was composed of B5 Gamborg salts supplemented with 2.3 μM 2,4-dichlorophenoxyacetic acid (2,4-D). Preliminary studies on isoperoxidase activity are also presented; cathodal isoperoxidases did not show any polymorphism related to the growth of cells. | |
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