doiSerbia

Fungal contamination and natural occurrence of ochratoxin A (OTA) in poultry feed

Krnjaja V. (Institute for Animal Husbandry, Belgrade)
Pavlovski Z. (Institute for Animal Husbandry, Belgrade)
Lukić M. (Institute for Animal Husbandry, Belgrade)
Škrbić Z. (Institute for Animal Husbandry, Belgrade)
Stojanović Lj. (Institute for Animal Husbandry, Belgrade)
Bijelić Z. (Institute for Animal Husbandry, Belgrade)
Mandić V. (Institute for Animal Husbandry, Belgrade)

Total fungal count, the presence of potentially toxigenic fungi and natural occurrence of ochratoxin A (OTA) were studied in 30 poultry feed samples (14 samples of feed for chickens and 16 samples of feed for laying hens), which were collected from different farms in Serbia at the beginning of year 2014. The total number of fungi was determined by the method of dilution and OTA was detected using the imunoadsorption enzymatic assay (ELISA). In most of the samples of chickens feed (50%) the total number of fungi was 1 - 3 x 102 CFU g-1, and in feed for laying hens the highest number of samples (37.50%) had the total fungal count from 1.4 to 4.8 x 104 CFU g -1. The species of genera Aspergillus and Penicillium were identified as producers of OTA in 21.43% and 42.86% of chickens feed samples and in 68.75% and 25% of samples of feed for laying hens. The presence of OTA was detected in 100% of samples of feed for chickens and laying hens, with average concentrations of 34.40 μg kg-1 (feed for chickens) and 43.89 μg kg-1 (feed for laying hens). The total fungal count and content of OTA were not above the maximum allowed quantities, even though the presence of Aspergillus and Penicillium species was found in a large number of samples (up to 68.75%). These results indicate that the tested samples of poultry feed were mycologically and mycotoxicologically correct.

Keywords: poultry feed, total fungal count, ochratoxin A (OTA)

Projekat Ministarstva nauke Republike Srbije, br. TR-31023 i br. TR-31033