doiSerbia

Molecular detection of Babesia spp. in ticks in northern Serbia

Mihaljica D. (Laboratory for Medical Entomology, Department for Parasitology, Center of Excellence for Toxoplasmosis and Medical Entomology, Institute for Medical Research, Belgrade)
Radulović Ž. (Laboratory for Medical Entomology, Department for Parasitology, Center of Excellence for Toxoplasmosis and Medical Entomology, Institute for Medical Research, Belgrade)
Tomanović Snežana (Laboratory for Medical Entomology, Department for Parasitology, Center of Excellence for Toxoplasmosis and Medical Entomology, Institute for Medical Research, Belgrade)
Ćakić Sanja (Laboratory for Medical Entomology, Department for Parasitology, Center of Excellence for Toxoplasmosis and Medical Entomology, Institute for Medical Research, Belgrade)
Penezić Aleksandra (Institute of Zoology, Faculty of Biology, Belgrade)
Milutinović Marija (Laboratory for Medical Entomology, Department for Parasitology, Center of Excellence for Toxoplasmosis and Medical Entomology, Institute for Medical Research, Belgrade)

In order to evaluate the prevalence rate of Babesia spp. in ticks collected from vegetation at seven localities in northern Serbia, tick samples were subjected to molecular analysis. A total of 132 unfed adult ticks of five different species (Dermacentor marginatus, Dermacentor reticulatus, Ixodes ricinus, Haemaphysalis concinna and Haemaphysalis punctata), were examined by PCR for the presence of Babesia spp. Out of the analyzed ticks, 10.61% (14/132) were positive for babesial DNA. The presence of babesiae was found at the localities Pančevački Rit, Titov Gaj, Makiš, PKB and Kljajićevo. Prevalence in D. reticulatus ticks was 21.57% (11/51) and in H. concinna ticks, 8.57% (3/35). Sequencing and phylogenetic analysis showed a clustering of the obtained sequences with those of B. canis from the GenBank database. These results add to the knowledge of the distribution of babesial pathogens and their vectors in Serbia.

Keywords: Ticks, PCR, Babesia spp., Serbia

Projekat Ministarstva nauke Republike Srbije, br. ON 173006