Abstract
Cancer stem cell-like cells (CSCs) are cancer cells that have the ability of self-renewal and differentiation into multiple malignant cell types (hierarchy). Thus, can cause relapses and metastasis. CSCs’ phenotype is defined by special transcription factors such as Nanog, Oct3/4, Sox2, Nestin, and CD34. The present study aims to determine the change in gene expression of the above markers in correlation with the stage of the disease in breast cancer patients. Initially, whole blood samples from patients with breast cancer were collected, followed by the isolation and culture of circulating tumor cells (CTCs). This was followed by the quantification of CSCs from the above cultures. CSCs’ molecular analysis was performed with qPCR, with the use of gene specific primers. At the same time of the analysis, the clinical assessments of the patients were requested from their physicians. The results indicated a linear relationship between the gene expression of stemness markers and the stage of the disease, as well as specific expression patterns by stage. It seems that these genes have an important role in the progression of the disease, thus they might be target for new treatment approaches.
Keywords: Breast cancer, cancer stem cells, nanog, Sox2, Oct3/4, CD34, nestin, cancer cells, peripheral blood mononuclear cells
Current Stem Cell Research & Therapy
Title:Cancer Stem Cells Stemness Transcription Factors Expression Correlates with Breast Cancer Disease Stage
Volume: 7 Issue: 6
Author(s): Panagiotis Apostolou, Maria Toloudi, Marina Chatziioannou, Eleni Ioannou and Ioannis Papasotiriou
Affiliation:
Keywords: Breast cancer, cancer stem cells, nanog, Sox2, Oct3/4, CD34, nestin, cancer cells, peripheral blood mononuclear cells
Abstract: Cancer stem cell-like cells (CSCs) are cancer cells that have the ability of self-renewal and differentiation into multiple malignant cell types (hierarchy). Thus, can cause relapses and metastasis. CSCs’ phenotype is defined by special transcription factors such as Nanog, Oct3/4, Sox2, Nestin, and CD34. The present study aims to determine the change in gene expression of the above markers in correlation with the stage of the disease in breast cancer patients. Initially, whole blood samples from patients with breast cancer were collected, followed by the isolation and culture of circulating tumor cells (CTCs). This was followed by the quantification of CSCs from the above cultures. CSCs’ molecular analysis was performed with qPCR, with the use of gene specific primers. At the same time of the analysis, the clinical assessments of the patients were requested from their physicians. The results indicated a linear relationship between the gene expression of stemness markers and the stage of the disease, as well as specific expression patterns by stage. It seems that these genes have an important role in the progression of the disease, thus they might be target for new treatment approaches.
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Cite this article as:
Apostolou Panagiotis, Toloudi Maria, Chatziioannou Marina, Ioannou Eleni and Papasotiriou Ioannis, Cancer Stem Cells Stemness Transcription Factors Expression Correlates with Breast Cancer Disease Stage, Current Stem Cell Research & Therapy 2012; 7 (6) . https://dx.doi.org/10.2174/157488812804484639
DOI https://dx.doi.org/10.2174/157488812804484639 |
Print ISSN 1574-888X |
Publisher Name Bentham Science Publisher |
Online ISSN 2212-3946 |
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