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Pour, M. S. S., Kasgari, F. H., Farsinejad, A., Fatemi, A., & Khalilabadi, R. M. (2018). Platelet-Derived Microparticles Increase Expression of hTERT in Umbilical Cord Mesenchymal Stem Cells. Research in Molecular Medicine (RMM), 5(4), 31–40. https://doi.org/10.18502/rmm.v5i4.3063

https://doi.org/10.18502/rmm.v5i4.3063

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  • Cited by 3 articles

authors

  • Maryam Samareh Salavati Pour
    No author data available.
  • Fatemeh Hoseinpour Kasgari
    No author data available.
  • Alireza Farsinejad
    No author data available.
  • Ahmad Fatemi
    No author data available.
  • Roohollah Mirzaee Khalilabadi
    No author data available.

Published Date

  • Oct 10, 2018

Platelet-Derived Microparticles Increase Expression of hTERT in Umbilical Cord Mesenchymal Stem Cells

Research in Molecular Medicine (RMM) / Research in Molecular Medicine (RMM): Volume 5, Issue No. 4 / Pages 31–40

Abstract








Introduction: Mesenchymal stem cells (MSCs) are widely studied due to their self- renewal potential and capacity to differentiate into multiple tissues. However, they have a limited life span of several divisions in vitro, which alters various cellular characteristics and reduces their application.


Aim: We evaluated the effect of platelet-derived microparticles on gene expression of hTERT, one of the main factors involved in aging and cell longevity. Materials and methods: Umbilical cord MSCs were used for this study. Cells were characterized by evaluating morphology via inverted microscope and identifying associated surface markers using flow cytometry. Platelet-derived microparticles were prepared by centrifuging platelet bags at varying speeds, and their concen- trations were determined by Bradford assay. At 30% confluency, MSCs were treated with 50 μg/mL of microparticles for five days. Then, RNA was extracted and cDNA was synthesized. Quantitative expression of hTERT was assessed using real-time polymerase chain reaction (PCR).


Results: Fibroblast-like cells were isolated from umbilical cord tissue and MSCs were identified by the presence of mesenchymal surface markers via flow cytometry. Real- time PCR showed that gene expression of hTERT increased by more than three times when treated with platelet-derived microparticles, in comparison to expression of the control group.


Conclusion: We concluded that platelet-derived microparticles may be a potentially safe and effective method to increase hTERT gene expression in MSCs, ultimately prolonging their life span in vitro. 








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