Vojnosanitetski pregled 2016 Volume 73, Issue 12, Pages: 1132-1138
https://doi.org/10.2298/VSP151125013Z
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Histomorphometric evaluation of bone regeneration using autogenous bone and beta-tricalcium phosphate in diabetic rabbits
Živadinović Milka (Faculty of Dental Medicine, Clinic of Oral Surgery, Belgrade)
Andrić Miroslav (Faculty of Dental Medicine, Clinic of Oral Surgery, Belgrade)
Milošević Verica (Institute for Biological Research “Siniša Stanković”, Department of Cytology, Belgrade)
Manojlović-Stojanoski Milica (Institute for Biological Research “Siniša Stanković”, Department of Cytology, Belgrade)
Prokić Branislav (Faculty of Veterinary Medicine, Department for Surgery, Orthopedic and Ophthalmology, Belgrade)
Prokić Bogomir (Faculty of Veterinary Medicine, Department for Surgery, Orthopedic and Ophthalmology, Belgrade)
Dimić Aleksandar (Military Medical Academy, Department of Otorhinolaryngology, Belgrade)
Ćalasan Dejan (Faculty of Dental Medicine, Clinic of Oral Surgery, Belgrade)
Brković Božidar (Faculty of Dental Medicine, Clinic of Oral Surgery, Belgrade)
Background/Aim. The mechanism of impaired bone healing in diabetes mellitus
includes different tissue and cellular level activities due to micro- and
macrovascular changes. As a chronic metabolic disease with vascular
complications, diabetes affects a process of bone regeneration as well. The
therapeutic approach in bone regeneration is based on the use of
osteoinductive autogenous grafts as well as osteoconductive synthetic
material, like a β-tricalcium phosphate. The aim of the study was to
determine the quality and quantity of new bone formation after the use of
autogenous bone and β-tricalcium phosphate in the model of calvarial
critical-sized defect in rabbits with induced diabetes mellitus type I.
Methods. The study included eight 4-month-old Chincilla rabbits with
alloxan-induced diabetes mellitus type I. In all animals, there were
surgically created two calvarial bilateral defects (diameter 12 mm), which
were grafted with autogenous bone and β-tricalcium phosphate (n = 4) or
served as unfilled controls (n = 4). After 4 weeks of healing, animals were
sacrificed and calvarial bone blocks were taken for histologic and
histomorphometric analysis. Beside descriptive histologic evaluation, the
percentage of new bone formation, connective tissue and residual graft were
calculated. All parameters were statistically evaluated by Friedman Test and
post hock Wilcoxon Singed Ranks Test with a significance of p < 0.05.
Results. Histology revealed active new bone formation peripherally with
centrally located connective tissue, newly formed woven bone and well
incorporated residual grafts in all treated defects. Control samples showed
no bone bridging of defects. There was a significantly more new bone in
autogeonous graft (53%) compared with β-tricalcium phosphate (30%), (p <
0.030) and control (7%), (p < 0.000) groups. A significant difference was
also recorded between β-tricalcium phosphate and control groups (p < 0.008).
Conclusion. In the present study on the rabbit grafting model with induced
diabetes mellitus type I, the effective bone regeneration of critical bone
defects was obtained using autogenous bone graft.
Keywords: rabbitis, diabetes mellitus, bone regeneration, transplantation, autologous, beta-tricalcium phosphate
Projekat Ministarstva
nauke Republike Srbije, br. 175021