Archives of Biological Sciences 2014 Volume 66, Issue 4, Pages: 1333-1338
https://doi.org/10.2298/ABS1404333U
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In vitro propagation and determination of the nutrient content of naturally grown Asparagus tipularis Forssk
Ulukapi Kamile (Akdeniz University, Vocational School of Technical Science, Organic Agriculture Program, Antalya, Turkey)
Nasircilar Ayşe Gül (Akdeniz University, Faculty of Education, Department of Biology Education, Antalya, Turkey)
Onus Ahmet Naci (Akdeniz University, Faculty of Agriculture, Department of Horticulture, Antalya, Turkey)
Baktir İbrahim (Akdeniz University, Faculty of Agriculture, Department of Horticulture, Antalya, Turkey)
Wild Asparagus (Asparagus stipularis Forssk) is a species of the Liliaceae
family. Although its fresh sprouts are collected from natural habitats in
early spring for human diet, it has yet to be cultivated. The present study,
aimed to establish an efficient in vitro propagation system and reveal the
nutritive value of A. stipularis. To our knowledge, there are no reports on
the nutritive value and in vitro propagation of A. stipularis. Bud scale,
spear section and apical bud explants of A. stipularis were cultured on
Murashige and Skoog (MS) medium containing various concentrations of
6-benzylaminopurine (BAP) and α-naphthaleneacetic acid (NAA). The best
results were obtained from apical bud explants on MS medium containing 2.0 mg
l-1 BAP and 0.5 mg l-1 NAA. In terms of nutritive value, it was found that
the nitrogen content of A. stipularis was higher than the other macro
elements. Although Zn- and Fe-containing compounds were higher in A.
stipularis, Ca-containing compounds were lower than in other Asparagus
species. It was determined that the portions of unprocessed A. stipularis had
pH 7.6, 13.3% soluble solid content, 86.7% moisture, 0.09% titratable acidity
and were about 31% ascorbic acid. We conclude that in terms of nutritional
value, A. stipularis can be a good alternative to other Asparagus species for
human nutrition, considering its high N, Fe and vitamin C contents.
Keywords: Asparagus stipularis, in vitro culture, nutrition, nutrition analysis