doiSerbia

Carboxymethyl cellulase production from a Paenibacillus sp.

Mihajlovski Katarina R. (Faculty of Technology and Metallurgy, Department of Biochemical Engineering and Biotechnology, Belgrade)
Davidović Slađana Z. (Faculty of Technology and Metallurgy, Department of Biochemical Engineering and Biotechnology, Belgrade)
Carević Milica B. (Faculty of Technology and Metallurgy, Department of Biochemical Engineering and Biotechnology, Belgrade)
Radovanović Neda R. (Faculty of Technology and Metallurgy, Department of Biochemical Engineering and Biotechnology, Belgrade)
Šiler-Marinković Slavica S. (Faculty of Technology and Metallurgy, Department of Biochemical Engineering and Biotechnology, Belgrade)
Rajilić-Stojanović Mirjana D. (Faculty of Technology and Metallurgy, Department of Biochemical Engineering and Biotechnology, Belgrade)
Dimitrijević-Branković Suzana I. (Faculty of Technology and Metallurgy, Department of Biochemical Engineering and Biotechnology, Belgrade)

Cellulases are industrially important enzymes with a potential to convert cellulose into fermentable sugars. Novel bacterial isolate Paenibacillus sp. CKS1 was tested for cellulase activity and the optimal conditions for carboxymethyl cellulase (CMCase) production were determined. Maximum CMCase activity was obtained in the third passage of the bacterial culture after 3 days of incubation at 30°C. Cellobiose and yeast extract was the optimal source of carbon and nitrogen for induction of CMCase activity. In addition, with initial pH 7 of the medium and 40 ml of working volume in 500 ml culture flasks with shaking at 150 rpm, the maximum CMCase activity in a crude culture supernatant reached value of 0.532±0.006 U/ml. For crude CMCase, optimal temperature was 50°C and optimal pH 4.8, respectively. HPLC analysis confirmed the bacterium is capable to hydrolise CMC to glucose and other soluble sugars.

Keywords: Paenibacillus sp., cellulose, CMCase production, optimal conditions

Projekat Ministarstva nauke Republike Srbije, br. TR 31035