Microarray Technologies for Intracellular Kinome Analysis

Title:Microarray Technologies for Intracellular Kinome Analysis

Volume: 21 Issue: 22

Author(s): T. Yamamoto, T. Mori and Y. Katayama

Affiliation:

Keywords: Antibody, companion diagnosis, diagnosis, drug development, drug screening, intracellular signaling, microarray, molecular targeted drug, order-made medicine, peptide, personalized medicine, prognosis, protein, protein kinase.

Abstract: Microarray-based kinomics, which measure the enzymatic activity or the presence of intracellular protein kinases, are now regarded as alternative tools to conventional mass spectrometry-based kinomics for examining intracellular kinomics. Here, we reviewed the principal advantages, recent progress, and remaining problems of representative microarray- based kinomics, including substrate peptide and protein microarrays, anti-protein kinase antibody microarrays, and reverse protein microarrays. Microarray-based kinomics are not as good at quantitative evaluation of kinomics as the conventional mass spectrometry-based kinomics. However, their simplicity and high throughput make the microarraybased kinomics unique tools, being especially suited for a practical analysis; monitoring drug effects on cellular kinomics as a tool for drug development, and for the diagnosis and prognosis of diseases based on kinomics.

Cite this article as:

Yamamoto T., Mori T. and Katayama Y., Microarray Technologies for Intracellular Kinome Analysis, Current Medicinal Chemistry 2014; 21 (22) . https://dx.doi.org/10.2174/0929867321666131212154153