Genetika 2016 Volume 48, Issue 1, Pages: 285-295
https://doi.org/10.2298/GENSR1601285I
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Characterization of Pseudomonas syringae pathovars from different sweet cherry cultivars by RAPD analysis
Iličić Renata (Faculty of Agriculture, Novi Sad)
Balaž Jelica (Faculty of Agriculture, Novi Sad)
Stojšin Vera (Faculty of Agriculture, Novi Sad)
Jošić Dragana (Institute of Soil Science, Genetic Lab, Belgrade)
Pseudomonas syringae pvs., isolated from sweet cherry grown on different
localities in Serbia, were genetically characterized using RAPD analysis.
Four out of eleven tested primers (SPH1, DJP 17, DJ 15, and DJ 16) were
selected on the basis of the differences between isolates within two
pathovars - syringae and morsprunorum race 1. Cumulative RAPD analysis
indicated heterogeneity within the population of both groups of tested
isolates, revealing four different patterns in each group. RAPD analysis
showed up to 24% differences among pv. syringae isolates, as well as 41% in
comparison with the reference strain KFB0103 (pv. syringae), while
differences of 15% among isolates pv. morsprunorum 1 race and 36% compared to
the reference strain CFBP2119 (pv. morsprunorum 1) were observed. Isolates
from locality Selenca exhibited three different genotypic patterns of pv.
morsprunorum race 1 and one pattern of pv. syringae. Isolates of pv.
morsprunorum collected in the same year from two plant organs (branches and
leaves) of the cv. Vanda yielded two different patterns. The pv. morsprunorum
on cv. Kordia and pv. syringae on cv. Regina were detected at Mikicevo
locality. The same patterns were observed for isolates of pv. syringae from
Kanjiza and Selenca, as well as from Gornji Tavankut in two years of
isolation. Differences were noted between isolates from the same pathovar
originating from Ljutovo and Mikicevo, as well as with respect to all other
isolates of same pathovar.
Keywords: Pseudomonas syringae pv. syringae, P. syringae pv. morsprunorum race 1, sweet cherry, RAPD
Projekat Ministarstva nauke Republike Srbije, br.
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