The Bulletin of Tokyo Dental College
Print ISSN : 0040-8891
Original Article
mRNA Expression and Localization of LPS-induced β-defensin Isoforms in Rat Salivary Glands
Takashi ShinomiyaMitsuru KawaguchiMigiwa OkuboYasuhiro KosugeMasanobu Yoshikawa
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JOURNAL FREE ACCESS

2014 Volume 55 Issue 3 Pages 139-147

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Abstract

β-defensins are small, cationic peptides with broad-spectrum antimicrobial activity that are produced by mucosal epithelia. However, little is known about the expression of β-defensins in the major salivary glands. The purpose of this study was to characterize expression of rat β-defensin-1 (RBD-1) and -2 (RBD-2) mRNA within the major salivary glands together with the effect of injection of intraductal lipopolysaccharide (LPS) on that expression. β-defensin mRNA expression was quantitated by RT-PCR in salivary gland tissues and salivary acinar and striated duct cells collected by laser captured microdissection. RBD-1 and -2 were expressed in the parotid gland, the submandibular gland, and the sublingual gland. β-defensins were expressed in both the acinar and striated duct cells of the major salivary glands. Intraductal injection of LPS increased expression of RBD-1 and -2 mRNA, which peaked at 12 hrs. These results suggest that salivary cells (acinar and striated duct cells) have the potential to produce β-defensins.

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© 2014 by Tokyo Dental College, Japan
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