Biochemical and histo-morphometrical analyses of bone and cartilage induced by human decalcified dentin matrix and BMP-2

@article{ART001629558},
author={ Masaru Murata and Toshiyuki Akazawa and Junichi Hino and Junichi Tazaki and Katsutoshi Ito and Makoto Arisue },
title={Biochemical and histo-morphometrical analyses of bone and cartilage induced by human decalcified dentin matrix and BMP-2},
journal={Oral Biology Research},
issn={2508-2558},
year={2011},
volume={35},
number={1},
pages={9-14},
doi={10.21851/obr.35.1.201103.9},
url={http://dx.doi.org/10.21851/obr.35.1.201103.9}

TY - JOUR
AU - Masaru Murata
AU - Toshiyuki Akazawa
AU - Junichi Hino
AU - Junichi Tazaki
AU - Katsutoshi Ito
AU - Makoto Arisue
TI - Biochemical and histo-morphometrical analyses of bone and cartilage induced by human decalcified dentin matrix and BMP-2
T2 - Oral Biology Research
PY - 2011
VL - 35
IS - 1
PB - 조선대학교 치의학연구원
SP - 9-14
SN - 2508-2558
AB - The aim of this study was to evaluate the hard tissue-inductive capability by human decalcified dentin matrix (DDM) with or without recombinant human bone morphogenetic protein-2(BMP-2). Human teeth were crushed, completely decalcified and freeze-dried. We named the material DDM. The shape of DDM was a particle type and its size varied from 0.4 to 0.8 mm. The hard tissue induction by 70 mg of DDM was estimated histologically in the nude mice subcutaneous tissue at 4 weeks after implantation. The DDM alone induced bone and cartilage, independently, in the back skin. In addition, the time-course of bone induction by BMP-2 (5.0 μg)/DDM(70 mg) was analyzed in the rat subcutaneous tissues. Histological findings showed that the BMP-2/DDM induced bone and marrow between the DDM particles. Calcium content in the BMP-2/DDMinduced tissue was compatible to the histological findings. The morphometric analysis demonstrated that the BMP-2/DDM showed 66.9%, 79.0% in the volume of bone and marrow, and 32.4%, 21.0% in that of DDM at 8, 32 weeks, respectively. These results indicate that human DDM particles are osteo-chondroinductive and absorbable matrics. Human DDM are effective biomaterials of BMP-2 delivering for bone engineering.
KW - Human, Dentin, Decalcified dentin matrix, BMP-2, Bone induction
DO - 10.21851/obr.35.1.201103.9
UR - http://dx.doi.org/10.21851/obr.35.1.201103.9
ER -

Masaru Murata , Toshiyuki Akazawa , Junichi Hino , Junichi Tazaki , Katsutoshi Ito and Makoto Arisue (2011). Biochemical and histo-morphometrical analyses of bone and cartilage induced by human decalcified dentin matrix and BMP-2. Oral Biology Research, 35( 1), 9- 14.

Masaru Murata , Toshiyuki Akazawa , Junichi Hino , Junichi Tazaki , Katsutoshi Ito and Makoto Arisue . 2011, “Biochemical and histo-morphometrical analyses of bone and cartilage induced by human decalcified dentin matrix and BMP-2”, Oral Biology Research, vol. 35, no. 1, pp. 9-14. Available from: doi:10.21851/obr.35.1.201103.9

Masaru Murata Toshiyuki Akazawa et al. Junichi Hino Junichi Tazaki Katsutoshi Ito Makoto Arisue “Biochemical and histo-morphometrical analyses of bone and cartilage induced by human decalcified dentin matrix and BMP-2” Oral Biology Research 35.1 pp. 9-14 (2011): 9.

Masaru Murata , Toshiyuki Akazawa , Junichi Hino , Junichi Tazaki , Katsutoshi Ito , Makoto Arisue . Biochemical and histo-morphometrical analyses of bone and cartilage induced by human decalcified dentin matrix and BMP-2 Oral Biology Research [Internet]. 2011; 35( 1), : 9-14. Available from: doi:10.21851/obr.35.1.201103.9

Masaru Murata , Toshiyuki Akazawa , Junichi Hino , Junichi Tazaki , Katsutoshi Ito and Makoto Arisue . “Biochemical and histo-morphometrical analyses of bone and cartilage induced by human decalcified dentin matrix and BMP-2” Oral Biology Research 35, no.1 (2011): 9-14. doi: 10.21851/obr.35.1.201103.9

홈 권호 목록 논문 목록 논문 상세

Biochemical and histo-morphometrical analyses of bone and cartilage induced by human decalcified dentin matrix and BMP-2

Oral Biology Research

약어 : Oral Biol Res

2011, vol.35, no.1, pp.9 - 14

DOI : 10.21851/obr.35.1.201103.9

발행기관 : 조선대학교 치의학연구원

연구분야 : 치의학

Masaru Murata¹ , Toshiyuki Akazawa² , Junichi Hino³ , Junichi Tazaki⁴ , Katsutoshi Ito⁵ , Makoto Arisue⁶

¹University of Hokkaido

²University of Hokkaido

³University of Hokkaido

⁴University of Hokkaido

⁵University of Hokkaido

⁶University of Hokkaido

인용한 논문 수 : 4 서지 간략 보기

초록

The aim of this study was to evaluate the hard tissue-inductive capability by human decalcified dentin matrix (DDM) with or without recombinant human bone morphogenetic protein-2(BMP-2). Human teeth were crushed, completely decalcified and freeze-dried. We named the material DDM. The shape of DDM was a particle type and its size varied from 0.4 to 0.8 mm. The hard tissue induction by 70 mg of DDM was estimated histologically in the nude mice subcutaneous tissue at 4 weeks after implantation. The DDM alone induced bone and cartilage, independently, in the back skin. In addition, the time-course of bone induction by BMP-2 (5.0 μg)/DDM(70 mg) was analyzed in the rat subcutaneous tissues. Histological findings showed that the BMP-2/DDM induced bone and marrow between the DDM particles. Calcium content in the BMP-2/DDMinduced tissue was compatible to the histological findings. The morphometric analysis demonstrated that the BMP-2/DDM showed 66.9%, 79.0% in the volume of bone and marrow, and 32.4%, 21.0% in that of DDM at 8, 32 weeks, respectively. These results indicate that human DDM particles are osteo-chondroinductive and absorbable matrics. Human DDM are effective biomaterials of BMP-2 delivering for bone engineering.

키워드

Human, Dentin, Decalcified dentin matrix, BMP-2, Bone induction

참고문헌(15)

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