doiSerbia

Identification of seed coat phenolic compounds from differently colored pea varieties and characterization of their antioxidant activity

Stanisavljević Nemanja S. (Institute of Molecular Genetics and Genetic Engineering, Belgrade)
Ilić Marija (Institute of Molecular Genetics and Genetic Engineering, Belgrade + Faculty of Chemistry, Belgrade)
Jovanović Živko S. (Institute of Molecular Genetics and Genetic Engineering, Belgrade)
Čupić Tihomir (Agricultural Institute Osijek, Osijek, Croatia)
Dabić Dragana Č. (Faculty of Chemistry Ltd, Innovation Center, Belgrade)
Natić Maja M. (Faculty of Chemistry, Belgrade)
Tešić Živoslav Lj. (Faculty of Chemistry, Belgrade)
Radović Svetlana S. (Faculty of Biology, Belgrade)

The phenolic composition of seed coats in four differently colored pea varieties (Pisum sativum L.) was investigated using UHPLC-LTQ OrbiTrap MS. The obtained findings revealed that the seed coats of the examined pea genotypes possess a unique phenolic composition compared to previously studied European cultivars. In total, 41 phenolic compounds have been identified. The seed coats of the studied cultivars contained certain amounts of rosmarinic acid, rutin, galangin, morin, naringin, hesperetin and pinocembrin as well as ten flavonol glycosides that had not been reported previously. Additionally, the total phenolic content, antioxidant activity and metal chelating capacity of extracts was determined using Folin-Ciocalteu’s method, 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, ferric ion-reducing capacity and ferrous ion-chelating capacity assay, respectively. Dark colored genotypes MBK 168 and MBK 173 possessed the highest total phenolic contents as well the strongest antioxidant activities. On the other hand, bright colored genotypes MBK 88 and MBK 90 exhibited the strongest metal-chelating capacities. The examined pea seed coats may be considered as important potential contributors to human health due to the presence of bioactive phenolic constituents. In addition, our results could be used as a guideline for breeding new pea cultivars with high antioxidant activities applicable in the formulation of functional food products.

Keywords: Pisum sativum L., seed coat, UHPLC-LTQ OrbiTrap MS, phenolic content, antioxidant

Projekat Ministarstva nauke Republike Srbije, br. 173005 i br. 172017