Genetika 2013 Volume 45, Issue 2, Pages: 309-327
https://doi.org/10.2298/GENSR1302309P
Full text ( 443 KB)
Cited by
Genetic differentiation among sexually compatible relatives of Brassica napus L.
Pipan Barbara (Poljoprivredni institut, Odeljenje za oplemenjivanje i semenarstvo, Ljubljana, Slovenija)
Šuštar-Vozlić Jelka (Poljoprivredni institut, Odeljenje za oplemenjivanje i semenarstvo, Ljubljana, Slovenija)
Meglić Vladimir (Poljoprivredni institut, Odeljenje za oplemenjivanje i semenarstvo, Ljubljana, Slovenija)
Analysis of gene flow between Brassica napus L. and its sexually compatible
relatives that could be found in the wild in Slovenia was performed by
microsatellite analysis using fifteen selected primer pairs. Genotypes
included in the study were obtained from the field survey of sexually
compatible relatives of B. napus in natural habitats around Slovenia and from
reference collections. Two different wild species of all the presented
sexually compatible relatives of B. napus were found in Slovenia, B. rapa and
Sinapis arvensis. The reference genotypes included varieties and wild forms
from internal collections as marketable seeds or from gene banks. Reference
genotypes were represented by the following species and subspecies: B. napus
ssp. napobrassica, B. napus ssp. napus, B. nigra, B. oleracea, B. rapa ssp.
oleifera, Diplotaxis muralis; D. tenuifolia, Raphanus raphanistrum, R.
sativus, R. sativus var. oleiformis, Rapistrum rugosum, S. alba and S.
arvensis. Estimation of gene flow described by average number of migrants was
0.72 followed by 0.20 migrants. Due to the observed gene migrations, genetic
drift and selection, Hardy-Weinberg equilibrium was not met. The mean number
of alleles over all loci was 16.9, the average polymorphic information
content was 0.43. We found four highly divergent and polymorphic loci
(Na12-C08, Na10-A08, Ni3-G04b and BRMS-050) at statistically significant
level (p<0.05) of gene flow detected. Over all gene diversity
intra-individual among populations (0.55) was lower than inter-individual
among population (0.77). The results of genetic linkages based standard
genetic distance and unweighted pair group method with arithmetic mean
clustering method, generally divided the genotypes in three divergent groups.
Similar results were obtained by principal coordinate analysis where three
main groups were constructed according to three factors. A real number of
genetic clusters demonstrated a clear separation between populations, where
only one was comprised from genotypes from other origins. The 30.7 %
out-crossing rate of B. rapa and S. arvensis populations from filed survey
represents the potential for spontaneous inter-and intra-specific gene flow
under Slovenian production area.
Keywords: Brassica napus L., sexually compatible relatives, microsatellites, gene flow, genetic diversity, out-crossing rate