Aspergillus MO-10, isolated from the bottom mud of Seto Inland Sea grew well in an ordinary sea water medium and produced an antibiotic substance in the culture medium. This substance was adsorbed on to charcoal from the culture filtrate and eluted with chloroform. The substance was crystallized from the ethanol solution after the evaporation of chloroform.
The elemental analysis of the substance gave a calculated value equal to that of gliotoxin(C₁₈H₁₄N₂O₄S₂) and the UV, IR, NMR and MS spectra showed good agreement with those of gliotoxin.
Although the present sample has not been compared with a synthetic specimen of gliotoxin, the analytical and spectroscopic evidence mentioned above indicate that the isolated substance from Aspergillus MO-10 is probably gliotoxin.